Biphasic action of retinoids on gonadotropin receptor induction in rat granulosa cells in vitro

Bagavandoss, P.; Midgley, A. Rees

doi:10.1016/0024-3205(88)90532-2

Cited by 26 publications

(16 citation statements)

References 36 publications

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“…RA inhibition has previously been reported to occur in granulosa cells in association with FSH-induced LH/human chorionic gonadotropin receptor induction as well as cAMP and progesterone accumulation (Bagavandoss & Midgley 1988). In this work, RA clearly suppressed the expression of LH receptor mRNA in granulosa cells matured by FSH, as revealed by RT-PCR analysis.…”

Section: Discussionsupporting

confidence: 70%

“…Thus, the differentiation of 3T3-L1 preadipocytes is inhibited in an early stage by RA (Chawla & Lazar 1994), whereas that in Ob17 and rat preadipocytes is strongly enhanced by RA (Safonova et al 1994). In rat ovarian granulosa cells, retinoids have been reported to exert a biphasic effect on luteinizing hormone (LH) receptor induction, as revealed by a ligand-binding assay (Bagavandoss & Midgley 1988), suggesting that the response of granulosa cells to RA depends upon the stage of differentiation.…”

Section: Introductionmentioning

confidence: 99%

“…A prominent biochemical event in this differentiation is the expression of LH receptor by the cAMP signaling pathway (Hsueh et al 1989). In a study using rat granulosa cells, RA inhibited LH receptor induction in a dose-dependent manner, and this inhibition was associated with a decrease in cAMP and progesterone levels (Bagavandoss & Midgley 1988). Despite these studies, however, the effects of RA on granulosa cell differentiation have not been adequately characterized.…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory effect of retinoic acid on the development of immature porcine granulosa cells to mature cells

Hattori

Takesue²,

Nishida³

et al. 2000

Journal of Molecular Endocrinology

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The present study investigated the effect of retinoic acid (RA) on the differentiation of granulosa cells prepared from porcine ovaries. The granulosa cells were precultured for 15 h, then cultured for 48 h with FSH and further treated for 24 h with LH in order to induce their transformation into luteal cells. After the cells had been exposed to 1 µM retinoids (RA, retinal and retinol) for 87 h, analysis of the LH receptor mRNA expression, an indicator of granulosa cell differentiation, was carried out by using semiquantitative RT-PCR. The results showed that there was a decrease in LH receptor mRNA levels, and that RA had a more potent effect on these levels than the other two retinoids. When cells were exposed to RA in the immature stage (before the addition of FSH) or the early stage of development (0-24 h after the addition of FSH), expression of LH receptor mRNA was greatly diminished. When the immature cells were cultured for 15 h with RA, then washed and cultured for 48 h with FSH and for 24 h with LH, the expression of LH receptor mRNA was not reversed. In the differentiated cells (24 h after the addition of FSH), however, RA no longer had any inhibitory effect. When the immature cells were exposed to RA, FSH-induced expression of c-fos mRNA was markedly decreased. In contrast, expression of c-jun and activating transcription factor-4 mRNAs remained constant. However, the expression of c-fos mRNA was not decreased by forskolin. The results indicate that RA is a potent inhibitor in the immature stage of porcine granulosa cell differentiation, probably through decreased expression of FSH receptor, but that RA does not inhibit differentiation in the mature stage of the cells.

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Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibitory effect of retinoic acid on the development of immature porcine granulosa cells to mature cells

Hattori

Takesue²,

Nishida³

et al. 2000

Journal of Molecular Endocrinology

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“…The ovarian response to FSH and LH can also be modulated by RA. In response to this retinoid, stimulation of steroidogenesis (Bugavandoss & Midgley 1988, Graves-Hoagland et al 1988, and restricted expression of FSH receptor (FSHr) in rat granulosa cells has been reported (Minegishi et al 1996(Minegishi et al , 2000, which could affect oocyte growth and in turn the outcome of OPU in the cow (Argov et al 2004). In the porcine, the effects are controversial, as exposure of immature granulosa cells to RA inhibited expression of LH receptor (LHr) by downregulation of c-Fos mRNA (Hattori et al 2000).…”

Section: Embryos Transferred Pregnancy (%)mentioning

confidence: 99%

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

Hidalgo¹,

Díez²,

Duque³

et al. 2005

Reproduction

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Retinoids have been shown to enhance developmental competence of the oocyte in cattle, sheep and pigs. In this study we investigated whether exogenous retinol stimulates the bovine oocyte during its intrafollicular growth and the time limits of exposure to exogenous retinol. In addition, we also determined the efficiency of ovum pick-up techniques in combination with retinol treatment and the viability of embryos after transfer to recipients. In Experiment 1, heifers were injected with retinol or vehicle, and concentrations of retinol in the blood were analysed on Day 0 (prior to injection), Day 1 and, together with follicular fluid, Day 4. Blood retinol increased by Day 1 and cleared on Day 4, but retinol remained higher within the follicle. In Experiment 2, oocyte donors were injected weekly with retinol or vehicle four times during a twice-per-week cycle of eight recovery sessions (starting 4 days before the first session), followed by a second eight-session cycle without treatment. Oocytes recovered were fertilized and culturedin vitro.Retinol treatment yielded higher numbers of low-quality oocytes throughout, although retinol measured during cycles did not change. Total oocytes, and morulae and blastocyst rates, increased during the first five sessions following treatment with retinol. As previously shown with oocytes from slaughterhouse ovaries, retinoic acid stimulated blastocyst development. Following transfer to recipients, blastocysts from oocytes exposed to retinol were unable to establish pregnancy. Our study confirms the existence of an effect of retinol on the intrafollicular oocyte in the cow and provides evidence regarding the teratogenic effect of retinol.

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“…Increased or maintained high levels of cAMP in oocytes prevent the meiosis resumption [27]. Bagavandoss and Midgeley [28] have suggested that RA may stimulate FSH for the induction of LH receptors and also encourage progesterone production and decrease cAMP levels. Cortical granule migration as a maturation clue could be affected by RA since RA improves granular migration [29,30].…”

Section: Discussionmentioning

confidence: 99%

Effects of retinoic acid on maturation of immature mouse oocytes in the presence and absence of a granulosa cell co-culture system

Tahaei

Eimani

Eftekhari‐Yazdi

et al. 2011

J Assist Reprod Genet

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Purpose Evaluation of the all-trans retinoic acid (t-RA) effects on in vitro maturation (IVM) and in vitro fertilization (IVF) of immature mouse oocytes in the presence and absence of granulosa cell monolayer. Methods Denuded oocytes isolated from mice ovaries and matured in IVM medium alone (Control I), IVM medium in the presence of granulosa cells (Control II), IVM medium with t-RA (Experimental I) and IVM medium simultaneously with t-RA and granulosa cells (Experimental II). After 24 h, matured oocytes were fertilized in T6 medium and their development was followed until the blastocyst stage. Metaphase II oocytes ploidy were evaluated by chromosome counting. Results The t-RA group compared to the control groups showed no obvious abnormalities. Additionally maturation and embryo development rates significantly increased in the t-RA treated granulosa cell co-culture system. Conclusions In conclusion, association of t-RA with granulosa cell co-culture during in vitro maturation increases meiosis resumption, formation of metaphase II oocytes, as well as 2-cell and blastocyst stage embryos.

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Biphasic action of retinoids on gonadotropin receptor induction in rat granulosa cells in vitro

Cited by 26 publications

References 36 publications

Inhibitory effect of retinoic acid on the development of immature porcine granulosa cells to mature cells

Inhibitory effect of retinoic acid on the development of immature porcine granulosa cells to mature cells

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

Effects of retinoic acid on maturation of immature mouse oocytes in the presence and absence of a granulosa cell co-culture system

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