Birth After the Reimplantation of a Human Embryo

Steptoe, P. C.; Edwards, Robert G.

doi:10.1016/s0140-6736(78)92957-4

Cited by 2,006 publications

(919 citation statements)

References 0 publications

Supporting

Mentioning

822

Contrasting

Unclassified

Order By: Relevance

“…Progress in the field of assisted reproduction and micromanipulation has been remarkable since the first report of a successful delivery from IVF in 1978 [49]. IVF is a complex process and involves multiple steps resulting in the fertilization of oocytes in the laboratory.…”

Section: Introductionmentioning

confidence: 99%

Effect of sperm entry on blastocyst development after in vitro fertilization and intracytoplasmic sperm injection — mouse model

Piotrowska-Nitsche

Chan

2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To investigate whether in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), influence the embryo's development and its quality using the mouse as a model. Methods Assisted fertilization was performed using ICSI and IVF. Fluorescent beads were adhered to the fertilization cone or place of previous sperm injection in the natural mated (NM), IVF and ICSI embryos, respectively. Embryo examination was carried out at the two-cell and blastocyst stage to determine the position of fluorescent bead. Protein expression was detected by fluorescence immunocytochemical staining and confocal microscopic imaging of blastocysts. Results IVF and ICSI embryos developed at rates comparable to NM group. Embryos show similar expression patterns of two transcription factors, Oct4 and Cdx2. The most preferred place for spermatozoa attachment was the equatorial site of the egg, whether fertilization occurred in vitro or under natural conditions. We also link the sperm entry position (SEP) to embryo morphology and the number of cells at the blastocyst stage, with no influence of the method of fertilization. Conclusions IVF and ICSI, do not compromise in vitro preimplantation development. Additional data, related to sperm entry, could offer further criteria to predict embryos that will implant successfully. Based on embryo morphology, developmental rate and protein expression level of key transcription factors, our results support the view that ART techniques, such as IVF and ICSI, do not perturb embryonic development or quality.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of sperm entry on blastocyst development after in vitro fertilization and intracytoplasmic sperm injection — mouse model

Piotrowska-Nitsche

Chan

2012

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…An association between embryos' cleavage rate and morphologic characteristics, and their ability to implant has been reported soon after the first live birth using IVF, and development of controlled ovarian hyperstimulation protocols [3][4][5]. Since then, a multitude of morphologic parameters associated with increased viability of pronuclear, cleavage stage, and blastocyst stage embryos were identified, and numerous embryo grading systems have been developed [6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Receiver operating characteristic (ROC) analysis of day 5 morphology grading and metabolomic Viability Score on predicting implantation outcome

Seli

Bruce

Botros³

et al. 2010

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose Assessment of embryo viability is a key component of in vitro fertilization (IVF) and currently relies largely on embryo morphology and cleavage rate. In this study, we used receiver operating characteristic (ROC) analysis to compare the Viability Score (generated by metabolomic profiling of spent embryo culture media using near infrared (NIR) spectroscopy) to morphologic grading for predicting pregnancy in women undergoing single embryo transfer (SET) on day 5. Methods A total of 198 spent embryo culture media samples were collected in four IVF centers located in the USA, Europe and Australia. First, 137 samples (training set) were analyzed by NIR to develop an algorithm that generates a Viability Score predictive of pregnancy for each sample. Next, 61 samples (validation set) were analyzed by observers blinded to embryo morphology and IVF outcome, using the Day 5 algorithm generated with the training set. Pregnancy was defined as fetal cardiac activity (FCA) at 12 weeks of gestation. Genet (2011) 28:137-144 DOI 10.1007 potential to be a better classifier for pregnancy outcome than morphology alone in women undergoing SET on day 5.

show abstract

“…2 Embryo donation families: Follow-up Since the birth of the first IVF baby nearly 30 years ago (Steptoe & Edwards, 1978), the number of couples using assisted reproductive techniques (ARTs) has steadily increased. By 2002, approximately one in every 100 babies born in the US was conceived through ARTs (American Society for Reproductive Medicine, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…However, higher emotional over-involvement of embryo donation parents was found, along with more reluctance to disclose the method of family creation. These preliminary findings are discussed in terms of implications regarding the importance of genetic and gestational relationships between parents and children.2 Embryo donation families: Follow-up Since the birth of the first IVF baby nearly 30 years ago (Steptoe & Edwards, 1978), the number of couples using assisted reproductive techniques (ARTs) has steadily increased. By 2002, approximately one in every 100 babies born in the US was conceived through ARTs (American Society for Reproductive Medicine, 2008).…”

mentioning

confidence: 99%

Embryo donation families: A follow-up in middle childhood.

MacCallum

Keeley

2008

Journal of Family Psychology

View full text Add to dashboard Cite

Couples who conceive using donated embryos rear a child to whom they are genetically unrelated. It has been suggested that this may have negative consequences for parenting and child development. Findings are presented of the second phase of an exploratory study of families with a child conceived through embryo donation. Seventeen embryo donation families with a 5-9 year old child were compared with 24 adoptive families and 28 in vitro fertilisation families. The quality of the mother's parenting, and the child's social and emotional development were assessed using standardised interviews and questionnaires administered to mothers and teachers. Embryo donation children were not at increased risk of psychological problems during middle childhood and the families were generally functioning well. However, higher emotional over-involvement of embryo donation parents was found, along with more reluctance to disclose the method of family creation. These preliminary findings are discussed in terms of implications regarding the importance of genetic and gestational relationships between parents and children.2 Embryo donation families: Follow-up Since the birth of the first IVF baby nearly 30 years ago (Steptoe & Edwards, 1978), the number of couples using assisted reproductive techniques (ARTs) has steadily increased. By 2002, approximately one in every 100 babies born in the US was conceived through ARTs (American Society for Reproductive Medicine, 2008). Furthermore, in the UK in 2004 nearly 30,000 couples attempted fertility treatment, a three-fold increase on the frequency in 1991 (HFEA, 2005). One form of ART is embryo donation, where an embryo created by the gametes of one couple is donated to another couple who then rear the resulting child. Unlike straightforward IVF, embryo donation was developed more recently and is less frequently used, with an average of ~35 embryo donation children born per year in the UK over the last decade (HFEA, personal communication). Concerns have been raised about the potentially adverse psychological consequences of this new method of family formation, but very little research has investigated the experiences of embryo donation families.Conceiving a child using donated embryos results in a family structure where the child is not genetically related to either parent, paralleling that seen in adoptive families.Adoption presents children with specific psychological tasks including coping with the knowledge that they are adopted, and incorporating this awareness into a coherent sense of identity (Triseliotis, Shireman, & Hundleby, 1997). Outcomes for the psychological development of adopted children are mixed. Non-clinical studies of adopted children do not find them to be at risk of problems with temperament or development in infancy and early childhood (Carey, Lipton, & Myers, 1974;Plomin & DeFries, 1985). However, in middle to late childhood, some adoptees exhibit higher levels of psychological maladjustment than nonadoptees (Brand & Brinich, 1999). One possible explanati...

show abstract

Birth After the Reimplantation of a Human Embryo

Cited by 2,006 publications

References 0 publications

Effect of sperm entry on blastocyst development after in vitro fertilization and intracytoplasmic sperm injection — mouse model

Effect of sperm entry on blastocyst development after in vitro fertilization and intracytoplasmic sperm injection — mouse model

Receiver operating characteristic (ROC) analysis of day 5 morphology grading and metabolomic Viability Score on predicting implantation outcome

Embryo donation families: A follow-up in middle childhood.

Contact Info

Product

Resources

About