Biovar-specific epitopes of the urease enzyme of Ureaplasma urealyticum

MacKenzie, Colin R.; Henrich, Birgit; Hadding, U.

doi:10.1099/00222615-45-5-366

Cited by 8 publications

(2 citation statements)

References 23 publications

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“…Clonal variants derived from single colonies of the corresponding strain. Additionally, the following U. urealyticum strains were used: serovar 9 (strain ATCC 33175), serovar 10 (strain Western CX3) and serovar 12 (strain JSL‐U24‐CX3) (MacKenzie et al ., 1996). Ureaplasma strains and clonal variants were cultivated in ureaplasma medium (UPM) as previously described (Zimmerman et al ., 2009).…”

Section: Methodsmentioning

confidence: 99%

Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172

Zimmerman¹,

Rosengarten

Spergser

2010

Molecular Microbiology

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Phase variation of the major ureaplasma surface membrane protein, the multiple-banded antigen (MBA), with its counterpart, the UU376 protein, was recently discussed as a result of DNA inversion occurring at specific inverted repeats. Two similar inverted repeats to the ones within the mba locus were found in the genome of Ureaplasma parvum serovar 3; one within the MBA N-terminal paralogue UU172 and another in the adjacent intergenic spacer region. In this report, we demonstrate on both genomic and protein level that DNA inversion at these inverted repeats leads to alternating expression between UU172 and the neighbouring conserved hypothetical ORF UU171. Sequence analysis of this phase-variable ‘UU172 element’ from both U. parvum and U. urealyticum strains revealed that it is highly conserved among both species and that it also includes the orthologue of UU144. A third inverted repeat region in UU144 is proposed to serve as an additional potential inversion site from which chimeric genes can evolve. Our results indicate that site-specific recombination events in the genome of U. parvum serovar 3 are dynamic and frequent, leading to a broad spectrum of antigenic variation by which the organism may evade host immune responses.

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Section: Methodsmentioning

confidence: 99%

Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172

Zimmerman¹,

Rosengarten

Spergser

2010

Molecular Microbiology

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“…urealyticum serotypes 3 and 8, representing the two different biovars [27], were grown in 1-L batches, harvested, and washed as previously described [28]. The final ureaplasma pellet was resuspended in 5 ml Pleuropneumonialike organism broth (Difco, Detroit, MI) pH 6.5 containing 5% dimethyl sulfoxide (Sigma, St. Louis, MO).…”

Section: Preparation Of U Urealyticum Specimensmentioning

confidence: 99%

In Vivo and In Vitro Impairment of Human and Ram Sperm Nuclear Chromatin Integrity by Sexually Transmitted Ureaplasma urealyticum Infection1

Reichart

Kahane

Bartoov

2000

Biology of Reproduction

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The incidence of Ureaplasma urealyticum infection in the semen of infertile men is variable (7%-42%). Evidence has accumulated through routine semen analysis to suggest that this infection can cause embryo loss without necessarily affecting sperm quality. The aim of this study was to specifically investigate the effects of U. urealyticum infection on sperm chromatin stability and DNA integrity, which are known to be correlated to pregnancy outcome. Sperm cells isolated from human semen infected in vivo with U. urealyticum exhibited a low percentage of stable chromatin as determined by nuclear chromatin decondensation assay (42% +/- 4.8%, n = 8) and a high percent of denatured DNA as determined by sperm chromatin structure assay (60.9% +/- 9.1%, n = 7). After doxycyclin treatment, a significant improvement in both parameters was observed (73.7% +/- 3.6%, P: < 0.001 and 30.1% +/- 3.5%, P: < 0.008, respectively). Sperm cells infected in vitro exhibited higher rates of viability and motility than uninfected cells. In contradistinction, U. urealyticum caused significant dose- and time-dependent chromatin decondensation and DNA damage. The percentage of human sperm cells with denatured DNA increased significantly by 54.9% +/- 23.9% and 47. 9% +/- 12.1%, after 30 min infection with serotypes 8 and 3, respectively, at a multiplicity of infection of 100 ureaplasmas per sperm compared with uninfected control cells. The damage to DNA was significantly more pronounced in infected ram sperm (180.9% +/- 21. 5%). These results indicate that preserved sperm activity post U. urealyticum infection resulted in damage to paternal DNA, although a high fertilization rate was maintained, and embryonic development may, therefore, be impaired.

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Ureaplasma

Robertson¹,

Taylor‐Robinson²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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U.re.a.plas'ma. N.L. fem. n. urea urea; Gr. neut. n. plasma anything formed or moulded, image, figure; N.L. neut. n. Ureaplasma urea form. Tenericutes / Mollicutes / Mycoplasmatales / Mycoplasmataceae / Ureaplasma Coccoid cells about 500 nm in diameter ; may appear as coccobacillary forms in exponential growth phase; filaments are rare. Nonmotile. Facultative anaerobes. Form exceptionally small colonies on solid media that are described either as tiny ( T ) “ fried‐egg ” colonies or as “ cauliflower head ” colonies having a lobed periphery. Unusual pH required for growth ( about 6 . 0–6 . 5 ). Optimal incubation temperature for examined species is 35–37°C. Chemo‐organotrophic. Like Mycoplasma , species of Ureaplasma lack oxygen‐dependent, NADH oxidase activity . Unlike Mycoplasma , species of Ureaplasma lack hexokinase or arginine deiminase activities but have a unique and obligate requirement for urea and produce potent ureases that hydrolyze urea to CO 2 and NH 3 for energy generation and growth. Genome sizes range from 760 to 1170 kbp (PFGE). Commensals or opportunistic pathogens in vertebrate hosts, primarily birds and mammals (mainly primates, ungulates, and carnivores). DNA G + C content ( mol %): 25–32 (Bd, T m ). Type species : Ureaplasma urealyticum Shepard, Lunceford, Ford, Purcell, Taylor‐Robinson, Razin and Black 1974, 167 emend. Robertson, Stemke, Davis, Harasawa, Thirkell, Kong, Shepard and Ford 2002, 593.

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Biovar-specific epitopes of the urease enzyme of Ureaplasma urealyticum

Cited by 8 publications

References 23 publications

Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172

Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172

In Vivo and In Vitro Impairment of Human and Ram Sperm Nuclear Chromatin Integrity by Sexually Transmitted Ureaplasma urealyticum Infection1

Ureaplasma

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