Biotransformation of 4, 4'-dihydroxybiphenyl and dienestrol by laccase from Trametes versicolor

Lzaod, Stanzin; Dutta, Tanmay

doi:10.1016/j.hazadv.2022.100169

Cited by 4 publications

(6 citation statements)

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“…However, T. versicolor laccase has suboptimal temperature and pH working ranges as compared to LacT, which makes LacT a better candidate for biocatalytic applications in extreme conditions. Moreover, the activity of TvL is negatively affected in the presence of salt and organic solvents …”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, LacT did not have any effect on the concentration of DS (Figure a). In our previous study, we demonstrated the biotransformation of DS with TvL . There is an array of factors that can influence the catalytic activity of laccases, such as the electron donation propensity of the substrate, the redox potential of the laccase, and substrate binding to name a few .…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the activity of TvL is negatively affected in the presence of salt and organic solvents. 41 …”

Section: Resultsmentioning

confidence: 99%

“…40 The isolated strain degraded ∼98% TCS in 24 h. The bioconversion of DS and DHBP with bacterial laccase has not been outlined in the literature. Moreover, the bioconversion rate of DHBP and DS with T. versicolor laccase (TvL) is higher than that observed with LacT, as percentage removal efficiency was ∼68% for DHBP and ∼11% for DS in 1 h. 41 However, T. versicolor laccase has suboptimal temperature and pH working ranges as compared to LacT, which makes LacT a better candidate for biocatalytic applications in extreme conditions. Moreover, the activity of TvL is negatively affected in the presence of salt and organic solvents.…”

Section: Biotransformation Of Organic Contaminants Bymentioning

confidence: 99%

“…Moreover, the activity of TvL is negatively affected in the presence of salt and organic solvents. 41 On the other hand, LacT did not have any effect on the concentration of DS (Figure 7a). In our previous study, we demonstrated the biotransformation of DS with TvL.…”

Section: Biotransformation Of Organic Contaminants Bymentioning

confidence: 99%

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Thermostable Bacterial Laccase: Catalytic Properties and Its Application in Biotransformation of Emerging Pollutants

Panwar,

Lzaod,

Dutta

2023

ACS Omega

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Laccases have been predominantly reported in fungi, and primarily, fungal laccases are currently exploited in industrial applications. However, extremophilic bacterial laccases possess immense potential, as they can withstand extreme temperatures, pH, and salt concentrations. In addition, unlike fungal laccases, the production of bacterial laccases is cost-effective. Therefore, bacterial laccases are gaining significant attention for their large-scale applications. Previously, we reported a novel thermostable laccase (LacT) from Brevibacillus agri . Herein, we have confirmed that LacT shares a high sequence similarity with CotA laccase from Bacillus amyloliquefaciens . Peptide mass fingerprinting of LacT was conducted via matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF/MS-MS). Inductively coupled plasma-optical emission spectroscopic (ICP-OES) analysis revealed the presence of ∼3.95 copper ions per protein molecule. Moreover, the secondary and tertiary structure of LacT was studied using circular dichroism (CD) and fluorescence spectroscopy. The absence of notable shifts in CD and fluorescence spectra with an increase in temperature established that LacT remains intact even at elevated temperatures. Analysis of the thermal denaturation profile of LacT by thermogravimetric analysis (TGA) also confirmed its temperature stability. Thereafter, we exploited LacT in its application for the bioremediation of phenolic endocrine disruptors, namely, triclosan, 4,4′-dihydroxybiphenyl, and dienestrol. LacT oxidizes 4,4′-dihydroxybiphenyl and triclosan but no LacT activity was detected with dienestrol. The rate of biotransformation of 4,4′-dihydroxybiphenyl and triclosan increased in the presence of CuSO 4 and a redox mediator, ABTS. Transformation of dienestrol was observed only with LacT in the presence of ABTS. This study establishes the application of LacT for the bioremediation of phenolic compounds.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Moreover, the activity of TvL is negatively affected in the presence of salt and organic solvents. 41 …”

Section: Resultsmentioning

confidence: 99%

Section: Biotransformation Of Organic Contaminants Bymentioning

confidence: 99%

Section: Biotransformation Of Organic Contaminants Bymentioning

confidence: 99%