Biotransformation of Fructose to Allose by a One-Pot Reaction Using Flavonifractor plautii D-Allulose 3-Epimerase and Clostridium thermocellum Ribose 5-Phosphate Isomerase

Lee, Tae-Eui; Shin, Kyung‐Chul; Oh, Deok-Kun

doi:10.4014/jmb.1709.09044

Cited by 20 publications

(10 citation statements)

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“…Group C3 was significantly correlated with Group C2 yet weakly negatively with Group C1. It might because Clostridium XIVa, Blautia, Parabacteroides , and Flavonifractor in Group C3 had the similar metabolic and anti-inflammatory functions with Group C1 and C2 (Kverka et al, 2011; Van den Abbeele et al, 2013; Takahashi et al, 2016; Lee et al, 2018). It also had been previously reported that Blautia (one genus in Group C3) were decreased by Lactobacillus (one genus in Group C2) (Wang R. et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Bifidobacterium and Lactobacillus were often used as probiotics to treat intestinal or systemic inflammation (Mohamadzadeh et al, 2011; Wang J. et al, 2015). Group C3 also contained some butyrate-producing bacteria, such as Clostridium XIVa and Blautia (Van den Abbeele et al, 2013; Takahashi et al, 2016), and some anti-inflammatory bacteria, like Parabacteroides and Flavonifractor (Kverka et al, 2011; Lee et al, 2018). It seemed that Group C3 had similar function with Group C1.…”

Section: Discussionmentioning

confidence: 99%

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Core Gut Bacteria Analysis of Healthy Mice

et al. 2019

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Previous studies revealed that there existed great individual variations of gut microbiota in mice, and the gut bacteria of mice were changed with the occurrence and development of diseases. To identify the core gut bacteria in healthy mice and explore their relationships with the host phenotypes would help to understand the underlying mechanisms. In this study, we identified 37 genus-level core bacteria from feces of 101 healthy mice with different ages, sexes, and mouse strains in three previous studies. They collectively represented nearly half of the total sequences, and predominantly included carbohydrate- and amino acids-metabolizing bacteria and immunomodulatory bacteria. Among them, Anaerostipes indwelt the gut of all healthy mice. Co-abundance analysis showed that these core genera were clustered into five groups (Group C1–C5), which were ecologically related. For example, the abundances of Group C2 including probiotics Bifidobacterium and Lactobacillus slightly positively correlated with those of Group C1. Principal component analysis (PCA) and multivariate analysis of variance test revealed that these core gut genera were distinguished with age and sex, and also associated with their health/disease state. Linear discriminant analysis effect size (LEfSe) method showed that bacteria in Group C1 and C2/C3 increased with the age in infancy and early adulthood, and were more abundant in female mice than in male ones. The metabolic syndrome (MS) induced by high fat diet (HFD) and accelerated postnatal growth would decrease Group C2 genera, whereas probiotics intervention would reverse HFD-induced reduction of Group C2. Spearman correlation analysis indicated that the principal components based on the abundance of the 37 core genera were significantly correlated with host characteristic parameters of MS. These results demonstrated that the 37 core genera in five co-abundance groups from healthy mice were related to host phenotypes. It was indicated that these prevalent gut bacterial genera could be representative of the healthy gut microbiome in gnotobiotic animal models, and might also be candidates of probiotics and fecal microbiota transplantation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Core Gut Bacteria Analysis of Healthy Mice

et al. 2019

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“…As no reports existed on using RpiB to prepare L-rhamnulose, the reaction conditions of other substrates were compared. For example, the conditions were pH 8.0 and temperature 70°C for using RpiB from T. maritima for L-talose isomerization [9], pH 7.5 and 65°C for using RpiB from C. thermocellum for D-allose production [8], and pH 8.0 and 75°C for using RpiB from T. lettingae for D-allose production [20]. It could be found that the reaction pH was similar, but the reaction temperature was significantly lower in the case of OsRpiB.…”

Section: 1mentioning

confidence: 99%

“…A series of RpiBs from different strains were reported to have a wide substrate spectrum and have been applied in rare sugar production. For example, D-allose could be prepared by RpiB from Thermotoga lettingae TMO, Clostridium thermocellum [8,9], Clostridium difficile, and T. maritima [10]. Furthermore, L-lyxose and L-tagatose could be prepared by RpiB from Streptococcus pneumoniae [11].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Ribose-5-Phosphate Isomerase B from Newly Isolated Strain Ochrobactrum sp. CSL1 Producing L-Rhamnulose from L-Rhamnose

Shen¹,

Ju²,

Xu³

et al. 2018

Journal of Microbiology and Biotechnology

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“…(Bhuiyan et al, 1998); by ribose-5-phosphate isomerase from Clostridium thermocellum (Park et al, 2007b) or Thermotoga lettingae (Feng et al, 2013); or by galactose 6-phosphate isomerase from Lactococcus lactis (Park et al, 2007a). Recently, a one-pot reaction method was reported to successfully produce D-allose from Dfructose using D-allulose 3-epimerase from Flavonifractor plautii and D-ribose-5-phosphate isomerase from C. thermocellum (Lee et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase

Choi

Shin

Kim

et al. 2021

Front. Bioeng. Biotechnol.

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Rare sugars are regarded as functional biological materials due to their potential applications as low-calorie sweeteners, antioxidants, nucleoside analogs, and immunosuppressants. D-Allose is a rare sugar that has attracted substantial attention in recent years, owing to its pharmaceutical activities, but it is still not widely available. To address this limitation, we continuously produced D-allose from D-allulose using a packed bed reactor with commercial glucose isomerase (Sweetzyme IT). The optimal conditions for D-allose production were determined to be pH 8.0 and 60°C, with 500 g/L D-allulose as a substrate at a dilution rate of 0.24/h. Using these optimum conditions, the commercial glucose isomerase produced an average of 150 g/L D-allose over 20 days, with a productivity of 36 g/L/h and a conversion yield of 30%. This is the first report of the successful continuous production of D-allose from D-allulose by commercial glucose isomerase using a packed bed reactor, which can potentially provide a continuous production system for industrial applications of D-allose.

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Biotransformation of Fructose to Allose by a One-Pot Reaction Using Flavonifractor plautii D-Allulose 3-Epimerase and Clostridium thermocellum Ribose 5-Phosphate Isomerase

Cited by 20 publications

References 0 publications

Core Gut Bacteria Analysis of Healthy Mice

Core Gut Bacteria Analysis of Healthy Mice

Characterization of Ribose-5-Phosphate Isomerase B from Newly Isolated Strain Ochrobactrum sp. CSL1 Producing L-Rhamnulose from L-Rhamnose

Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase

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