Bipolar Membrane Electroacidification To Produce Bovine Milk Casein Isolate

Abstract

Bipolar membrane electroacidification (BMEA) has been developed previously (Bazinet et al., Report for the Canadian Electricity Association 9326 U 987, 1996; Bazinet et al., J. Agric. Food Chem. 1997, 45, 2419-2425, 3788-3794) and has been used for isoelectric precipitation of soybean proteins. The purpose of this study was to validate the feasibility of BMEA for the precipitation of milk casein and to investigate the effect of flow rate. High-purity isolates containing 1.23 and 2.00% ash and 85.4 and 91.6% total protein were obtained with flow rates of 0.2 and 1.2 gal/min. The molecular composition profiles of the isolates obtained by HPLC showed that only caseins were precipitated. However, except for protein precipitation curves, the flow rate did not influence the final composition and purity of the isolates. These results showed that BMEA is a new alternative process for the production of high-purity bovine milk casein isolate.

Identification of Skim Milk Electroacidification Fouling: A Microscopic Approach

“…1). This deposit affected the performance of the system and decreased the life-time of the membrane (10). In a previous work on membrane permselectivity, this deposit was identified as a mix of calcium and magnesium salts (11).…”

“…This procedure was used to produce high-purity bovine milk casein isolates (10). However, a deposit was observed on the CEM side in contact with the base (Fig.…”

Fractionation of whey proteins by bipolar membrane electroacidification

“…This procedure was recently used to produce high purity bovine milk casein (Bazinet, Lamarche, Ippersiel & Amiot, 1999a) and soybean isolates (Bazinet et al, 1997a) and to fractionate soybean 11S and 7S fractions (Bazinet, Ippersiel, Labrecque & Lamarche, 2000a).…”

The Application of Membrane Separation Technology in the Dairy Industry

“…Laurent et al [67] used electrodialysis for demineralization of skim milk with rate up to 75%. This is much better than their previous study (30-40% demineralization rate) [68]. Chen et al [69] also successfully applied electrodialysis to remove the lactate ions from acid whey in order to solve operational problems in downstream spray drying operations.…”