Biotoxicity of Azadirachta indica-synthesized silver nanoparticles against larvae of Culex quinquefasciatus

Aremu, Harun K.; Azeez, Luqmon; Adekale, Idris; Busari, Hassan K.; Adebayo, Zainab A.; Disu, Alimat; Usman, Halima N.; Adeyemo, Oluwatosin; Oyewole, Olu Israel

doi:10.1016/j.sajb.2023.01.021

Cited by 4 publications

(6 citation statements)

References 34 publications

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“…gambiae (s.s.) larvae subjected to methanol extract doses of 12, 25, and 125 mg/L exhibited reduced GSH concentrations compared to larvae treated with different dosages. This is consistent with the findings of Aremu et al [24], who discovered that the activity of antioxidant enzymes in Cx. quinquefasciatus mosquitoes was influenced in different ways by extracts of Azadirachta indica nanoparticles at specific concentrations.…”

Section: Plos Onesupporting

confidence: 94%

“…tenuiflorum are represented in Figs 2 and 3 respectively. The FTIR spectra revealed similar peaks at 3387–3379 cm -1 (OH stretching vibration), 2935–2754 cm -1 (C-H stretching vibrations corresponding to CH 3 and asymmetric stretching of C-H corresponding to CH 2 ), 1998–1604 cm -1 (C = C stretching modes of aromatics), 1404–1384 cm -1 (C = O of aldehyde group), 1057–1045 cm -1 (stretching of C = O), 922–817 cm -1 (OH group), 783–779 cm -1 (methylene rocking vibration; ring deformation mode) [ 24 ]. The GC-MS analysis of aqueous, and methanol crude extracts of O .…”

Section: Resultsmentioning

confidence: 99%

“…Due to the challenges associated with the use of synthetic chemicals, the use of eco-friendly and conveniently accessible plant-based alternatives has recently been proposed as a replacement [ 39 ]. Secondary metabolites found in natural products derived from plant extracts have already been shown to induce stress, alter larval development, and delay adult emergence [ 18 , 24 ]. However, there is still a lack of understanding of the toxicity mechanisms of these plant extracts, particularly at the molecular level, which has limited their potential for absolute exploration as ecofriendly plant-based alternatives.…”

Section: Discussionmentioning

confidence: 99%

“…Graphs were designed using GraphPad Prism 9.5.1 for Windows (GraphPad Software, San Diego, USA). (methylene rocking vibration; ring deformation mode) [24]. The GC-MS analysis of aqueous, and methanol crude extracts of O. tenuiflorum showed the presence of 11 and 4 bioactive compounds respectively with a percentage peak area greater than 2.0.…”

Section: Plos Onementioning

confidence: 97%

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Phytomediated stress modulates antioxidant status, induces overexpression of CYP6M2, Hsp70, α-esterase, and suppresses the ABC transporter in Anopheles gambiae (sensu stricto) exposed to Ocimum tenuiflorum extracts

Aremu,

Dare,

Adekale

et al. 2024

PLoS ONE

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The incorporation of phytoactive compounds in the management of malarial vectors holds promise for the development of innovative and efficient alternatives. Nevertheless, the molecular and physiological responses that these bioactive substances induce remain underexplored. This present study investigated the toxicity of different concentrations of aqueous and methanol extracts of Ocimum tenuiflorum against larvae of Anopheles gambiae (sensu stricto) and unraveled the possible underlying molecular pathways responsible for the observed physiological effects. FTIR and GCMS analyses of phytoactive compounds in aqueous and methanol crude extracts of O. tenuiflorum showed the presence of OH stretching vibration, C = C stretching modes of aromatics and methylene rocking vibration; ring deformation mode with high levels of trans-β-ocimene, 3,7-dimethyl-1,3,6-octatriene in aqueous extract and 4-methoxy-benzaldehyde, 1,3,5-trimethyl-cyclohexane and o-cymene in methanol extract. The percentage mortality upon exposure to methanol and aqueous extracts of O. tenuiflorum were 21.1% and 26.1% at 24 h, 27.8% and 36.1% at 48 h and 36.1% and 45% at 72 h respectively. Using reverse transcription quantitative polymerase chain reaction (RT-qPCR), down-regulation of ABC transporter, overexpression of CYP6M2, Hsp70, and α-esterase, coupled with significantly increased levels of SOD, CAT, and GSH, were observed in An. gambiae (s.s.) exposed to aqueous and methanol extracts of O. tenuiflorum as compared to the control. Findings from this study have significant implications for our understanding of how An. gambiae (s.s.) larvae detoxify phytoactive compounds.

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Section: Plos Onesupporting

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 97%

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Phytomediated stress modulates antioxidant status, induces overexpression of CYP6M2, Hsp70, α-esterase, and suppresses the ABC transporter in Anopheles gambiae (sensu stricto) exposed to Ocimum tenuiflorum extracts

Aremu,

Dare,

Adekale

et al. 2024

PLoS ONE

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“…They also reported a second SPR broadband centered around 590 nm, characteristic of nanoparticle aggregation. Recently, synthesized AgNPs with an extract of Azadirachta indica presented a surface plasmon resonance centered at 425 nm 24 …”

Section: Resultsmentioning

confidence: 99%

A crossover study of antimicrobial capacity and biotoxicity of silver nanoparticles

Sosa,

Igartúa,

Alonso

et al. 2024

Applied Organom Chemis

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Silver nanoparticles (AgNPs) have a primordial role in the nanotechnology industry since they have great antimicrobial effects against diverse pathogens. AgNP production, use, and discard have been increasing; still, there is debate about the consequences of exposure to AgNPs for animals and humans. The bioaccumulation and toxicity of AgNPs depend on several factors, including AgNP concentration, physicochemical characteristics, and aggregation. So, complete activity and toxicity profiles for each silver nanoparticle synthesized must be performed. The present work aimed to characterize the behavior of specific AgNPs, then crossover of their antimicrobial capacity against Pseudomonas aeruginosa and Staphylococcus aureus with their potential in vitro cytotoxic effects in murine fibroblasts (3T3) and human keratinocytes (HaCat) cell lines, and in vivo general and organ‐specific toxicity in zebrafish embryos and larvae. A high susceptibility of P. aeruginosa and S. aureus to low concentrations of AgNPs was observed, yet a cytotoxic effect was also detected in 3T3 and HaCat cells. Moreover, these concentrations delayed embryonic development in zebrafish embryos and presented morphological abnormalities, neurotoxicity, and cardiotoxicity in zebrafish larvae. The results highlight the importance of jointly evaluating the antimicrobial activity and the toxicological profile of each nanoparticle manufactured; this study presents a suitable platform for such crossover assessment.

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Facile synthesis and surface characterization of silver metal nanoparticles using Acorus calamus and its applications

Raguvaran,

Kalpana,

Ragavendran

et al. 2024

Inorganic Chemistry Communications

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Biotoxicity of Azadirachta indica-synthesized silver nanoparticles against larvae of Culex quinquefasciatus

Cited by 4 publications

References 34 publications

Phytomediated stress modulates antioxidant status, induces overexpression of CYP6M2, Hsp70, α-esterase, and suppresses the ABC transporter in Anopheles gambiae (sensu stricto) exposed to Ocimum tenuiflorum extracts

Phytomediated stress modulates antioxidant status, induces overexpression of CYP6M2, Hsp70, α-esterase, and suppresses the ABC transporter in Anopheles gambiae (sensu stricto) exposed to Ocimum tenuiflorum extracts

A crossover study of antimicrobial capacity and biotoxicity of silver nanoparticles

Facile synthesis and surface characterization of silver metal nanoparticles using Acorus calamus and its applications

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