Biotinylated thermoresponsive micelle self-assembled from double-hydrophilic block copolymer for drug delivery and tumor target

Cheng, Cheng; Wei, Hua; Shi, Baoxian; Cheng, Han; Li, Cao; Gu, Zhongwei; Cheng, Si‐Xue; Zhang, Xian‐Zheng; Zhuo, Ren‐Xi

doi:10.1016/j.biomaterials.2007.10.004

Cited by 148 publications

(99 citation statements)

References 20 publications

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“…It is also known that only a few ligands per micelle are required for cell targeting to be effective. [25,48,49] After quaternization, the reaction medium was dialyzed against water in order to eliminate unreacted biotin-bromide. Although 1 H NMR spectroscopy was not sensitive enough to detect the low amounts of biotin, the attachment of this ligand to the triblock copolymer was qualitatively confirmed by thin-layer chromatography and the characteristic pink colouring of the copolymer spot upon reaction with p-(dimethylamino) cinnamaldehyde.…”

Section: Introductionmentioning

confidence: 99%

pH‐Responsive Flower‐Type Micelles Formed by a Biotinylated Poly(2‐vinylpyridine)‐block‐poly(ethylene oxide)‐block‐poly(ε‐caprolactone) Triblock Copolymer

Butsele

Cajot

Vlierberghe

et al. 2009

Adv Funct Materials

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In the present work, a method is proposed to assemble pH‐responsive, flower‐like micelles that can expose a targeting unit at their periphery upon a decrease in pH. The micelles are composed of a novel biotinylated triblock copolymer of poly(εε‐caprolactone)‐block‐poly(ethylene oxide)‐block‐poly(2‐vinylpyridine) (PCL‐b‐PEO‐b‐P2VP) and the non‐biotinylated analogue. The block copolymers are synthesized by sequential anionic and ring‐opening polymerization. The pH‐dependent micellization behaviour in aqueous solution of the triblock copolymers developed is studied using dynamic light scattering, zeta potential, transmission electron microscopy (TEM), and fluorimetric measurements. The shielding of the biotin at neutral pH and their availability at the micelle surface upon protonation is established by TEM and surface plasmon resonance with avidin and streptavidin‐coated gold surfaces. The preliminary stealthy behavior of these pH‐responsive micelles is examined using the complement activation (CH50) test.

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Section: Introductionmentioning

confidence: 99%

pH‐Responsive Flower‐Type Micelles Formed by a Biotinylated Poly(2‐vinylpyridine)‐block‐poly(ethylene oxide)‐block‐poly(ε‐caprolactone) Triblock Copolymer

Butsele

Cajot

Vlierberghe

et al. 2009

Adv Funct Materials

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“…The micelle formation and the nature of micelles such as size were depended on the concentration as well as the constituent of the block copolymer (Yan et al 2008;Chung et al 1998). To develop thermo-sensitive carriers for the delivery of anticancer drug methotrexate (MTX), a biotin-conjugated PEG-b-P(NIPAM-co-N-hydroxymethylacrylamide) (biotin-PEG-b-(PNIPAM-co-HMAAM)) copolymer was synthesized (Cheng et al 2008). The LCST of the copolymer were adjusted by varying the molar feed ratios of NIPAM to HMAAM.…”

Section: Polymeric Micelles With Temperature-responsive Corementioning

confidence: 99%

Stimuli-Responsive Polymeric Nanocarriers as Promising Drug and Gene Delivery Systems

Saravanakumar

Kim

2014

Intracellular Delivery II

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Polymeric nanocarriers have emerged as promising drug delivery vehicles owing to their potential to selectively deliver the active agents to the disease sites through various targeting mechanisms, while minimizing the side effects. To realize more enhanced therapeutic effect, it is also highly essential to impart specific release mechanism into the nanocarriers in addition to the targeting capabilities. In this regard, stimuli-sensitive or smart polymeric nanocarriers that are responsive to inherent (e.g. pH, temperature, redox, hypoxia, enzymes, and reactive oxygen species) or external stimuli (e.g. light, ultrasound or magnetic) have received enormous attention because of their ability to control the drug release profile in a desirable fashion at the target site of action. The primary focus of this chapter is to highlight the recent advances of various stimuli-responsive nanocarriers that have been developed for a more efficient drug and gene delivery.

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“…These characteristics can grant an excellent physiological stabilities and biocompatibilities in the blood stream to achieve long-circulating time [29]. This long-circulating ability in turn makes it possible to accumulate the encapsulated drugs in the required regions via an active and/or a passive mechanism, in particular when they are used for targeting of cancer [6,7,5]. Upon our data, the poly (NIPAAm-MAA-VP) nanoparticles showed no chemical interaction with the model drug and we witnessed fairly high capacity of loading efficiencies (95-99%) for Farnesiferol C which is a hydrophobic compound.…”

Section: In Vitro Drug Releasementioning

confidence: 99%

“…Further, polymeric micelles structured from amphiphilic copolymers are regarded as one of the most promising carriers for drug delivery [5,6,7]. The self-assembled structures are composed of two basic compartments of hydrophobic core and hydrophilic corona, at which they have been used to solubilize poorly soluble drugs/compounds [8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Novel thermosensitive poly (N-isopropylacrylamide-co-vinylpyrrolidone-co-methacrylic acid) nanosystems for delivery of natural products.

Mashinchian¹,

Salehi²,

Dehghan³

et al. 2010

Int J Drug delivery

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The purpose of this research was to synthesize polymer based smart nanosystems for delivery of important bioactive natural products such as sesquiterpene coumarin derivatives of ferula szowitsiana, farnesiferol C as a potent anticancer. To this aim, polymeric micelles were prepared using Nisopropylacrylamide (NIPAAM), vinyl pyrrolidone (VP) and methacrylate (MAA) as monomers which were cross-linked with N, N-methylene bisacrylamide (MBA). The molar ratio of the PNIPAAm: VP: MAA group was 75.7:9.5:14.8. These micelles were further characterized upon their physicochemical properties using particle size analyzer, FT-IR, H-/C-NMR, HPLC. Particle size analyzer resulted in ~500 nm micelles with ~95% drug entrapments. Drug release from the polymeric micelles after 300 hours at 37°C and 40°C were 60 and 98 %, respectively. Upon these findings, it is proposed that the P (N-isopropylacrylamide-co-Methacrylic acid-coVinylpyrrolidone) micelles may be considered as thermosensitive delivery nanosystem.

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Biotinylated thermoresponsive micelle self-assembled from double-hydrophilic block copolymer for drug delivery and tumor target

Cited by 148 publications

References 20 publications

pH‐Responsive Flower‐Type Micelles Formed by a Biotinylated Poly(2‐vinylpyridine)‐block‐poly(ethylene oxide)‐block‐poly(ε‐caprolactone) Triblock Copolymer

pH‐Responsive Flower‐Type Micelles Formed by a Biotinylated Poly(2‐vinylpyridine)‐block‐poly(ethylene oxide)‐block‐poly(ε‐caprolactone) Triblock Copolymer

Stimuli-Responsive Polymeric Nanocarriers as Promising Drug and Gene Delivery Systems

Novel thermosensitive poly (N-isopropylacrylamide-co-vinylpyrrolidone-co-methacrylic acid) nanosystems for delivery of natural products.

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