Biotransformation of progesterone by Aspergillus nidulans VKPM F-1069 (wild type)

Savinova, Оlga S.; Solyev, Pavel N.; Vasina, Daria V.; Tyazhelova, T. V.; Федорова, Т.В.; Савинова, Т. С.

doi:10.1016/j.steroids.2019.05.013

Cited by 10 publications

(9 citation statements)

References 28 publications

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“…These were the species of yeast: Saccharomyces fragilis, S. lactis, Candida pseudotropicalis, Torulopsis sphaerica ( Capek et al, 1964) and fungi: Penicillum sp., Spicaria sp. (Kraychy et al, 1971), Myceliophthora thermophila (Hunter et al, 2009) and Aspergillus nidulans (Savinova et al, 2019). Although some strains belonging to the Spicaria species were able to acetylate 3b-and 17bhydroxy groups of steroids, two other strains tested by our team, S. fusispora AM136 and S. violacea AM439, catalysed the reduction of 7-oxo-DHEA (1) to 3b,17bdihydroxy-androst-5-en-7-one (2) and did not exhibit acylating activity against the substrate.…”

Section: Other Metabolitesmentioning

confidence: 99%

The catalytic activity of mycelial fungi towards 7‐oxo‐DHEA – an endogenous derivative of steroidal hormone dehydroepiandrosterone

Łyczko

Panek

Ceremuga

et al. 2021

Microbial Biotechnology

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Seventeen species of fungi belonging to thirteen genera were screened for the ability to carry out the transformation of 7-oxo-DHEA (7-oxodehydroepiandrosterone). Some strains expressed new patterns of catalytic activity towards the substrate, namely 16b-hydroxylation (Laetiporus sulphureus AM498), Baeyer-Villiger oxidation of ketone in D-ring to lactone (Fusicoccum amygdali AM258) and esterification of the 3b-hydroxy group (Spicaria divaricata AM423). The majority of examined strains were able to reduce the 17-oxo group of the substrate to form 3b,17b-dihydroxy-androst-5-en-7-one. The highest activity was reached with Armillaria mellea AM296 and Ascosphaera apis AM496 for which complete conversion of the starting material was achieved, and the resulting 17b-alcohol was the sole reaction product. Two strains of tested fungi were also capable of stereospecific reduction of the conjugated 7-keto group leading to 7b-hydroxy-DHEA (Inonotus radiatus AM70) or a mixture of 3b,7a,17btrihydroxy-androst-5-ene and 3b,7b,17b-trihydroxyandrost-5-ene (Piptoporus betulinus AM39). The structures of new metabolites were confirmed by MS and NMR analysis. They were also examined for their cholinesterase inhibitory activity in an enzymaticbased assay in vitro test.

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Section: Other Metabolitesmentioning

confidence: 99%

The catalytic activity of mycelial fungi towards 7‐oxo‐DHEA – an endogenous derivative of steroidal hormone dehydroepiandrosterone

Łyczko

Panek

Ceremuga

et al. 2021

Microbial Biotechnology

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“…Raphidocelis subcapitata , Chlorella vulgaris , Chlorococcus and Scenedesmus quadricauda belong to algae capable of removing hormones [ 11 , 26 , 40 , 41 ]. Fungi from genus Trichoderma , Trametes , Phanerochaete , Pleurotus , Aspergillus , Lentinula , Drechslera , Curvularia , Penicillium , Acremonium , Fusarium , Gibberella , Beauvaria , Doaratomyces , Scopulariopsis , Chaetomidium , Thielavia , Neurospora , Absidia , Cunninghamella , Actinomucor , Rhizopus , Phycomyces , Mortierella , Circinella and Ganoderma ( Table 2 ) were also described as being capable of hormone biodegradation/biotransformation [ 22 , 30 , 42 , 43 , 44 , 45 , 46 ].…”

Section: Microbiological Degradation Of Edcsmentioning

confidence: 99%

“…It was demonstrated that Aspergillus nidulans VKPM F-1069 hydroxylates progesterone at the position C11α to 11α-hydroxyprogesterone. Next, this compound undergoes 6β-hydroxylation or acetylation to 6β,11α-dihydroxyprogesterone and 11α-acetoxyprogesterone, respectively ( Figure 7 ) [ 45 ]. In turn, Ascomycota and Zygomycota filamentous fungi are able to effective hydroxylation at position 7α-, 7β-, 11α- and 14α of 3-oxo-4-ene androstane steroids ( Figure 8 ) [ 43 ].…”

Section: Microbiological Degradation Of Edcsmentioning

confidence: 99%

Suitability of Immobilized Systems for Microbiological Degradation of Endocrine Disrupting Compounds

Wojcieszyńska

Marchlewicz

2020

Molecules

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The rising pollution of the environment with endocrine disrupting compounds has increased interest in searching for new, effective bioremediation methods. Particular attention is paid to the search for microorganisms with high degradation potential and the possibility of their use in the degradation of endocrine disrupting compounds. Increasingly, immobilized microorganisms or enzymes are used in biodegradation systems. This review presents the main sources of endocrine disrupting compounds and identifies the risks associated with their presence in the environment. The main pathways of degradation of these compounds by microorganisms are also presented. The last part is devoted to an overview of the immobilization methods used for the purposes of enabling the use of biocatalysts in environmental bioremediation.

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“…was analysed on progesterone degradation which concluded that microbial degradation of pollutants effectively results in production of eco-friendly non-toxic end products (Yu et al, 2018). Though not much studies have been conducted addressing progesterone degradation using fungi, one previous report claimed Aspergillus niger strain VKPM F-1069 was able to tolerate the progesterone concentration in medium and successfully declined its concentration (Savinova et al, 2019). In recent days, Talaromyces sp.…”

Section: Introductionmentioning

confidence: 99%

Soil fungi, Aspergillus niger NAAC, as environmental pollution clean-up agent against Progesterone: Remediation strategy and preparation of Bioformulation

Chatterjee

Afreen

2022

Appl Env Biotechnol

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The presence of hormones, drugs and chemicals in the environment are disrupting the ecosystem. The existence of chemicals in the environment is a threat to the ecosystem as they have dangerous effects on animals, plants and microbes. Progesterones are steroid hormones used for human contraceptives and therapeutic purposes as well as promoting animal growth. The cases of consumption of progesterone for medical purposes are much higher than estrogens, however, much studies related to estrogens have been conducted thereby neglecting the effects of progesterone. This invention focuses on removal of progesterone using fungal strain. Aspergillus niger NAAC efficiently degraded the progesterone content and transformed it into non-toxic end product. The uniqueness of this study involves preparation of a formulated product which would store the fungal strain and maintain its viability. The bioformulation was prepared using used vegetable cooking oil mixed with water as carrier. The bioformulation would reduce the efforts required to isolate the microorganism for regular usage and can be commercialized for large scale applications. To determine the storage conditions of bioformulation, various parameters were analysed which showed that storing the formulation in air tight container at room temperature would result in maximum longevity of the efficient strain.

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Biotransformation of progesterone by Aspergillus nidulans VKPM F-1069 (wild type)

Cited by 10 publications

References 28 publications

The catalytic activity of mycelial fungi towards 7‐oxo‐DHEA – an endogenous derivative of steroidal hormone dehydroepiandrosterone

The catalytic activity of mycelial fungi towards 7‐oxo‐DHEA – an endogenous derivative of steroidal hormone dehydroepiandrosterone

Suitability of Immobilized Systems for Microbiological Degradation of Endocrine Disrupting Compounds

Soil fungi, Aspergillus niger NAAC, as environmental pollution clean-up agent against Progesterone: Remediation strategy and preparation of Bioformulation

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