Biotypology of Yeast in Soils Polluted by Hydrocarbons in Fez-Meknes Region, Morocco

doi:10.26538/tjnpr/v5i9.13

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…In addition, Obuekwe et al, reported that yeasts can utilize n-alkanes as a sole energy and carbon source [ 12 , 13 ]. Many yeast species, such as Geotrichum sp., Candida lipolytica , Trichosporon mucoides and Yorrow lipolytica, isolated and purified from polluted water, were reported to their ability to degrade the petroleum oil pollutants [ 14 , 15 ]. Also, Benmessaoud et al, studied the diversity and biotypology of the yeasts in this region, which were used as a good indicator for the disturbance of the ecosystem by crude oil pollution [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Candida tropicalis B: a promising yeast strain for biodegradation of petroleum oil in marine environments

Hegazy,

Soliman,

Farag

et al. 2024

Microb Cell Fact

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The increasing interest in environmental protection laws has compelled companies to regulate the disposal of waste organic materials. Despite efforts to explore alternative energy sources, the world remains heavily dependent on crude petroleum oil and its derivatives. The expansion of the petroleum industry has significant implications for human and environmental well-being. Bioremediation, employing living microorganisms, presents a promising approach to mitigate the harmful effects of organic hydrocarbons derived from petroleum. This study aimed to isolate and purify local yeast strains from oil-contaminated marine water samples capable of aerobically degrading crude petroleum oils and utilizing them as sole carbon and energy sources. One yeast strain (isolate B) identified as Candida tropicalis demonstrated high potential for biodegrading petroleum oil in seawater. Physiological characterization revealed the strain’s ability to thrive across a wide pH range (4–11) with optimal growth at pH 4, as well as tolerate salt concentrations ranging from 1 to 12%. The presence of glucose and yeast extract in the growth medium significantly enhanced the strain's biomass formation and biodegradation capacity. Scanning electron microscopy indicated that the yeast cell diameter varied based on the medium composition, further emphasizing the importance of organic nitrogenous sources for initial growth. Furthermore, the yeast strain exhibited remarkable capabilities in degrading various aliphatic and aromatic hydrocarbons, with a notable preference for naphthalene and phenol at 500 and 1000 mg/l, naphthalene removal reached 97.4% and 98.6%, and phenol removal reached 79.48% and 52.79%, respectively. Optimization experiments using multi-factorial sequential designs highlighted the influential role of oil concentration on the bioremediation efficiency of Candida tropicalis strain B. Moreover, immobilized yeast cells on thin wood chips demonstrated enhanced crude oil degradation compared to thick wood chips, likely due to increased surface area for cell attachment. These findings contribute to our understanding of the potential of Candida tropicalis for petroleum oil bioremediation in marine environments, paving the way for sustainable approaches to address oil pollution.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Candida tropicalis B: a promising yeast strain for biodegradation of petroleum oil in marine environments

Hegazy,

Soliman,

Farag

et al. 2024

Microb Cell Fact

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Melanin antibacterial activity of two new strains, SN1 and SN2, of Exophiala phaeomuriformis against five human pathogens

Benmessaoud,

Anissi,

Kara

et al. 2023

Open Chemistry

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Melanin is an almost ubiquitous pigment of brown to dark black color synthesized by living organisms. It has excellent potential for application in several fields, especially in pharmaceuticals. Yeasts are known for their ability to synthesize pigments, including melanin. These yeasts are considered black yeasts. However, there is little information on the synthesis of melanin by these yeasts. This work was designed to study the ability of black yeast to produce a diffusible dark pigment on agar. Two strains of black yeast, SN1 and SN2, were isolated from hydrocarbon-polluted soils. Based on morphological, physiological, and molecular analyses, the two strains SN1 and SN2 were identified as new species of the Exophiala phaeomuriformis strain. After pigment extraction, melanin was characterized using UV and Fourier transform infrared spectroscopy. We found that melanin synthesis is strongly affected by light and optimal parameters such as temperature 30°C, salinity between 10 and 30 ppt, and basic pH. Both melanin extracts also showed inhibitory activity against pathogenic germs, and activities were observed on E. coli (33.33 ± 2.88 mm), Pseudomonas aeruginosa (29.33 ± 4.61 mm), and Staphylococcus aureus (24.3 ± 7.65 mm). It was concluded that the melanin of the E. phaeomuriformis strain isolated from oil-polluted soils had high antibacterial activity. This high antibacterial activity could be a suitable source of new antibacterial natural products.

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Isolation and Characterization of Three New Crude Oil Degrading Yeast Strains, Candida parapsilosis SK1, Rhodotorula mucilaginosa SK2 and SK3

et al. 2022

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Bioremediation using yeasts is an alternative way to minimize the effects of oil spillage on soil. This paper aims to establish a bioremediation protocol involving the optimization of physicochemical parameters. In this regard, three new yeast strains, SK1, SK2 and SK3, were isolated from hydrocarbon-contaminated samples from the Fez-Meknes region, Morocco. These isolates were identified as new species of Candida parapsilosis (SK1) and Rhodotorula mucilaginosa (SK2 and SK3), respectively, based on the similarity of their ITS region. The kinetic analysis of the process of degradation of petroleum oils are highlighted. These analyses were based on the degradation kinetics, and biomass formation using gravimetric analysis and gas chromatography coupled with mass spectrometry techniques. The strains were able to degrade 68% of the total petroleum hydrocarbon in 21 days, as the sole carbon source. The addition of glucose increased the rate at which crude oil was consumed by the isolates. Our results suggest that inoculants based on Candida parapsilosis (SK1) and Rhodotorula mucilaginosa (SK2 and SK3) cells have potential application in the biodegradation of crude oil and possibly in the degradation of other related aromatic compounds.

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Biotypology of Yeast in Soils Polluted by Hydrocarbons in Fez-Meknes Region, Morocco

Cited by 4 publications

References 32 publications

Isolation and characterization of Candida tropicalis B: a promising yeast strain for biodegradation of petroleum oil in marine environments

Isolation and characterization of Candida tropicalis B: a promising yeast strain for biodegradation of petroleum oil in marine environments

Melanin antibacterial activity of two new strains, SN1 and SN2, of Exophiala phaeomuriformis against five human pathogens

Isolation and Characterization of Three New Crude Oil Degrading Yeast Strains, Candida parapsilosis SK1, Rhodotorula mucilaginosa SK2 and SK3

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