“…After showing that a protein complex formed by LAP1 and TRF2 occurs in vitro , we investigated the establishment of this interaction in human cells. We hypothesized that it could be constitutively active in the cell or, instead, be triggered by a specific signaling event, more precisely by DDR activation, given the previously reported inhibitory [ 33 , 34 , 35 , 36 , 37 , 38 , 39 ] and stimulatory [ 40 , 41 , 42 , 43 , 44 , 45 , 46 ] roles of TRF2 as well as the recently suggested involvement of LAP1 [ 29 ] in this biological process. Before directly addressing this question, cellular models of DNA damage induction were developed; these consisted in exposing HeLa cells to different concentrations of H 2 O 2 —a reactive oxygen species (ROS)—or bleomycin—a radiomimetic anticancer drug—for distinct time periods.…”