Biotransformation of mogrosides from Siraitia grosvenorii by Ganoderma lucidum mycelium and the purification of mogroside III E by macroporous resins

Chiu, Chun‐Hui; Wang, Reuben; Zhuang, Shasha; Lin, Pei-Yin; Lo, Yi‐Chen; Lu, Ting‐Jang

doi:10.1016/j.jfda.2019.05.001

Cited by 15 publications

(4 citation statements)

References 32 publications

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“…The ethanol concentration exerted a significant impact on the desorption properties of D‐101 resin. Considering the cost‐effectiveness of desorption, dynamic desorption experiments with gradient ethanol aqueous solution were used to optimize the ethanol concentration (Chiu et al, 2020). The obtained fractions through different ethanol concentrations were evaluated for their lipid‐lowering activities and TTS content in this paper.…”

Section: Resultsmentioning

confidence: 99%

Response surface methodology optimization of extraction and enrichment conditions of total triterpenoid saponins from Celosiae Semen and evaluation of its lipid‐lowering activity

Yang,

Zhou,

Fan

et al. 2024

Biomedical Chromatography

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The saponin‐enriched extract from Celosiae Semen is a promising resource owing to its lipid‐lowering activity. However, triterpenoid saponins are difficult to extract owing to their high molecular weight and strong water solubility. The aim of this paper was to explore an eco‐friendly and effective technology of extraction and enrichment of total triterpenoid saponins to obtain high lipid‐lowering fractions. Initially, Box–Behnken design experiments were employed to optimize the heat reflux extraction process on the basic of mono‐factor experiments. Afterwards, the crude extract was further purified using D‐101 resin, and the purification parameters were investigated based on adsorption/desorption experiments and biological activity assay. Under optimal conditions, the purity of the finally obtained total triterpenoid saponins was increased by 7.28‐fold. The lipid‐lowering activities of the six main triterpenoid saponins were evaluated in HepG2 cells induced by palmitic acid. The results of Oil Red O staining showed that the compounds all exhibited potential lipid‐lowering activity. The structure–activity relationship analysis suggested that the oligosaccharide chain at C‐28 played an essential role in their lipid‐lowering activity and the substituent group at C‐23 site also showed important effects. The optimal extraction and purification methods may facilitate the utilization of Celosiae Semen for the industrial production as a functional food and drug.

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Section: Resultsmentioning

confidence: 99%

Response surface methodology optimization of extraction and enrichment conditions of total triterpenoid saponins from Celosiae Semen and evaluation of its lipid‐lowering activity

Yang,

Zhou,

Fan

et al. 2024

Biomedical Chromatography

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“…Third, to realize the practical applications of synthetic biology approaches, scaling-up the production of bioactive compounds and establishing economically viable processes are essential . The translation of laboratory-based research into large-scale production systems poses challenges related to cost, efficiency, and scalability . Future perspectives should focus on optimizing production systems, such as bioreactors or fermentation processes, to achieve high yields of bioactive compounds from SG. − Additionally, the development of sustainable and cost-effective downstream processing techniques is crucial for commercialization.…”

Section: Synthetic Biology Approaches For Sgmentioning

confidence: 99%

Siraitia grosvenorii As a Homologue of Food and Medicine: A Review of Biological Activity, Mechanisms of Action, Synthetic Biology, and Applications in Future Food

Guo,

Chen,

Gong

et al. 2024

J. Agric. Food Chem.

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Siraitia grosvenorii (SG), also known as Luo Han Guo or Monk fruit, boasts a significant history in food and medicine. This review delves into SG's historical role and varied applications in traditional Chinese culture, examining its phytochemical composition and the health benefits of its bioactive compounds. It further explores SG's biological activities, including antioxidant, anti-inflammatory, and antidiabetic properties and elucidates the mechanisms behind these effects. The review also highlights recent synthetic biology advances in enhancing the production of SG's bioactive compounds, presenting new opportunities for broadening their availability. Ultimately, this review emphasizes SG's value in food and medicine, showcasing its historical and cultural importance, phytochemistry, biological functions, action mechanisms, and the role of synthetic biology in its sustainable use.

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“…Mogrosides are found to be involved in activities such as scavenging of reactive oxygen species (ROS), anti-inflammatory, anticancerous, regulation of lipid metabolism of blood, and improvement of blood glucose metabolism. Among different mogrosides present in the monk fruit, mogroside III E (MG IIIE) has the most intense sweetness, and it is also found to regulate blood glucose levels efficiently; however, its relative abundance is low compared to other types of mogroside (Chiu et al 2020 ). The endophytic community associated with S. grosvenorii plant has not been much explored.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, two endophytic strains, Diaporthe angelica LHG-F5 and F. solani LHG-L4 were identified which were able to produce mogroside V (Bin et al 2020 ). A fungal ( Ganoderma lucidum )-mediated biotransformation of fruit extract to enrich MG III E by converting mogroside V to MG III E has also been reported (Chiu et al 2020 ). Therefore, endophytes associated with S. grosvenorii plants may have promising potential for the improvement of host plant health and the production of mogroside.…”

Section: Introductionmentioning

confidence: 99%

Unraveling endophytic diversity in dioecious Siraitia grosvenorii: implications for mogroside production

Tamang,

Kaur,

Thakur

et al. 2024

Appl Microbiol Biotechnol

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Host and tissue-specificity of endophytes are important attributes that limit the endophyte application on multiple crops. Therefore, understanding the endophytic composition of the targeted crop is essential, especially for the dioecious plants where the male and female plants are different. Here, efforts were made to understand the endophytic bacterial composition of the dioecious Siraitia grosvenorii plant using 16S rRNA amplicon sequencing. The present study revealed the association of distinct endophytic bacterial communities with different parts of male and female plants. Roots of male and female plants had a higher bacterial diversity than other parts of plants, and the roots of male plants had more bacterial diversity than the roots of female plants. Endophytes belonging to the phylum Proteobacteria were abundant in all parts of male and female plants except male stems and fruit pulp, where the Firmicutes were most abundant. Class Gammaproteobacteria predominated in both male and female plants, with the genus Acinetobacter as the most dominant and part of the core microbiome of the plant (present in all parts of both, male and female plants). The presence of distinct taxa specific to male and female plants was also identified. Macrococcus, Facklamia, and Propionibacterium were the distinct genera found only in fruit pulp, the edible part of S. grosvenorii. Predictive functional analysis revealed the abundance of enzymes of secondary metabolite (especially mogroside) biosynthesis in the associated endophytic community with predominance in roots. The present study revealed bacterial endophytic communities of male and female S. grosvenorii plants that can be further explored for monk fruit cultivation, mogroside production, and early-stage identification of male and female plants. Key points • Male and female Siraitia grosvenorii plants had distinct endophytic communities • The diversity of endophytic communities was specific to different parts of plants • S. grosvenorii-associated endophytes may be valuable for mogroside biosynthesis and monk fruit cultivation

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Biotransformation of mogrosides from Siraitia grosvenorii by Ganoderma lucidum mycelium and the purification of mogroside III E by macroporous resins

Cited by 15 publications

References 32 publications

Response surface methodology optimization of extraction and enrichment conditions of total triterpenoid saponins from Celosiae Semen and evaluation of its lipid‐lowering activity

Response surface methodology optimization of extraction and enrichment conditions of total triterpenoid saponins from Celosiae Semen and evaluation of its lipid‐lowering activity

Siraitia grosvenorii As a Homologue of Food and Medicine: A Review of Biological Activity, Mechanisms of Action, Synthetic Biology, and Applications in Future Food

Unraveling endophytic diversity in dioecious Siraitia grosvenorii: implications for mogroside production

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