Bis-quaternary ammonium gemini surfactants for gene therapy: Effects of the spacer hydrophobicity on the DNA complexation and biological activity

Acosta-Martínez, Delvis R.; Rodríguez-Velázquez, Eustolia; Araiza-Verduzco, Fernanda; Taboada, Pablo; Prieto, Gerardo; Rivero, Ignacio A.; Pina‐Luis, Georgina; Alatorre-Meda, Manuel

doi:10.1016/j.colsurfb.2020.110817

Cited by 14 publications

(11 citation statements)

References 42 publications

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“…Since the phase separation occurs only after titration, it does not interfere in the respective characteristic absorbances of Au and Ag NPs. Similar titrations with 16-3-16-, 16-4-16-, 16-5-16-, and 16-6-16-stabilized Ag NPs also provided identical results (Figure S2), where the complexation concentration of Ag NPs increases with spacer length (Figure S3), thus indicating a delay in the complexation between Au and Ag NPs as the length of the spacer increases. , …”

Section: Resultssupporting

confidence: 53%

“…Figure 1a shows that SDLC-stabilized Au NPs provide a broad absorbance at 520 S3), thus indicating a delay in the complexation between Au and Ag NPs as the length of the spacer increases. 16,17 Simultaneously, DLS size measurements explain how the spacer effect influences the Au−Ag NP interactions. The colloidal suspension of SDLC-stabilized Au NPs provides a large average size of 485 ± 81 nm (Figure 2a, inset), though a small size close to 50 nm is also observed for a little amount of NPs.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Colloidal Stabilization of Sodium Dilauraminocystine for Selective Nanoparticle–Nanoparticle Interactions: Their Screening and Extraction by Iron Oxide Magnetic Nanoparticles

Kaur¹,

Sandhu²,

Khullar³

et al. 2021

Langmuir

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Nanoparticle−nanoparticle (NP−NP) interactions between Au and Ag NPs were studied by using sodium dilauraminocystine (SDLC)-and Gemini surfactant-stabilized NPs to demonstrate the unique NP surface adsorption behavior of SDLC in controlling and mimicking such interactions in complex mixtures. They were significantly affected by the spacer as well as the polymeric nature of the head group of Gemini surfactants. A longer spacer impeded while a polymeric head group facilitated the interactions. The Au−Ag NPs interactions in an aqueous phase were also controlled by placing surface-active magnetic NPs at an aqueous−air interface, which interacted with either or both kinds of interacting NPs in an aqueous phase and reduced their ability to interact with each other. On the other hand, water-soluble zwitterionic magnetic NPs proved to be excellent extractants of both Au and Ag NPs from the aqueous phase. Extraction efficiency depended on the strength of interactions between the water-soluble magnetic NPs and aqueous-solubilized Au and/or Ag NPs.

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Section: Resultssupporting

confidence: 53%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Colloidal Stabilization of Sodium Dilauraminocystine for Selective Nanoparticle–Nanoparticle Interactions: Their Screening and Extraction by Iron Oxide Magnetic Nanoparticles

Kaur¹,

Sandhu²,

Khullar³

et al. 2021

Langmuir

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“…This continuous interest results from their unique physicochemical properties, which can be modified by adjusting their structural elements, namely the polar headgroups, hydrocarbon tails and covalent spacer [20][21][22]. Cationic gemini surfactants, in particular, have been employed with the goal of attaining more effectiveness for gene delivery [23][24][25][26][27][28][29][30][31][32][33]. Most reports have focused on compounds with quaternary ammonium headgroups and linear aliphatic tails, called bis-quats [31,33].…”

Section: Introductionmentioning

confidence: 99%

“…Cationic gemini surfactants, in particular, have been employed with the goal of attaining more effectiveness for gene delivery [23][24][25][26][27][28][29][30][31][32][33]. Most reports have focused on compounds with quaternary ammonium headgroups and linear aliphatic tails, called bis-quats [31,33]. However, these surfactants exhibit relatively high levels of cytotoxicity, which limit their use in biomedical applications [34].…”

Section: Introductionmentioning

confidence: 99%

Effective cytocompatible nanovectors based on serine-derived gemini surfactants and monoolein for small interfering RNA delivery

Costa

Oliveira

Silva

et al. 2021

Journal of Colloid and Interface Science

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“…Such system provides a simplistic model for exosome-like vesicles embedded in ECM and allows to study the interactions of these vesicles with other biologics and cells. 14 ,N 14 -tetramethyl-N 1 ,N 14 -ditetradecyltetradecane-1,14-diaminium dibromide (GS14) was a generous gift from Prof. Luis García-Río's Group (USC) and synthesized as described elsewhere [34]. Gold-coated AT-cut quartz crystals were bought from AWSensors (Spain).…”

Section: Introductionmentioning

confidence: 99%

Liposomes Embedded in Layer by Layer Constructs as Simplistic Exosome Transfer Model

Prieto¹,

Domínguez-Arca²,

Costa³

et al. 2020

Preprint

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Background. Exosomes are extracellular vesicles originating from the exfoliation of the cellular membrane. They are involved in cell-to-cell and cell-to-matrix signaling, exchange of bioactive molecules, tumorigenesis and metastasis, among others. To mitigate the limited understanding of exosome transfer phenomena, we developed a simplistic model that mimics exosomes and their interactions with cells and the extracellular matrix. The proposed model is a layer-by-layer (LbL) film built from the polycationic poly-L-lysine (PLL) and the glycosaminoglycan hyaluronic acid (HA) to provide ECM mimicry. Positively charged 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and N1,N1,N14,N14-tetramethyl-N1,N14-ditetradecyltetradecane-1,14-diaminium dibromide (GS14) liposomes were embedded in this construct to act as exosome analogs.Results. To simulate exosomes carrying substances, Nile Red was loaded as a model of lipophilic cargo molecules. The integration of each component was followed by quartz-crystal microbalance measurements, which confirmed the immobilization of intact liposomes on the underlying (PLL/HA)3 soft film. The release of Nile Red from liposomes either embedded in the LbL construct or exposed at its surface revealed a fast first order release. This system was validated as a model for exosome/cell interactions by incubation with breast cancer cells MDA-MB-231. We observed higher internalization for embedded liposomes when compared with surface-exposed ones, showcasing that the ECM mimic layers do not constitute a barrier to liposome/cell interactions but favor them.Conclusions. Our findings indicate that the developed model enhances the structural stability of liposomes and induces endocytosis from breast cancer cells. We envisage that the internalization can be tuned by exploring different levels of embedment to achieve a cellular uptake modulated in a spatiotemporal dependent manner. The versatility provided by the LbL technique will allow incorporating additional specialized biomaterials to better mimic the structure and composition of exosomes and their role in cell-to-cell communications.

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Bis-quaternary ammonium gemini surfactants for gene therapy: Effects of the spacer hydrophobicity on the DNA complexation and biological activity

Cited by 14 publications

References 42 publications

Colloidal Stabilization of Sodium Dilauraminocystine for Selective Nanoparticle–Nanoparticle Interactions: Their Screening and Extraction by Iron Oxide Magnetic Nanoparticles

Colloidal Stabilization of Sodium Dilauraminocystine for Selective Nanoparticle–Nanoparticle Interactions: Their Screening and Extraction by Iron Oxide Magnetic Nanoparticles

Effective cytocompatible nanovectors based on serine-derived gemini surfactants and monoolein for small interfering RNA delivery

Liposomes Embedded in Layer by Layer Constructs as Simplistic Exosome Transfer Model

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