Biphasic responses of human vascular smooth muscle cells to magnesium ion

Ma, Jun; Zhao, Nan; Zhu, Donghui

doi:10.1002/jbm.a.35570

Cited by 70 publications

(53 citation statements)

References 56 publications

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“…Understanding the cellular behaviors of endothelial cells and SMCs exposed to surrounding microenvironment are critical in stent application2527. Intense policy regulation on implantable medical device would require such information to be fully disclosed for any Zn-based device.…”

Section: Discussionmentioning

confidence: 99%

Bioabsorbable zinc ion induced biphasic cellular responses in vascular smooth muscle cells

Zhao

Zhu

2016

Sci Rep

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Bioabsorbable metal zinc (Zn) is a promising new generation of implantable scaffold for cardiovascular and orthopedic applications. In cardiovascular stent applications, zinc ion (Zn2+) will be gradually released into the surrounding vascular tissues from such Zn-containing scaffolds after implantation. However, the interactions between vascular cells and Zn2+ are still largely unknown. We explored the short-term effects of extracellular Zn2+ on human smooth muscle cells (SMCs) up to 24 h, and an interesting biphasic effect of Zn2+ was observed. Lower concentrations (<80 μM) of Zn2+ had no adverse effects on cell viability but promoted cell adhesion, cell spreading, cell proliferation, cell migration, and enhanced the expression of F-actin and vinculin. Cells treated with such lower concentrations of Zn2+ displayed an elongated shape compared to controls without any treatment. In contrast, cells treated with higher Zn2+ concentrations (80–120 μM) had opposite cellular responses and behaviors. Gene expression profiles revealed that the most affected functional genes were related to angiogenesis, inflammation, cell adhesion, vessel tone, and platelet aggregation. Results indicated that Zn has interesting concentration-dependent biphasic effects on SMCs with low concentrations being beneficial to cellular functions.

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Section: Discussionmentioning

confidence: 99%

Bioabsorbable zinc ion induced biphasic cellular responses in vascular smooth muscle cells

Zhao

Zhu

2016

Sci Rep

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“…In the cardiovascular system, one of the main mechanisms that regulates VSMC activity during pressure load is the Ca channel block mediated by Mg [7–14]. This implies that Mg depletion is associated with a high risk of cardiovascular disease (CVD) [2] but little is known about the Mg/Ca concentrations in acute aortic dissection (AAD), a dramatic cardiovascular disorder that is often fatal because of late diagnosis [15].…”

Section: Introductionmentioning

confidence: 99%

A pilot observational study on magnesium and calcium imbalance in elderly patients with acute aortic dissection

et al. 2017

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BackgroundMagnesium (Mg) and calcium (Ca) are the principal essential elements involved in endothelial cell homeostasis. Extracellular changes in the levels of either alter endothelial contraction and dilatation. Consequently Mg and Ca imbalance is associated with a high risk of endothelial dysfunction, the main process observed during acute aortic dissection (AAD); in this clinical condition, which mainly affects elderly men, smooth muscle cell alterations lead to intimal tears, creating a false new lumen in the media of the aorta. AAD patients have a high risk of mortality as a result of late diagnosis because often it is not distinguished from other cardiovascular diseases.We investigated Mg and Ca total circulating levels and the associated pro-inflammatory mediators in elderly AAD patients, to gain further information on the pathophysiology of this disorder, with a view to suggesting newer and earlier potential biomarkers of AAD.ResultsTotal circulating Mg and Ca levels were both lower in AAD patients than controls (p < 0.0001). Using Ca as cut-off, 90% of AAD patients with low Ca (<8.4 mg/dL) came into the type A classification of AAD. Stratifying AAD according to this cut-off, Mg was lower in patients with lower total Ca.Compared to controls, both type A and B AAD patients had higher levels of all the pro-coagulant and pro-inflammatory mediators analyzed, including sP-sel, D-dimer, TNF-α, IL-6, and CRP (p < 0.05). Dividing types A and B using the Stanford classification, no significant differences were found (p > 0.05) The levels of both ICAM-1 and EN-1 were lower in AAD than in a control group (p < 0.0001 and p < 0.05 respectively).ConclusionsThese findings suggest that low Mg and Ca in AAD elderly patients may contribute to altering normal endothelial physiology and also concur in changing the normal concentrations of different mediators involved in vasodilatation and constriction, associated with AAD onset and severity.

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“…It is speculated that the degradation products of Mg 2+ and OH − were the major factors to inhibit the cell proliferation. Numerous studies revealed that excessive Mg 2+ and OH − would induce cell damage and apoptosis . Although it had a decreased proliferation rate for MSCs on Mg metal, the cell viability was elevated by Mg metal after UV radiation.…”

Section: Discussionmentioning

confidence: 99%

Effect of magnesium on reducing the UV‐induced oxidative damage in marrow mesenchymal stem cells

Chen

Xiong

Zhao

et al. 2019

J Biomedical Materials Res

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Oxidative stress could cause damage to lipids, proteins and DNA, which is induced by the imbalance between the production of reactive oxygen species (ROS) and the biological system ability to counteract or detoxify their harmful effects. The oxidative stress damage significantly contributes to a number of diseases. Magnesium (Mg) is endowed with a novel function of removing excess ROS by releasing H2 during the degradation. In this study, in order to explore the property of anti‐oxidative damage of Mg metal, rat bone marrow mesenchymal stem cells (MSCs) oxidative damaged by ultraviolet (UV) radiation was employed to co‐culture with Mg metal. The effect of Mg metal on the response of antioxidant enzymes and mitochondria in MSCs was studied. We found that Mg metal could reduce the cellular oxidative stress damage and elevate the activities of antioxidant enzymes to maintain redox homeostasis. In addition, Mg metal could reduce the risk of UV‐induced cell apoptosis by increasing the ratio of Bcl‐2/Bax, elevating the mitochondrial membrane potential and blocking the release of cytochrome c. This finding showed Mg metal might have the potential for treating diseases caused by oxidative stress damage. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1253–1263, 2019.

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Biphasic responses of human vascular smooth muscle cells to magnesium ion

Cited by 70 publications

References 56 publications

Bioabsorbable zinc ion induced biphasic cellular responses in vascular smooth muscle cells

Bioabsorbable zinc ion induced biphasic cellular responses in vascular smooth muscle cells

A pilot observational study on magnesium and calcium imbalance in elderly patients with acute aortic dissection

Effect of magnesium on reducing the UV‐induced oxidative damage in marrow mesenchymal stem cells

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