Biotransformation of bisphenol F by human and rat liver subcellular fractions

Inhibition of human carbonic anhydrase isozymes I, II and VI with a series of bisphenol, methoxy and bromophenol compounds

“…Bis(3,4-dimethoxyphenyl)methanone (6) was obtained from the reaction of 3,4-dimethoxybenzoic acid (6) with veratrole in polyphosphoric acid according to the literature method 20,22 . The Wolff−Kishner reduction of 3 with hydrazine hydrate and KOH at 190°C gave bis(3,4-dimethoxyphenyl)methane (4).…”

“…The literature procedure [14][15][16][17][18][19][20] described for o-demethylation of aryl methyl ethers with BBr 3 was applied to compounds 1 and 3 to give corresponding phenols bis(2,3-dihydroxyphenyl)methanone (3) and 4,4′-methylenedibenzen-1,2-diol (5) (Scheme 1). Compounds 2-9 were synthesised according to our previously published procedures [14][15][16][17][18][19][20][21][22] . The purification of the two CA isozymes was performed with a simple one-step method by a Sepharose-6B-aniline-sulfanilamide affinity column chromatograpy [30][31][32] .…”

“…The purification of the two CA isozymes was performed with a simple one-step method by a Sepharose-6B-aniline-sulfanilamide affinity column chromatograpy [30][31][32] . hCA I was purified, 102-fold with a specific activity of 865.7 EU/mg and overall yield of 48.4%, hCA II was purified, 625-fold with a specific activity of 5306 EU/mg and overall yield of 52.2% and hCA VI was purified, 75.3-fold with a specific activity of 213 EU/mg and overall yield of 17.8% [22][23][24][25][29][30][31][32][33][34][35] . SDS polyacrylamide gel electrophoresis was performed after the purification of these enzymes, and the electrophoretic pattern was photographed (Figure 1).…”

“…In this study, we extended these earlier investigations to bromophenols, some of which are widely used as anti-oxidant food additives or drugs. Bromophenols, frequently isolated from red algae of the family Rhodomelaceae, have some important biological activities [14][15][16][17][18][19][20][21][22] . The bisphenols or related methoxy…”

Conjugation and Deconjugation Reactions within the Fetoplacental Compartment in a Sheep Model: A Key Factor Determining Bisphenol A Fetal Exposure

“…After the addition of 3 volumes of methanol and a 10-minute centrifugation at 8000g, the supernatant was collected, and [ 3 H]-BPA-G synthesis was checked using the radio-highpressure liquid chromatography (HPLC) system described later. ]-BPA-G was purified using a 0.5 g Chromabond C18 glass cartridge (Macherey Nagel, Hoerdt, France) as described previously elsewhere (Cabaton et al, 2008 ]-BPA-S was collected and purified using a 0.5 g Chromabond C18 glass cartridge (Macherey Nagel) as described elsewhere (Cabaton et al, 2008 95%/5% atmosphere. At the end of the incubations, media and tissues were collected separately in glass vials.…”