Biphasic effect of<i>Syzygium aromaticum</i>flower bud on reproductive physiology of male mice

Mishra, Raghav Kumar; Singh, Shilpi

doi:10.1111/and.12533

Cited by 15 publications

(13 citation statements)

References 27 publications

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“…Further, the present study indicates that Shilajit may stimulate testosterone biosynthesis by increasing the activity of testicular hydroxysteroid dehydrogenases. Pituitary gonadotropins are prime regulators of testicular androgenic enzymes activities; therefore, the increase in the activities of testicular Δ 5 3β‐HSD and 17β‐HSD may be due to the effect of Shilajit on pituitary gonadotropins secretion (Mishra & Singh, ; Verma & Singh, ). It is, therefore, reasonable to assume that Shilajit improves the functional status of male reproductive organs and the related endocrine pathways through pituitary–testicular axis, presumably by acting as an antioxidant; however, this needs further investigations.…”

Section: Discussionmentioning

confidence: 99%

“…In mice spermatogenesis, developing spermatids spend 4.84 days in step 14–16. Therefore, to calculate daily sperm production, the number of steps 14–16 spermatids was divided by 4.84 (Mishra & Singh, ).…”

Section: Methodsmentioning

confidence: 99%

“…The tissue was minced carefully in the medium with fine forceps and scissors to ensure the extrusion of spermatozoa. The tissue fraction was removed with the help of fine forceps and a needle, and the suspension was used for the assessment of motility and concentration of spermatozoa (Mishra & Singh, ; Patel, Singh, Singh, & Singh, ).…”

Section: Methodsmentioning

confidence: 99%

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Profertility effects of Shilajit on cadmium-induced infertility in male mice

2018

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Shilajit is claimed as a Vajikarak (aphrodisiac) and used for the treatment of male infertility by traditional healers of the Indian subcontinent. Therefore, the present investigation was designed to assess the effectiveness of Shilajit for treatment of male infertility resulting from exposure to perilous chemicals. Effect of daily oral administration (p.o.) of Shilajit (50 mg, 100 mg and 200 mg/Kg BW) was investigated for a single spermatogenic cycle (35 days) in cadmium-induced (2 mg/Kg BW, p.o. for 35 days) infertile adult (12-14 week) swiss male mice. Shilajit treatment increased weights of reproductive organs, testicular daily sperm production, activities of testicular Δ 3β-HSD and 17 β-HSD enzymes and serum level of testosterone. Histopathological evaluation of testis revealed that Shilajit restored spermatogenesis as reflected by a gradual augmentation in germ cell layers with increased doses of Shilajit compared to cadmium-treated mice. Further, Shilajit treatment reverted back the adverse effects of cadmium on motility and concentration of spermatozoa. Secretory activities of the epididymis and seminal vesicle and libido, fertility and the number of litters per female were also improved by Shilajit in cadmium-treated mice. Results thus suggest the potent androgenic nature of Shilajit and its role in fertility improvement against cadmium-induced infertility.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Profertility effects of Shilajit on cadmium-induced infertility in male mice

2018

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“…Testicular cholesterol concentration was determined by the enzymatic end‐point method using commercially available kits (BioAssay Systems) according to the protocol of the manufacturer. As used by Mishra and Singh (2016), the method of Talalay (1962) was used to measure the activity of 3β‐HSD, while the method of Jarabak, Adams, Williams‐Ashman, and Talalay (1962) was used to measure 17β‐HSD activity in the testis. In a total volume of 2 ml, the reaction mixture contained 100 μmol sodium pyrophosphate buffer (pH 8.9), 0.5 μmol NAD + for 3β‐HSD and NADP + for 17β‐HSD, 0.14 μmol dehydroepiandrosterone for 3β‐HSD and 0.5 μmol testosterone for 17β‐HSD, and 20 mg equivalent of microsomal protein as enzyme source.…”

Section: Methodsmentioning

confidence: 99%

Cyperus esculentusL. (tigernut) mitigates high salt diet‐associated testicular toxicity in Wistar rats by targeting testicular steroidogenesis, oxidative stress and inflammation

et al. 2020

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High salt diet (HSD) impairs testicular function via oxidative stress. Cyperus esculentus contains antioxidants and improves testicular function. We investigated the protective effect of hydro‐ethanolic extract of Cyperus esculentus on testicular function in HSD‐fed Wistar rats. Twenty‐five male Wistar rats (125–135 g) 8–9 weeks old were divided into five groups (n = 5): control, HSD‐fed (8 % NaCl in feed), extract‐treated (500 mg kg−1 day−1), HSD‐fed +500 mg kg−1 day−1 of extract and HSD‐fed +1,000 mg kg−1 day−1 of extract groups. Treatment lasted for 6 weeks. HSD decreased (p < .05) sperm parameters and serum reproductive hormones levels, while Cyperus esculentus extract improved (p < .05) sperm parameters, and serum testosterone and follicle‐stimulating hormone levels in HSD‐fed rats. The extract upregulated intra‐testicular testosterone level and activities of 3β‐hydroxysteroid dehydrogenase (3β‐HSD) and 17β‐HSD, downregulated malondialdehyde and nitric oxide levels, and exhibited a dose‐dependent decrease in pro‐inflammatory cytokines, upregulation of activities of enzymatic antioxidants and increase in total antioxidant capacity in testes of HSD‐fed rats. The extract at both doses improved Johnsen's score, Leydig and Sertoli cell counts and seminiferous tubular diameter in HSD‐fed rats. Cyperus esculentus exhibited a dose‐dependent mitigation of HSD‐associated testicular dysfunction by targeting testicular steroidogenesis, oxidative stress and inflammation.

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“…Cholesterol was assayed in the sample by the enzymatic end‐point method using a commercially available kit (Randox, UK) following the manufacturer's protocol. Testicular 3β‐HSD and 17β‐HSD activities were measured according to the methods of Talalay () and Jarabak, Adams, Williams‐Ashman, and Talalay (), respectively, as used by Mishra and Singh (). The reaction mixture in a total volume of 2 ml contained 100 μmol sodium pyrophosphate buffer (pH 8.9), 0.5 μmol NAD+ for 3β‐HSD and NADP+ for 17β‐HSD, 0.14 μmol dehydroepiandrosterone for 3β‐HSD and 0.5 μmol testosterone for 17β‐HSD, and 20 mg equivalent of microsomal protein as enzyme source.…”

Section: Methodsmentioning

confidence: 99%

Effect of quercetin on cadmium chloride-induced impairments in sexual behaviour and steroidogenesis in male Wistar rats

et al. 2017

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Cadmium chloride (CdCl ) has been reported to cause reproductive toxicity in male rats, mainly through oxidative stress. This study examined its effect on sexual behaviour, as one of the mechanisms of reproductive dysfunction, as well as the possible ameliorative effect of quercetin (QE) on same. Thirty male Wistar rats (10 weeks old), weighing 270-300 g, were used for this study. They were either orally administered 2% DMSO, CdCl (5 mg/kg b.w.), QE (20 mg/kg b.w.) or CdCl +QE, once daily for 4 weeks, before sexual behavioural studies. The 5th group received CdCl for 4 weeks and allowed 4-week recovery period, before sexual behavioural test. Rats were sacrificed after sexual behavioural studies. The blood, testis and penis were collected for biochemical assays. Cadmium increased mount, intromission and ejaculatory latencies, but reduced their frequencies, compared to control. Serum nitric oxide increased, while penile cyclic guanosine monophosphate reduced in the CdCl -exposed rats, compared to control. CdCl increased testicular cholesterol, but reduced 3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-HSD activities, and testosterone concentration. QE better attenuated these negative changes compared to withdrawal of CdCl treatment. In conclusion, CdCl suppressed steroidogenesis, penile erection and sexual behaviour, with poor reversal following withdrawal, while QE attenuated these effects.

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Biphasic effect ofSyzygium aromaticumflower bud on reproductive physiology of male mice

Cited by 15 publications

References 27 publications

Profertility effects of Shilajit on cadmium-induced infertility in male mice

Profertility effects of Shilajit on cadmium-induced infertility in male mice

Cyperus esculentusL. (tigernut) mitigates high salt diet‐associated testicular toxicity in Wistar rats by targeting testicular steroidogenesis, oxidative stress and inflammation

Effect of quercetin on cadmium chloride-induced impairments in sexual behaviour and steroidogenesis in male Wistar rats

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