Biotypes of Agrobacterium tumefaciens in Hungary

Süle, S.

doi:10.1111/j.1365-2672.1978.tb00792.x

Cited by 67 publications

(33 citation statements)

References 10 publications

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“…In addition, there are 2-3 genetically and phenotypically different groups in each species except for A. rubi7,9-11, 15,23,24), which are assigned to biovars 1, 2 and 313). This nomenclature at the species level does not, however, reflect the natural classification of Agrobacterium, because phytopathogenic behaviors used as taxonomic characters are not chromosomeencoded but plasmid-mediated13).…”

mentioning

confidence: 99%

Phenotypic Characteristics of the Genus Agrobacterium.

Sawada

Ieki

1992

Jpn. J. Phytopathol.

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confidence: 99%

Phenotypic Characteristics of the Genus Agrobacterium.

Sawada

Ieki

1992

Jpn. J. Phytopathol.

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“…It is well-known that biovar 3 strains have been frequently isolated from grapevine plants, and host range of many biovar 3 strains is relatively narrow when compared with those of biovars 1 and 2 (for a review, see Young et al, 2005). However, there are a few exceptions concerning the source host plants from which the biovar 3 strains were isolated (Sule, 1978;Bazzi and Rosciglione, 1982;Sawada and Ieki, 1992b). Among the eight biovar 3 strains which we used in this study ( Fig.…”

Section: Discussionmentioning

confidence: 98%

Construction of physical map and mapping of chromosomal virulence genes of the biovar 3 Agrobacterium (Rhizobium vitis) strain K-Ag-1

et al. 2006

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“…A fifth incomplete ORF (ttuE) encodes a pyruvate kinase which is induced by tartrate and required for optimal growth. Although the ttuABCD fragment allows growth of A. tumefaciens on tartrate, it does not provide full tartrate utilization in the original A. vitis background.In 1973, a new group of agrobacteria isolated from grapevines (Vitis vinifera) was described and classified as biotype III (17,32,46). Further studies demonstrated its close association with grapevine (4,5,23,33,34,42).…”

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Sequence and mutational analysis of a tartrate utilization operon from Agrobacterium vitis

Crouzet¹,

Otten²

1995

J Bacteriol

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The grapevine is the natural host of the tumorigenic bacterium Agrobacterium vitis. Most of the A. vitis isolates can use tartrate, an unusually abundant compound in grapevine. The nopaline strain, AB4, contains a 170-kb conjugative plasmid (pTrAB4) encoding tartrate utilization. A 5.65-kb pTrAB4 region which enables non-tartrate-utilizing Agrobacterium tumefaciens to grow on tartrate was sequenced and mutagenized with the transcriptional fusion transposon Tn5-uidA1. This DNA fragment contains four intact open reading frames (ORFs) (ttuABCD) required for tartrate-dependent growth. The mutant phenotypes of each ORF, their homologies to published sequences, and their induction patterns allowed us to propose a model for tartrate utilization in A. vitis. ttuA encodes a LysR-like transcriptional activator and is transcribed in the absence of tartrate. ttuB codes for a protein with homology to transporter proteins and is required for entry of tartrate into bacteria. ttuC codes for a tartrate dehydrogenase, while ttuD lacks homology to known sequences; the growth properties of ttuD mutants suggest that TtuD catalyzes the second step in tartrate degradation. A fifth incomplete ORF (ttuE) encodes a pyruvate kinase which is induced by tartrate and required for optimal growth. Although the ttuABCD fragment allows growth of A. tumefaciens on tartrate, it does not provide full tartrate utilization in the original A. vitis background.In 1973, a new group of agrobacteria isolated from grapevines (Vitis vinifera) was described and classified as biotype III (17,32,46). Further studies demonstrated its close association with grapevine (4,5,23,33,34,42). This Agrobacterium group is now considered as a separate species: Agrobacterium vitis (30). It is the only well-studied Agrobacterium group with a narrow natural host range.Part of the host specificity of A. vitis has been attributed to a chromosomally encoded polygalacturonase responsible for grapevine root necrosis (3,27,38 (17,33,47).In planta mating experiments (31, 48) have shown that in several A. vitis strains the tartrate degradation genes are carried by conjugative plasmids. The tartrate utilization plasmid of the A. vitis nopaline strain AB4 (pTrAB4) was chosen for further studies because of its relatively small size (170 kb).In Pseudomonas spp., biochemical studies have revealed two tartrate degradation pathways, one involving a dehydratase (1,14,20,45) and the other a tartrate dehydrogenase (TDH) and a reductase (7,18,25) or an L(ϩ)-tartrate decarboxylase (12). An L(ϩ)/D(ϩ)-tartrate dehydratase is also found in Salmonella typhimurium (54). The tartrate dehydratase gene of Escherichia coli is homologous to class I fumarase genes (35). TDH is part of the second pathway and has been isolated from Pseudomonas putida (19,49,50) and Rhodopseudomonas sphaeroides (11). P. putida TDH catalyzes the degradation of L(ϩ)-tartrate, meso-tartrate, and D(ϩ)-malate at the same active site by using NADH 2 and Mn 2ϩ (50). The P. putida tdh gene has recently been sequenced (49). So far, n...

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Biotypes of Agrobacterium tumefaciens in Hungary

Cited by 67 publications

References 10 publications

Phenotypic Characteristics of the Genus Agrobacterium.

Phenotypic Characteristics of the Genus Agrobacterium.

Construction of physical map and mapping of chromosomal virulence genes of the biovar 3 Agrobacterium (Rhizobium vitis) strain K-Ag-1

Sequence and mutational analysis of a tartrate utilization operon from Agrobacterium vitis

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