Birefringence characteristics in sperm heads allow for the selection of reacted spermatozoa for intracytoplasmic sperm injection

Gianaroli, Luca; Magli, Maria Cristina; Ferraretti, Anna Pia; Crippa, Andor; Lappi, Michela; Capitani, Serena; Baccetti, Baccio

doi:10.1016/j.fertnstert.2008.10.024

Cited by 105 publications

(92 citation statements)

References 19 publications

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“…It has been hypothesized that microinjection of acrosome reacted sperm during ICSI would improve the outcomes of this technique since it mimics more closely the natural phenomenon of fertilization. Clinical data collected so far support this hypothesis, as pregnancy rates originated from embryos produced with acrosomereacted spermatozoa were significantly higher compared with those in the control group (ICSI with non-reacted spermatozoa) (Gianaroli et al, 2010).…”

Section: Selection Based On Live Sperm Morphologymentioning

confidence: 72%

Methods for Sperm Selection for In Vitro Fertilization

Ortega¹,

Bosch²

2012

In Vitro Fertilization - Innovative Clinical and Laboratory Aspects

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Section: Selection Based On Live Sperm Morphologymentioning

confidence: 72%

Methods for Sperm Selection for In Vitro Fertilization

Ortega¹,

Bosch²

2012

In Vitro Fertilization - Innovative Clinical and Laboratory Aspects

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“…This birefringence is associated with sub-acrosomal protein filaments and nucleoprotein filaments ( Figure 2). The presence of birefringence in the head indicates the good morphology of a spermatozoon [26][27][28][29][30][31].…”

Section: Polarized Light Microscopymentioning

confidence: 99%

“…Two types of head birefringence are ascertained on the basis of acrosome integrity: (1) partial head birefringence in acrosome-reacted spermatozoa and (2) total head birefringence in acrosome-non-reacted spermatozoa. Using acrosome-reacted spermatozoa shows better ICSI results [28][29][30]. Spermatozoa with partial head birefringence can present lower ratio of DNA fragmentation and higher ratio of normal nucleus [29].…”

Section: Spermatozoa -Facts and Perspectivesmentioning

confidence: 99%

Assessment of Human Sperm Cells Morphological Parameters

Lasienë¹

2018

Spermatozoa - Facts and Perspectives

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The quality of spermatozoa has a direct influence on the fertilization and developmental competence of embryos. The aim of this work was to review the methods of spermatozoa morphology assessment, features of the normal spermatozoa and the reasons of their several abnormalities. Three methods can be used for the evaluation of spermatozoa morphology in the in vitro fertilization (IVF) laboratory: (1) light microscopy of stained spermatozoa, (2) motile sperm organelle morphology examination (MSOME) and (3) polarized light microscopy. The analysis of spermatozoa morphology includes the assessment of head, neck, midpiece and tail. Morphologically abnormal spermatozoa are categorized into subgroups according to the defects of the head, neck, midpiece and/or tail. Before IVF and intracytoplasmic sperm injection (ICSI), the quality of spermatozoa must be estimated exactly, because this has the high influence on embryo development. Therefore the analysis of the morphological parameters of spermatozoa using the light microscopy, MSOME, in combination with precise head birefringence detection using the polarized microscopy, could give the best fertilization rate and embryo quality after IVF and ICSI.

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“…Used in ICSI, this technique seems to improve implantation and clinical pregnancy rates (Gianaroli et al, 2008;Gianaroli et al, 2010). Sperm selection based on its ability to recognize and bind hyaluronic acid (HA), a major component of cumulus-oocyte complexes extracellular matrix, has been also proposed.…”

Section: Impact Of Sperm Dna Damages On Fertility and Artmentioning

confidence: 99%