Biotransformation of cis-1,2-dichloroethylene to ethylene and ethane under anaerobic conditions

Komatsu, Toshiya; Momonoi, Kiyoshi; Matsuo, Tetsuji; Hanaki, Keisuke

doi:10.2166/wst.1994.0313

Cited by 13 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ethene reversibly inhibits methane formation [11]. Komatsu et al [12] found traces of ethane in anaerobic enrichment cultures originating from anaerobic sewage sludge, which were capable of dechlorinating cis-1,2-dichloroethene (cis-DCE) to ethene. In a ¢xed-bed column study, we have found that tetrachloroethene (PCE) is reductively dechlorinated to ethene under anaerobic conditions and that ethene was completely further reduced to ethane [13].…”

Section: Introductionmentioning

confidence: 99%

Anaerobic reduction of ethene to ethane in an enrichment culture

Koene-Cottaar¹

1998

FEMS Microbiology Ecology

View full text Add to dashboard Cite

A hydrogen-consuming methanogenic enrichment showed complete reduction of ethene to ethane. The ethene reduction was coupled to the formation of methane and addition of bromoethane sulfonate completely inhibited the reduction of ethene. At an ethene concentration of 0.8% in the gas phase (approximately 90 Wmol l 3I in the liquid phase), the formation of ethane from ethene was completely inhibited and methane formation was inhibited by 85%. Three dominant microorganisms were present in the enrichment. A highly motile, oval-shaped homoacetogenic bacterium, and two long, rod-shaped methanogens. One methanogen was irregularly crooked and the other one was Methanospirillum-like. The acetogen and the irregularly crooked methanogen were isolated, but in pure culture they did not reduce ethene. So far, we have been unable to isolate the other methanogen. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

show abstract

Section: Introductionmentioning

confidence: 99%

Anaerobic reduction of ethene to ethane in an enrichment culture

Koene-Cottaar¹

1998

FEMS Microbiology Ecology

View full text Add to dashboard Cite

show abstract

“…Approximately 1.93 ± 0.12 μmol of ethene was generated when 3.88 ± 0.22 μmol of TCEP was completely transformed on Day 10, meaning that 50% of TCEP was reductively transformed to produce ethene. The amount of ethane was much lower than that of ethene but stoichiometrically increased with a decline in the amount of ethene from Day 10 to Day 15, suggesting that ethene may be further converted to ethane. , Similarly, 51% of TCPP was transformed to propene, as 1.32 ± 0.26 μmol of propene was generated when 2.61 ± 0.02 μmol of TCPP was transformed on Day 15 (Figure D).…”

Section: Resultsmentioning

confidence: 99%

Dehalococcoides-Containing Enrichment Cultures Transform Two Chlorinated Organophosphate Esters

Zhu

Deng

Fang

et al. 2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

Although chlorinated organophosphate esters (Cl-OPEs) have been reported to be ubiquitously distributed in various anoxic environments, little information is available on their fate under anoxic conditions. In this study, we report two Dehalococcoides-containing enrichment cultures that transformed 3.88 ± 0.22 μmol tris(2-chloroethyl) phosphate (TCEP) and 2.61 ± 0.02 μmol tris(1-chloro-2-propyl) phosphate (TCPP) within 10 days. Based on the identification of the transformed products and deuteration experiments, we inferred that TCEP may be transformed to generate bis(2-chloroethyl) phosphate and ethene via one-electron transfer (radical mechanism), followed by C−O bond cleavage. Ethene was subsequently reduced to ethane. Similarly, TCPP was transformed to form bis(1-chloro-2-propyl) phosphate and propene. 16S rRNA gene amplicon sequencing and quantitative polymerase chain reaction analysis revealed that Dehalococcoides was the predominant contributor to the transformation of TCEP and TCPP. Two draft genomes of Dehalococcoides assembled from the metagenomes of the TCEP-and TCPP-transforming enrichment cultures contained 14 and 15 putative reductive dehalogenase (rdh) genes, respectively. Most of these rdh genes were actively transcribed, suggesting that they might contribute to the transformation of TCEP and TCPP. Taken together, this study provides insights into the role of Dehalococcoides during the transformation of representative Cl-OPEs.

show abstract

“…Conversion of cDCE and tDCE to VC and ethene also occurred but in minor amounts (van Eekert et al 2001). The conversion cDCE to ethene and ethane was observed in anaerobic sewage sludge (Komatsu et al 1994).…”

Section: Microbiology and Biochemistry Of Lower Chlorinated Ethene Bimentioning

confidence: 89%

“…Unadapted methanogenic sludges have been shown to slowly convert 1-DCE, tDCE and cDCE to VC, ethene and ethane (Komatsu et al 1994;van Eekert et al 2001), providing evidence for their reductive dehalogenation in the natural anoxic environments.…”

Section: Introductionmentioning

confidence: 99%

Biodegradability of chlorinated solvents and related chlorinated aliphatic compounds

Field

Sierra-Álvarez

2004

Rev Environ Sci Biotechnol

127

View full text Add to dashboard Cite

The biodegradability of chlorinated methanes, chlorinated ethanes, chlorinated ethenes, chlorofluorocarbons (CFCs), chlorinated acetic acids, chlorinated propanoids and chlorinated butadienes was evaluated based on literature data. Evidence for the biodegradation of compounds in all of the compound categories evaluated has been reported. A broad range of chlorinated aliphatic structures are susceptible to biodegradation under a variety of physiological and redox conditions. Microbial biodegradation of a wide variety of chlorinated aliphatic compounds was shown to occur under five physiological conditions. However, any given physiological condition could only act upon a subset of the chlorinated compounds. Firstly, chlorinated compounds are used as an electron donor and carbon source under aerobic conditions. Secondly, chlorinated compounds are cometabolized under aerobic conditions while the microorganisms are growing (or otherwise already have grown) on another primary substrate. Thirdly, chlorinated compounds are also degraded under anaerobic conditions in which they are utilized as an electron donor and carbon source. Fourthly, chlorinated compounds can serve as an electron acceptor to support respiration of anaerobic microorganisms utilizing simple electron donating substrates. Lastly chlorinated compounds are subject to anaerobic cometabolism becoming biotransformed while the microorganisms grow on other primary substrate or electron acceptor. The literature survey demonstrates that, in many cases, chlorinated compounds are completely mineralised to benign end products. Additionally, biodegradation can occur rapidly. Growth rates exceeding 1 d )1 were observed for many compounds. Most compound categories include chlorinated structures that are used to support microbial growth. Growth can be due to the use of the chlorinated compound as an electron donor or alternatively to the use of the chlorinated compound as an electron acceptor (halorespiration). Biodegradation linked to growth is important, since under such conditions, rates of degradation will increase as the microbial population (biocatalyst) increases. Combinations of redox conditions are favorable for the biodegradation of highly chlorinated structures that are recalcitrant to degradation under aerobic conditions. However, under anaerobic conditions, highly chlorinated structures are partially dehalogenated to lower chlorinated counterparts. The lower chlorinated compounds are subsequently more readily mineralized under aerobic conditions.Abbreviations: A

show abstract

Biotransformation of cis-1,2-dichloroethylene to ethylene and ethane under anaerobic conditions

Cited by 13 publications

References 0 publications

Anaerobic reduction of ethene to ethane in an enrichment culture

Anaerobic reduction of ethene to ethane in an enrichment culture

Dehalococcoides-Containing Enrichment Cultures Transform Two Chlorinated Organophosphate Esters

Biodegradability of chlorinated solvents and related chlorinated aliphatic compounds

Contact Info

Product

Resources

About