Abstract:Biotinylation of deoxyguanosine at an abasic site in double-stranded oligodeoxynucleotides was studied. The biotinylation of deoxyguanosine is achieved by copper-catalyzed click reaction after the conjugation of the oligodeoxynucleotide with 2-oxohex-5-ynal. The biotinylation enables visualization of the biotinylated oligodeoxynucleotides by chemiluminescence on a nylon membrane. In order to investigate the biotinylated site, the biotinylated oligodeoxynucleotides were amplified by the DNA polymerase chain rea… Show more
A novel genotoxicity sensor was developed based on the base repair process associated with an electrochemiluminescence (ECL) detection of abasic site in a double-stranded DNA monolayer. It’s the first time...
A novel genotoxicity sensor was developed based on the base repair process associated with an electrochemiluminescence (ECL) detection of abasic site in a double-stranded DNA monolayer. It’s the first time...
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