Bismuth oxide aqueous colloidal nanoparticles inhibit Candida albicans growth and biofilm formation

Hernandez-Delgadillo, Rene; Velasco‐Arias, Donaji; Martinez-Sanmiguel, Juan Jose; Díaz, David Díaz; Zumeta-Dubé, Inti; Árevalo-Niño, Katiushka; Cabral-Romero, Claudio

doi:10.2147/ijn.s38708

Cited by 41 publications

(21 citation statements)

References 47 publications

(40 reference statements)

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“…Regarding C. albicans , the calculated IC 50 value for BiNPs against biofilms formed by this fungus was 7.9 µg mL -1 , which interestingly is very close to its value under planktonic conditions As far as the authors know, the antibiofilm activity of BiNPs only has been addressed in one other study, where a concentration of 418 µg mL -1 was required to inhibit the C. albicans biofilm. [55]. When compared to antifungal agents.…”

Section: Resultsmentioning

confidence: 99%

Bismuth nanoparticles obtained by a facile synthesis method exhibit antimicrobial activity againstStaphylococcus aureusandCandida albicans

Vázquez-Muñoz

Arellano-Jimenez

Lopez-Ribot

2020

Preprint

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19Bismuth compounds are known for their activity against multiple microorganisms; yet, the antibiotic properties of 20 bismuth nanoparticles (BiNPs) remain poorly explored. The objective of this work is to further the research of 21BiNPs for nanomedicine, particularly as a disinfectant and for future treatments. Stable PVP-coated BiNPs were 22 produced by a chemical reduction process, in less than 30 minutes, in a heated alkaline glycine solution, by the 23 chelation and reduction of the bismuth (III) ion; resulting in the generation of small, spheroid particles with a 24 crystalline organization. We assessed the antibacterial and antifungal activity of bismuth nanoparticles. PVP-25BiNPs showed potent antibacterial activity against the pathogenic bacterium Staphylococcus aureus and 26 antifungal activity against the opportunistic pathogenic yeast Candida albicans, both under planktonic and biofilm 27 growing conditions. Our results indicate that BiNPs represent promising antimicrobial nanomaterials, and this 28 facile synthetic method may allow for further investigation of their activity against a variety of pathogenic 29 microorganisms. 30 31 for years) [1,2]. Bismuth is insoluble in water but soluble in some organic solutions. The water-solubility and 36 lipophilicity of bismuth can be enhanced when it is complexed with lipophilic molecules [3] and its biocompatibility 37 is increased when it is chelated with hydroxyl or sulfhydryl groups. Bismuth compounds have been used in 38 medicine for more than two centuries [4]. Bismuth subsalicylate is utilized to treat diarrhea-related ailments since 39 the 1900s [5], and most recently, bismuth compounds have been used in computed tomography imaging and 40anti-cancer therapy [6,7]. In the U.S., around 30% of the bismuth compounds are used for cosmetic and 41 pharmaceutical applications [4]. 42Several bismuth compounds display antibacterial and antifungal activity [8,9], and can outperform the inhibitory 43 activity of conventional antibiotics [10]. When bismuth is thiolated, such as in the case of bismuth-thiols (BTs) 44 complexes, its antimicrobial activity is further enhanced [11]. Bismuth-dimercaptopropanol (bismuth-BAL) and 45other BTs display anti-biofilm activity [12]. Additionally, the thiolation of bismuth complexes increases their 46 stability [13]; however, BTs stability is still relatively low when compared to other antimicrobial agents [14]. 47 of synthesis, as follows: High-Resolution Transmission Electron Microscopy (HR-TEM) for determining the 96 single-particle structural lattice, using Selected-Area Electron Diffraction (SAED) analysis. The PVP-BiNPs, 97 deposited on Type-B carbon-coated copper grids (Ted Pella Inc.), were examined in a JEOL 2010-F HR-TEM 98 (Jeol Ltd.), with 200 kV accelerating voltage. Dynamic Light Scattering analysis to determine the hydrodynamic 99 size, using a Zetasizer Nano ZS (Malvern Panalytical). PVP-BiNPs were diluted in Milli Q water and then 100 transferred to a DTS1070 cell for the analysis. UV-Vis spectroscopy to follow...

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Section: Resultsmentioning

confidence: 99%

Bismuth nanoparticles obtained by a facile synthesis method exhibit antimicrobial activity againstStaphylococcus aureusandCandida albicans

Vázquez-Muñoz

Arellano-Jimenez

Lopez-Ribot

2020

Preprint

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“…Furthermore, doses of 100 mg mL −1 and 0.932 mg•mL −1 of Bi 2 O 3 NPs induced no cytotoxicity in murine periodontal ligament and rat osteosarcoma cells and monkey kidney cells, respectively. 42 , 43 …”

Section: Toxicitymentioning

confidence: 99%

<p>A Review on the Biodistribution, Pharmacokinetics and Toxicity of Bismuth-Based Nanomaterials</p>

Badrigilan¹,

Heydarpanahi²,

Choupani³

et al. 2020

IJN

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Here, bismuth-based nanomaterials (Bi-based NMs) are introduced as promising theranostic agents to enhance image contrast as well as for the therapeutic gain for numerous diseases. However, understanding the interaction of such novel developed nanoparticles (NPs) within a biological environment is a requisite for the translation of any promising agent from the lab bench to the clinic. This interaction delineates the fate of NPs after circulation in the body. In an ideal setting, a nano-based therapeutic agent should be eliminated via the renal clearance pathway, meanwhile it should have specific targeting to a diseased organ to reach an effective dose and also to overcome off-targeting. Due to their clearance pathway, biodistribution patterns and pharmacokinetics (PK), Bi-based NMs have been found to play a determinative role to pass clinical approval and they have been investigated extensively in vivo to date. In this review, we expansively discuss the possible toxicity induced by Bi-based NMs on cells or organs, as well as biodistribution profiles, PK and the clearance pathways in animal models. A low cytotoxicity of Bi-based NMs has been found in vitro and in vivo, and along with their long-term biodistribution and proper renal clearance in animal models, the translation of Bi-based NMs to the clinic as a useful novel theranostic agent is promising to improve numerous medical applications.

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“…Studies have shown that bismuth derivatives and its nanoparticulate forms inhibit Helicobacter pylori growth by altering their Krebs cycling, amino acid, and nucleotide metabolisms, and they can be used as an antidiarrheal agent to treat nausea, vomiting, and stomach pain [ 22 ]. Additionally, it was reported that BiNPs exhibited antibacterial and antifungal activities at concentrations lower than 1 mM and 2 mM, respectively [ 23 , 24 ]. Also, they can interfere with the biofilm formation of S. mutans , the main etiological agent of dental decays [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Antibacterial Effects of Bismuth Nanoparticles against Enterococcus faecalis

Azad

Rostamifar

Modaresi

et al. 2020

BioMed Research International

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Introduction. Enterococcus faecalis (E. faecalis) is the most important species in dentistry and plays a significant role in the etiology of persistent apical lesions after root canal treatment. Up to date, the intracanal application of 2% chlorhexidine for 7 days is the best way to eliminate E. faecalis. However, due to the ability of this bacterium to persist and survive in harsh environments, many studies have been directed towards finding an alternative strategy for prevention or eradication of it. This study was conducted to investigate the effect of bismuth nanoparticles on E. faecalis, as an etiologic factor in recurrent root canal infections. Methods. Forty patients, referred to Endodontic Ward of Shiraz University of Medical Science for endodontic pretreatment, provided root canal samples. First, all samples were transferred in Enterococcosel broth and incubated. Then, samples which showed growth were plated on blood agar plates and incubated for further PCR procedure. Nanoparticle powder was dissolved in high-purity water, and the final concentration of bismuth nanoparticles (BiNPs) was measured by the spectrophotometer. Minimum inhibitory concentration (MIC) of BiNPs against E. faecalis was determined by microbroth dilution method according to methods for antimicrobial susceptibility tests. Also, bactericidal assays were conducted in Mueller-Hinton broth medium and reported as the concentration of BiNPs that reduced the viable bacterial count by 99.9%. Results. Of all samples, 77.5% revealed the presence of E. faecalis by PCR. Also, E. faecalis growth inhibition was observed at concentrations ranging from 0.625 μg/ml to 20 μg/ml (geometric mean: 2.337 μg/ml), and the MBC values were between 1.25 μg/ml and 40 μg/ml (geometric mean: 4.781 μg/ml), which in comparison with chlorhexidine, these values were about one-eighth of chlorhexidine. Conclusion. The experimental data suggest that bismuth nanoparticles could be an interesting alternative to combat E. faecalis, which, in view of the advantages mentioned for bismuth nanoparticle like inhibiting Streptococcus mutans biofilm formation and higher antibacterial activity compared to chlorhexidine, can be suggested to be used in different fields of dentistry.

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Bismuth oxide aqueous colloidal nanoparticles inhibit Candida albicans growth and biofilm formation

Cited by 41 publications

References 47 publications

Bismuth nanoparticles obtained by a facile synthesis method exhibit antimicrobial activity againstStaphylococcus aureusandCandida albicans

Bismuth nanoparticles obtained by a facile synthesis method exhibit antimicrobial activity againstStaphylococcus aureusandCandida albicans

<p>A Review on the Biodistribution, Pharmacokinetics and Toxicity of Bismuth-Based Nanomaterials</p>

Assessment of the Antibacterial Effects of Bismuth Nanoparticles against Enterococcus faecalis

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