Biotransformation and liver-specific functions of human hepatocytes in culture on RGD-immobilized plasma-processed membranes

Bartolo, Loredana De; Morelli, Sabrina; Lopez, Linda C.; Giorno, Lidietta; Campana, Carla; Salerno, Simona; Rende, Maria; Favia, Pietro; Detomaso, L.; Gristina, Roberto; d’Agostino, Riccardo; Drioli, Enrico

doi:10.1016/j.biomaterials.2004.11.009

Cited by 76 publications

(45 citation statements)

References 23 publications

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“…This interaction improves cell adhesion and modulates specific metabolic responses. With an RGD amino acid sequence immobilized on polyethersulfone membranes, De Bartolo et al (2005) show the benefits in terms of the adhesion, proliferation and albumin synthesis of primary human hepatocytes.…”

Section: Alginate In Bioartificial Livermentioning

confidence: 99%

Impact of alginate type and bead diameter on mass transfers and the metabolic activities of encapsulated C3A cells in bioartificial liver applications

Gautier

Carpentier²,

Dufresne³

et al. 2011

eCM

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Liver-assist devices have been developed in the last few decades to support patients with liver failure on the road to recovery or transplantation. Fluidised bed bio-artificial livers -where liver cells are encapsulated within alginate beads -appear to be a valuable alternative to hollow fibre devices for improving mass transfers and enhancing treatment efficacy. This approach nevertheless deserves optimization in terms of bead production. The aim of this study was to investigate the impact of alginate type and of two bead diameters (1000 μm and 600 μm) on mass transfers within beads and on the biological functions of encapsulated C3A cells.After assessing the effect of the encapsulation process on bead quality, we investigated cell viability and metabolic activities (ammonia, albumin, α-foetoprotein synthesis and glucose consumption). They were successfully maintained over 48 h within fluidised bed bioreactors, independently of alginate type and bead diameter. Mass transfers were not significantly influenced by the latter parameters. Finally, suggestions are made for improving the entrapment process as a means of enhancing the treatment efficiency of the fluidised bed bioartificial liver.

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Section: Alginate In Bioartificial Livermentioning

confidence: 99%

Impact of alginate type and bead diameter on mass transfers and the metabolic activities of encapsulated C3A cells in bioartificial liver applications

Gautier

Carpentier²,

Dufresne³

et al. 2011

eCM

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“…However, RGD moieties are commonly attached to various materials by carbodiimide chemistry [26,27] and were therefore expected to remain biologically active during the gel formation. Furthermore, the MMP sensitive peptide sequence GPQG"#IWGQ was demonstrated to maintain its activity after the inclusion into hydrogel networks by EDC/sNHS chemistry.…”

Section: Resultsmentioning

confidence: 99%

Modular StarPEG‐Heparin Gels with Bifunctional Peptide Linkers

Tsurkan

Chwalek

Levental

et al. 2010

Macromol. Rapid Commun.

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Cell responsive materials are instrumental to regenerative therapies. Here, we report about a novel biohybrid hydrogel that consists of heparin and peptide-conjugated star-shaped poly(ethylene glycol), crosslinked by peptide units that combine matrix metalloproteinase (MMP) sensitivity and cell adhesive modules. Taking advantage of the high affinity of vascular endothelial growth factor to heparin, we illustrate the applicability of our hydrogels as a novel system that is supportive of cellular remodeling and three-dimensional migration of human endothelial cells.

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“…Systems based on hepatocytes of human origin are difficult to obtain because of their low viability outside the body. However, it was obtained cultures of human hepatocytes on polyethersulfone membranes having a surface modified with acrylic acid and covalently immobilized peptide (Arg-GlyAsp) [48]. One factor to consider in the case of membrane systems for the artificial liver is the design of these systems, the immobilization procedure generating unused volumes in the material structure -large open spaces not actively participating to the treatment process.…”

Section: Artificial Livermentioning

confidence: 99%