Biphasic response against aeroallergen in atopic dermatitis showing a switch from an initial TH2 response to a TH1 response in situ: An immunocytochemical study

Thepen, Theo; Langeveld-Wildschut, Elisabeth G.; Bihari, Ilse C.; Wichen, D.F. Van; Reijsen, Frank C. van; Mudde, Geert C.; Bruijnzeel-Koomen, Carla A.F.M.

doi:10.1016/s0091-6749(96)80161-8

Cited by 379 publications

(262 citation statements)

References 30 publications

Supporting

Mentioning

239

Contrasting

Unclassified

Order By: Relevance

“…Whereas in acute AD lesions a predominance of Th2-producing cells has been observed, a shift to a Th1 cytokine pattern has been described in chronic lesions (9). Although IFN-␥ has been shown to be produced in both acute and chronic skin lesions in humans (10,11), high numbers of CD4 ϩ and CD8 ϩ T cells expressing IFN-␥ infiltrate acute AD lesions (12). Moreover, a significant proportion of house dust mite-specific T cell clones isolated from AD lesional skin were of a CD8 phenotype (13).…”

Section: Skin-infiltrating Cd8mentioning

confidence: 99%

Skin-Infiltrating CD8+ T Cells Initiate Atopic Dermatitis Lesions

Hennino

Vocanson

Toussaint

et al. 2007

The Journal of Immunology

103

View full text Add to dashboard Cite

Skin lesions in the allergic form of atopic dermatitis (AD) are induced by allergen-specific T cells that infiltrate the skin at the site of allergen exposure. Although Th2-type CD4+ T cells appear to be crucial in AD pathophysiology, little is known about the contribution of CD8+ T cells in the development of the allergic skin inflammation. In the present study, we have analyzed the respective role of CD8+ and CD4+ T cells in the development of AD skin lesions in a mouse model of allergen-induced AD. In sensitized mice, CD8+ T cells are rapidly and transiently recruited to the allergen-exposed site and initiate the inflammatory process leading to skin infiltration with eosinophils and Th1/Th2-producing cells. CD8+ T cell-depleted mice show no inflammation, demonstrating that these cells are mandatory for the development of AD. In contrast, CD4+ T cell-depleted mice develop a severe form of eczema. Furthermore, adoptive transfer of CD8+ T cells from sensitized mice into naive recipient mice leads to skin inflammation soon after allergen exposure. These data indicate that allergen-primed CD8+ T cells are required for the development of AD-like lesions in mice.

show abstract

Section: Skin-infiltrating Cd8mentioning

confidence: 99%

Skin-Infiltrating CD8+ T Cells Initiate Atopic Dermatitis Lesions

Hennino

Vocanson

Toussaint

et al. 2007

The Journal of Immunology

103

View full text Add to dashboard Cite

show abstract

“…Persistence of M1 macrophages in the local inflammatory response can also prevent the resolution of inflammation in several chronic skin diseases, such as diabetes-associated skin ulcerations, 14 chronic venous ulcers, 15 and atopic dermatitis. 16 Targeting M1 macrophages during chronic inflammation could therefore be a promising intervention strategy to correct an imbalance between M1 and M2 macrophages, thus promoting the resolution of chronic inflammation. Although FcgRs are present in both human and mouse, there are several species-dependent differences.…”

Section: Introductionmentioning

confidence: 99%

Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies

Hristodorov¹,

Mladenov²,

Felbert³

et al. 2015

mAbs

View full text Add to dashboard Cite

(2015) Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies, mAbs, 7:5, 853-862, DOI: 10.1080/19420862.2015 To link to this article: https://doi.org/10. 1080/19420862.2015 (classical M1 or alternative M2), that play a differential role in immune regulation. In general, the M1 contribute to onset of inflammation, whereas the M2 orchestrate resolution and repair, whereby failure to switch from predominantly M1 to M2 reinforces a pro-inflammatory environment and chronic inflammation. Here, we show selective elimination of M1 macrophages in vitro by a range of CD64-targeted immunotoxins, including H22 (scFv)-ETA'. After re-polarization of already polarized macrophages, still only M1 polarization showed sensitivity toward CD64-directed immunotoxins. The selectivity for M1 was found linked to reduced endosomal protease activity in M1 macrophages as demonstrated by inhibition of endosomal proteases. Using the H22(scFv)-ETA' in a transgenic mouse model for chronic cutaneous inflammation, the M1 specificity was confirmed in vivo and a beneficial effect on inflammation demonstrated. Also ex vivo on skin biopsies from atopic dermatitis and diabetes type II patients with chronically-inflamed skin, a clear M1 specific effect was found. This indicates the potential relevance for human application. Our data show that targeting M1 macrophages through CD64 can be instrumental in developing novel intervention strategies for chronic inflammatory conditions.

show abstract

“…This decrease could be explained by the cell-mediated response in dogs with AD in our study. During the cell-mediated response, Th1-lymphocytes and macrophages predominate and this type of response has often been described in AD in association with chronic inflammation and the late-phase of an allergic response (Thepen et al 1996;de Vries et al 1998;Lee et al 2000). Exposure to stress which causes more serious alterations to the percentage of B-lymphocytes than T-lymphocytes (Dhabhar et al 1995) might play a role in the decrease of both the relative and absolute numbers of B-lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

“…The acute phase allergic response is characterised by Th2 lymphocytes, eosinophils and the pro-inflammatory cytokines IL-4, IL-5 and IL-13 (Thepen et al 1996;Lee et al 2000), whereas the chronic phase of AD is characterised by a predominance of Th1 lymphocytes, macrophages and IL-2, IL-12, IFN-γ and IL-18 cytokines (de Vries et al 1998). In human medicine, a recent popular hypothesis has posited that the resulting type of immune response in AD depends on the ratio of IL-10-to IFN-γ-or IL-4-secreting cells (Akdis et al 2004).…”

mentioning

confidence: 99%

Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis

Hoskova¹,

Svoboda²,

Satinska³

et al. 2015

Vet. Med. - Czech

View full text Add to dashboard Cite

Canine atopic dermatitis (CAD) is a chronic allergic disease characterised by genetic predisposition. The exact pathogenesis of the disease is still not fully understood. Blood parameters, lymphocyte subpopulations, cytokines and other molecules involved in pro-and anti-inflammatory activities, and Th1/Th2 polarisation in peripheral blood were assessed in clinically healthy dogs and dogs with AD by haematological examination, flow cytometry and qRT-PCR. The group of diseased dogs was further divided into subgroups according to the severity of clinical signs expressed as CADESI-03 (canine atopic dermatitis extent and severity index) values. The group of dogs with CAD showed mild neutrophilia without general leucocytosis, reduced numbers of B-lymphocytes (P < 0.05), increased relative numbers of T-lymphocytes (P < 0.01) decreased absolute numbers of double-positive lymphocytes (P < 0.001) and NK cells (P < 0.05) as compared with healthy controls. The expression levels of IL-10 mRNA were significantly higher (P < 0.05) in the group with CAD. Correlation with CADESI-03 was observed in expression levels of CCL3 (P < 0.05), CCL5 (P < 0.05) and SOCS-3 mRNAs (P < 0.05). In conclusion, the disease stage investigated in the present study is characterised by a chronic inflammatory response.

show abstract

Biphasic response against aeroallergen in atopic dermatitis showing a switch from an initial TH2 response to a TH1 response in situ: An immunocytochemical study

Cited by 379 publications

References 30 publications

Skin-Infiltrating CD8+ T Cells Initiate Atopic Dermatitis Lesions

Skin-Infiltrating CD8+ T Cells Initiate Atopic Dermatitis Lesions

Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies

Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis

Contact Info

Product

Resources

About