Biotype stability of Candida albicans isolates after culture storage determined by randomly amplified polymorphic DNA and phenotypical methods

Bacelo, Kátia Leston; Costa, Karen Regina Carim da; Ferreira, Joseane Cristina; Candido, Renato

doi:10.1111/j.1439-0507.2009.01741.x

Cited by 16 publications

(18 citation statements)

References 37 publications

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“…When the DNA extraction cannot be immediately performed after collection and transportation, storage becomes an alternative. In this regard, studies have demonstrated that the DNA can be extracted after up to 180 days of storage for epidemiological purposes when C. albicans are stored in SDA (Bacelo et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Comparison of two storage conditions of Candida albicans for DNA extraction and analysis

Alves¹,

Ferrúa²,

Carvalho³

et al. 2015

Afr. J. Microbiol. Res.

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The major human fungal pathogens are species from the genus Candida, especially Candida albicans. C. albicans sample preservation from human source is essential for clinical purposes and epidemiologic studies; however, little is known about its conservation for DNA extraction and analysis. Therefore, the aim of this study was to evaluate and compare two storage conditions of C. albicans for performing DNA extraction and analysis. We collected samples from the intraoral palatal mucosa of patients with chronic atrophic candidiasis, and then assigned them into two groups for DNA extraction: Sabouraud dextrose agar (SDA) group samples taken from an SDA culture and the sterile buffering solution (SBS) group directly from the inoculum. We took C. albicans from samples stored in SBS and kept for two years at -80°C, and performed the DNA extraction with the Purgene DNA extraction kit for buccal cells. The means (standard deviation) for DNA extracted from C. albicans in SDA and SBS were, respectively, 16.62 ng/uL (10.53) and 9.732 ng/uL (2.342). In our qualitative evaluation, we observed no differences in band patterns between both treatment groups (SDA and SBS). The results show that SDA and SBS methods could preserve C. albicans' DNA for extraction to evaluate quantitative and qualitative data.

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Section: Discussionmentioning

confidence: 99%

Comparison of two storage conditions of Candida albicans for DNA extraction and analysis

Alves¹,

Ferrúa²,

Carvalho³

et al. 2015

Afr. J. Microbiol. Res.

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show abstract

“…C. albicans is an important cause of nosocomial infections; however, in the last decade, non- albicans species were responsible for an increasing number of infections (Bacelo et al 2010, Nawrot et al 2013). Yeasts are members of the normal microflora of the skin, mucous membranes and gastrointestinal tract in healthy individuals.…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland

Paluchowska

Tokarczyk

Bogusz³

et al. 2014

Mem. Inst. Oswaldo Cruz

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Over the last decades, Candida spp have been responsible for an increasing number of infections, especially in patients requiring intensive care. Knowledge of local epidemiology and analysis of the spread of these pathogens is important in understanding and controlling their transmission. The aim of this study was to evaluate the genetic diversity of 31 Candida albicans and 17 Candida glabrata isolates recovered from intensive care unit patients from the tertiary hospital in Krakow between 2011-2012. The strains were typed by random amplified polymorphic DNA (RAPD) polymerase chain reaction using five primers (CD16AS, HP1247, ERIC-2, OPE-3 and OPE-18). The results of the present investigation revealed a high degree of genetic diversity among the isolates. No clonal relationship was found among the C. albicans strains, whereas two C. glabrata isolates were identical. The source of Candida infection appeared to be mostly endogenous; however, the presence of two clonal C. glabrata strains suggested the possibility of cross-transmission of these pathogens. Our study confirmed the high discriminatory power of the RAPD technique in the molecular typing of Candida clinical isolates. This method may be applied to the evaluation of transmission routes of pathogenic fungi on a local level.

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“…On the other hand, the RAPD has been one of the most used for typing different microorganisms, such as Candida albicans (3,22).…”

Section: Fungi Of the Genusmentioning

confidence: 99%

Comparison of primers for RAPD-PCR from environmental isolates of Cryptococcus neoformans, Cryptococcus albidus and Cryptococcus laurentii complex

Pedroso¹,

Ferreira²,

Costa³

et al. 2012

Braz. J. Microbiol.

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Various organisms have been characterized by molecular methods, including fungi of the genusCryptococcus. The purposes of this study were: to determine the discriminatory potential of the RAPD (Random Amplified Polymorphic DNA) primers, the pattern of similarity of the Cryptococcus species, and discuss their useful application in epidemiological studies. We analyzed 10 isolates of each specie/group: C. albidus, C. laurentii complex, C. neoformans var. grubii, all from environmental source, and two ATCC strains, C. neoformans var. grubii ATCC 90112, and C. neoformans var. neoformans ATCC 28957 by RAPD-PCR using the primers CAV1, CAV2, ZAP19, ZAP20, OPB11 and SEQ6. The primers showed a good discriminatory power, revealing important differences between them and between species; the SEQ6 primer discriminated a larger number of isolates of three species. Isolates of C. laurentii showed greater genetic diversity than other species revealed by all six primers. Isolates of C. neoformans were more homogeneous. Only the primer CAV2 showed no amplification of DNA bands for C. albidus. It was concluded that the use of limited number of carefully selected primers allowed the discrimination of different isolates, and some primers (e.g., CAV2 for C. albidus) may not to be applied to some species.

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Biotype stability of Candida albicans isolates after culture storage determined by randomly amplified polymorphic DNA and phenotypical methods

Cited by 16 publications

References 37 publications

Comparison of two storage conditions of Candida albicans for DNA extraction and analysis

Comparison of two storage conditions of Candida albicans for DNA extraction and analysis

Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland

Comparison of primers for RAPD-PCR from environmental isolates of Cryptococcus neoformans, Cryptococcus albidus and Cryptococcus laurentii complex

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