Th is study has two main approaches. First, it exploits the susceptibility of tomato cultivars as a prophylactic measure to detect auto resistance characters of the tested tomato varieties against Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae). Secondly, it evaluates the effi cacy of diff erent bio-rational insecticides against B. tabaci under greenhouse conditions. Th e results exhibited a special signifi cance in B. tabaci infestation suitability between the two tomato varieties with a high infestation signifi cance found in the Shifa F1 hybrid tomato variety compared to the Savera F1 hybrid tomato variety in the fi rst plantation period. Subsequently, in the second plantation period, there was a signifi cant diff erence between the two tomato varieties. Bemisia tabaci showed a preference for the Shifa F1 hybrid over the Savera F1 hybrid tomato variety. Th ese diff erences occurred during the 1st, 2nd, 4th, 6th, 7th, 8th, and 10th weeks. In the experimental trial for the effi cacy of eco-friendly biorational insecticides, spinosad, azadirachtin, Beauveria bassiana and Metarhizium anisopliae, there were signifi cant diff erences between the treated and untreated plants during the two plantation periods. A high effi cacy of spinosad on the B. tabaci population was found. Bemisia tabaci infestation under all the applications was reduced from 50 to 94.61% for the two plantation periods. Th is obvious decrease in B. tabaci population increase attention to benefi ts of the diff erent bio-rational insecticides.