Biotransformations with Lipases

Detergent compatible alkaline lipase produced by marine Bacillus smithii BTMS 11

“…Based on the observation that, the B. smithii lipase is stable in organic solvents methanol, propanol, ethanol, and n-hexane, it is suggested that the enzyme holds potential for application in transesterification and ester synthesis. Further it was observed that B. smithii lipase could effectively catalyze methyl-ester synthesis between fatty acids of varying carbon chain lengths and methanol and prefer medium-chain fatty acids to long chain fatty acids (C8: 0 to C18: 0) which were etherified at higher conversion rates (70 %) indicating scope for exploitation in synthesis of medium-long-medium-chain (MLM) triglycerides (Kazlauskas and Bornscheuer 1998). It must be noted that methyl and ethyl esters of long-chain fatty acids have been used to enrich diesel fuels (Vulfson 1994) and these esters have extensive uses as emulsifiers, antioxidants and flavour compounds in the food, pharmaceutical, detergent and oleochemical industries .…”

Thiol-functionalized copolymeric polyesters by lipase-catalyzed esterification and transesterification of 1,12-dodecanedioic acid and its diethyl ester, respectively, with 1-thioglycerol

“…Moreover, copolymeric polyoxoesters are commonly obtained by enzymatic polyesterification or polytransesterification of various alkanedioic acids or alkanedioates with alkanediols using immobilized lipases such as lipase B from C. antarctica (Gross et al 2001;Kazlauskas and Bornscheuer 1998), whereas , -end thiol-functionalized PE are formed by lipase-catalyzed ring-opening polymerization ofalkanelactones with various mercaptoalkanols (Takwa et al 2008). …”

“…For example, sulfur-containing polyesters may be suitable as antioxidative ingredients for polymers, coatings, paints and lubricants and as polymers with antibacterial properties (Kúdelka et al 1985;Uhrich 2003). PE having high molecular weights are formed by biotransformation of hydroxy alkanoic acids in microorganisms (Kim and Lenz 2001;Steinbüchel and Hein 2001) or by lipase-catalyzed esterification and transesterification of , -alkanedioic acids and their methyl esters, respectively, with , -alkanediols in vitro (Kazlauskas and Bornscheuer 1998;Kobayashi and Uyama 2001;Rehm 2006). In contrast to polythioesters, polyoxoesters are more easily decomposed by microbial hydrolases such as lipases or depolymerases (Jendrossek 2001;Tokiwa and Calabia 2004).…”

Comparative study of kinetic and interfacial properties of a novelRhizopus oryzaelipase and ROL29

“…Lipases are ubiquitous enzymes of considerable physiological significance and industrial potential and catalyze the hydrolysis of triacylglycerols to glycerol and free fatty acids. In contrast to esterases, lipases are activated only when adsorbed to an oil-water interface [3][4][5] and do not hydrolyse dissolved substrates in the bulk fluid. A true lipase will split emulsified esters of glycerin long-chain fatty acids such as triolein and tripalmitin.…”