Biphasic differential changes of GABAA receptor subunit mRNA levels in dentate gyrus granule cells following recurrent kindling-induced seizures

Expression of GABAA receptor subunit mRNAs in hippocampal pyramidal and granular neurons in the kindling model of epileptogenesis: an in situ hybridization study

“…Cortical levels of several subunits were long-lasting enhanced by seizure activity induced by/3-carboline kindling [32]. Kokaia et al [27] demonstrated a complex set of changes in the GABAAR mRNA levels after rapid kindling in the hippocampus (i.e. tetanic stimulation every 5 min).…”

“…During the first 4 hr after the last seizure all three subunits showed an initial decreased expression especially in the fascia dentata. Thereafter, up to 48 h, expression increased above control level and returned to control levels at 120 h. In CA1 and CA3 no overt changes were found [27]. Friedman et al investigated the effects of kainate induced seizures on al levels; 24 h after the seizures had started, a reduction of ~1 was found in CA3 and no changes in CA1 or fascia dentata [11].…”

“…Changes in subunit expression profile during development [30] and in response to transient cerebral ischemia [33], chronic GABAA/benzodiazepine receptor agonists/antagonists [15,25,42,56,63,80] or ethanol treatment [41,44,49] may underly functional changes of GABAARS. Modified GABAAR subunit mRNA levels were also described in rats that were subjected to repeated electrical stimulations with short interstimulus intervals resuiting in recurrent epileptiform seizures (rapid kindling procedure) [27].…”

Plasticity in GABAAReceptor Subunit mRNA Expression by Hypothalamic Magnocellular Neurons in the Adult Rat

“…To date, such studies have concentrated on developmental regulation, including changes during aging (MacLennan et al, 1991;Poulter et al, 1993;Fritschy et al, 1994;Gutierrez et al, 1994;Mathews et al, 1994), and examined the response of GABA A receptor expression and function in adults to a variety of allosteric GABA A receptor modulators (Kang and Miller, 1991;Montpied et al, 1991;Primus and Gallager, 1992;Mhatre et al, 1993;Tseng et al, 1994) as well as excitatory and inhibitory amino acids (Kim et al, 1993;Mhatre and Ticku, 1994;Zhu et al, 1995;Fénelon and Herbison, 1996). Further work has examined GABA A receptor expression in pathological states such as epilepsy (Kokaia et al, 1994;Kamphuis et al, 1995), Huntington's disease (Faull et al, 1993), and chronic stress (Montpied et al, 1993). Although these studies clearly have relevance in understanding the developmental and pathophysiological roles of GABA transmission in the brain, surprisingly little evidence exists for changes in GABA A receptor expression within a physiological context in the adult brain.…”

Two Types of Epilepsy Models and Processes of Cognition: Pentylenetetrazole Kindling and Absence Epilepsy of WAG/Rij Rats Strain

“…It is known that kindling can lead to a long-term decrease of the GABA A receptor complex density, due to changes in the synthesis of the respective proteins. A series of 40 kindlinginduced seizures (by rapid hippocampal stimulation) led to biphasic alterations of GABA A receptor subunit mRNA levels in dentate gyrus with only minor changes in CA 1 -CA 3 (Kokaia et al, 1994). Up to 4 hours after the last seizure the expression of mRNA for 1 subunit was slightly decreased in dentate gyrus, whereas marked reductions were observed for 3 and 2 subunits.…”