Biotransformation of phenylurea herbicides by a soil bacterial strain, Arthrobacter sp. N2: structure, ecotoxicity and fate of diuron metabolite with soil fungi

Tixier, C.; Sancelme, Martine; Aı̈t-Aı̈ssa, Sélim; Widehem, P.; Bonnemoy, Frédérique; Cuer, A.; Truffaut, Nicole; Veschambre, H.

doi:10.1016/s0045-6535(01)00193-x

Cited by 134 publications

(129 citation statements)

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“…Several microorganisms that degrade diuron have been isolated Widehem et al, 2000), but the degradation by these microorganisms resulted in the formation of the 3,4-dichloroaniline. Further degradation of 3,4-dichloroaniline by fungi was observed by Widehem et al (2000) and Tixier et al (2002) who isolated from soil the Arthrobacter sp. N2 as a diuron-degrader.…”

Section: Introductionmentioning

confidence: 87%

Isolation of diuron-degrading bacteria from treated soil

Dellamatrice¹,

Monteiro²

2004

Braz. arch. biol. technol.

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Section: Introductionmentioning

confidence: 87%

Isolation of diuron-degrading bacteria from treated soil

Dellamatrice¹,

Monteiro²

2004

Braz. arch. biol. technol.

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“…Many AA are toxic for living organisms, and several AA are recognized as carcinogens. It has been suggested that certain soil fungi can detoxify and tolerate toxic AA (19). Therefore, fungal models are of interest to identify and understand mechanisms responsible for tolerance to toxic AA in eukaryotic organisms (7,8).…”

Section: Identification and Characterization Of P Anserina Nat Enzymmentioning

confidence: 99%

“…This compound is the major breakdown product of the phenylamide herbicides diuron, linuron, and propanil (30). The N-acetylated form of 3,4-DCA has been shown to be much less toxic than the parental compound (19). We therefore investigated whether 3,4-DCA was acetylated in vivo by WT and mutant P. anserina strains.…”

Section: Detoxification Of the Highly Toxic Pesticide Residue 34-dcamentioning

confidence: 99%

“…The potential of fungi metabolic pathways for the bioremediation of AA pollution, particularly in soils contaminated with aniline pesticide residues such as 3,4-DCA, has been little studied. Certain fungi and bacteria have nonetheless been shown to biotransform 3,4-DCA to its acetylated form (19,34). In the bacterium M. loti, a NAT isoform has been shown to acetylate 3,4-DCA (14).…”

Section: Detoxification Of the Highly Toxic Pesticide Residue 34-dcamentioning

confidence: 99%

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An Acetyltransferase Conferring Tolerance to Toxic Aromatic Amine Chemicals

Martins

Rodrigues‐Lima

Dairou

et al. 2009

Journal of Biological Chemistry

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Aromatic amines (AA) are a major class of environmental pollutants that have been shown to have genotoxic and cytotoxic potentials toward most living organisms. Fungi are able to tolerate a diverse range of chemical compounds including certain AA and have long been used as models to understand general biological processes. Deciphering the mechanisms underlying this tolerance may improve our understanding of the adaptation of organisms to stressful environments and pave the way for novel pharmaceutical and/or biotechnological applications. We have identified and characterized two arylamine N-acetyltransferase (NAT) enzymes (PaNAT1 and PaNAT2) from the model fungus Podospora anserina that acetylate a wide range of AA. Targeted gene disruption experiments revealed that PaNAT2 was required for the growth and survival of the fungus in the presence of toxic AA. Functional studies using the knock-out strains and chemically acetylated AA indicated that tolerance of P. anserina to toxic AA was due to the N-acetylation of these chemicals by PaNAT2. Moreover, we provide proof-of-concept remediation experiments where P. anserina, through its PaNAT2 enzyme, is able to detoxify the highly toxic pesticide residue 3,4-dichloroaniline in experimentally contaminated soil samples. Overall, our data show that a single xenobiotic-metabolizing enzyme can mediate tolerance to a major class of pollutants in a eukaryotic species. These findings expand the understanding of the role of xenobiotic-metabolizing enzyme and in particular of NATs in the adaptation of organisms to their chemical environment and provide a basis for new systems for the bioremediation of contaminated soils.

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“…Most studies have focused on conversion of 3,4-DCA into its acetylated form. First, it has been shown that acetylated 3,4-DCA is less toxic than 3,4-DCA; second, some soil bacteria and fungal strains acetylate 3,4-DCA 12,13 . Although aniline derivatives undergo complex transformations in soils, these studies open up new possibilities in bioremediation.…”

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confidence: 99%