Biphasic fermentation strategy: an alternative to produce chlamydospores of nematophagous fungus<i>Duddingtonia flagrans</i>

Castillo-Saldarriaga, Carlos Rafael; Céspedes‐Gutiérrez, Elizabeth; Cubides-Cárdenas, Jaime; Álvarez, Martha Isabel Gómez; Márquez-Lara, Dildo

doi:10.1080/09583157.2020.1793908

Cited by 8 publications

(4 citation statements)

References 16 publications

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“…Our study demonstrated again the important role of chlamydospores in the cycle of the development of predacious fungi, as they are the most promising propagules for the production of biological preparations under submerged technology due to their ability to survive in soil for a long time, faster germination in soil and weaker sensitivity to soil fungistasis, stability to drying and low temperatures and continuous conservation, which, in its turn, facilitates processing, storing, and transporting (Teplyakova, 2019;Castillo-Saldarriaga C et al, 2020;Federica SM et al, 2013;Li et al, 2016).…”

Section: Discussionmentioning

confidence: 66%

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2021

Agric. sci. pract.

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Section: Discussionmentioning

confidence: 66%

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2021

Agric. sci. pract.

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“…Moreover, Santurio et al [24] reports the production of chlamydospores using rice without the addition of inducing substances or any additional carbon or nitrogen source with a value of 5x10 4 chlamydospores g dry substrate -1 day -1 . Castillo Saldarriaga et al [25] achieved a productivity of 7x10 5 chlamydospores g dry substrate -1 day -1 using split rice as substrate. Finally, Silva et al 6 attained a productivity of 2.5x10 5 chlamydospores g -1 dry substrate day -1 using rice grits as a fermentation substrate.…”

Section: Discussionmentioning

confidence: 99%

Chlamydospores production enhancement of Duddingtonia flagrans in a solid-state fermentation system

Osorio

Saldarriaga

Cardenas

et al. 2021

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Resistance structures such as chlamydospores produced by the fungus Duddingtonia flagrans allow the reduction of infectious larvae from gastrointestinal nematodes. The objective of this research was to study the effect of carbon and nitrogen sources on the production of chlamydospores of the fungus in a solid state fermentation system (SSF). Twelve substances were studied using a statistical strategy, evaluating their effect on the production of Chlamydospores Finally, using an optimization strategy, the modifications of the substances that favor the production of chlamydospores were defined, and the effect of these on the predatory capacity of the fungus was evaluated. Optimal conditions were the variability of 0.25% w / w ammonium sulfate and 0.56% w / w sodium acetate in broken rice. The maximum concentration reached under this condition was 2.27x107 chlamydospores g of dry substrate-1, with a productivity of 1.62x106 chlamydospores g of dry substrate-1 day-1.

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“…168 of 2017. For use in each experiment, the fungal isolate was produced following the solid‐state fermentation methodology described by Castillo‐Saldarriaga et al [15]. Briefly, 68 g of broken rice were placed in an aluminum tray (12 × 18 × 3.5 cm) and autoclaved (121°C for 40 min) with 87 g of water.…”

Section: Methodsmentioning

confidence: 99%

In vitro evaluation of physicochemical variables on the nematophagous fungus Duddingtonia flagrans

et al. 2021

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Duddingtonia flagrans is a biological alternative to the use of anthelmintic drugs in ruminants. This fungus must be ingested by the animal, pass through the cavities of the digestive tract and reach the feces where it develops traps that capture the nematodes. The severe conditions encountered in this process negatively affect the fungus, which is reflected in the low recovery rates compared to the amount administered. The aim of this study was to evaluate independently the in vitro effect of typical physical and chemical conditions of the gastrointestinal cavities of ruminants on the concentration, viability, and the in vitro nematode predatory ability of the chlamydospores of D. flagrans. The factors evaluated individually were pH (2, 6, and 8), temperature (28 ± 2°C and 39 ± 2°C), exposure to artificial saliva, and milling. The results showed that the concentration and viability of D. flagrans were not affected by the action of pH, temperature, milling, or exposure to artificial saliva. Regarding the in vitro nematode predatory ability, a reduction was observed after the milling process and the exposure for 24 h at different pH.

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Biphasic fermentation strategy: an alternative to produce chlamydospores of nematophagous fungusDuddingtonia flagrans

Cited by 8 publications

References 16 publications

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Chlamydospores production enhancement of Duddingtonia flagrans in a solid-state fermentation system

In vitro evaluation of physicochemical variables on the nematophagous fungus Duddingtonia flagrans

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