Biotinylated Steroid Derivatives as Ligands for Biospecific Interaction Analysis with Monoclonal Antibodies Using Immunosensor Devices

Kaiser, Thomas; Gudat, Peter; Stock, Werner; Pappert, Gunter; Grol, Michael; Neumeier, D.; Luppa, Peter B.

doi:10.1006/abio.2000.4596

Cited by 41 publications

(28 citation statements)

References 31 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…103 Systematic ligand-binding studies of the interaction between steroids (17 testosterone and β-estradiol derivatives) and anti-steroid antibodies were performed to assess the effect of biotinylation of the steroid molecule in different positions. 104 Living endothelial cells were cultured on the piezosensor surface as the biological signal transduction element. Nocodazole, a known microtubule binding drug, alters the cytosceletal properties of living cells resulting in cell contact breaks measured as frequency changes.…”

Section: Affinity Studies Using Piezoelectric Sensorsmentioning

confidence: 99%

Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

Skládal¹

2003

J. Braz. Chem. Soc.

108

View full text Add to dashboard Cite

Esta revisão apresenta cristais piezoeletricos de quartzo como transductores aplicáveis no desenvolvimento de diferentes tipos de ensaios bioanaliticos. Os componentes do sistema de medidas para sensores tipo piezo são descritos juntamente com os sistemas de equipamentos comerciais existentes. Os biosensores piezo elétricos podem ser aplicados para determinação de vírus, bacteriais e outras células, proteínas, ácidos nucléicos e pequenas moléculas encontradas em remédios, hormônios e pesticidas. Além de mudança de massa, alguns ensaios de aglutinação empregando efeitos de viscosidade são discutidos. Finalmente, o monitoramento da afinidade das interações direto semmarcação e em tempo real usando sensores piezo é apresentado. Os pressupostos teóricos para a determinação apropriada das constantes de velocidade e de equilíbrio são apresentados e uma aproximação é demonstrada para a interação anticorpo com o correspondente antígeno (proteína secalin). Vários exemplos de estudo de afinidade são mostrados, incluindo a interação de proteínas (anticorpos e antígenos), de ácidos nucléicos (hibridização, intercalação de complexos metálicos), lipídeos e camadas com base de sacarídeos.This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.

show abstract

Section: Affinity Studies Using Piezoelectric Sensorsmentioning

confidence: 99%

Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

Skládal¹

2003

J. Braz. Chem. Soc.

108

View full text Add to dashboard Cite

show abstract

“…Quando um analito interage com a espécie imobilizada, o índice de refração da interface metal-solução sofre alteração 21,14 . O emprego da SPR como sistema de transdução em imunossensores destaca-se em investigações de modelos de interações de processos de reconhecimento molecular 22,[47][48][49][50] . Tsoi e colaboradores 22 empregaram a SPR para estudar interações entre uma série de dupla hélice de DNA (dsDNA) e DNA de enzimas polimerases.…”

Section: Aplicações Biológicas E Clínicasunclassified

SPR: Uma nova ferramenta para biossensores

2003

View full text Add to dashboard Cite

Recebido em 4/1/02; aceito em 27/6/02 SPR -NEW TOOL FOR BIOSENSORS. This paper reviewed the development and theoretical aspects of surface plasmon ressonance (SPR) technique and discusses this powerful sensor technology in the development of biosensors, as well as for the investigation of biological interactions and clinical assays. The SPR has been proven to be a valuable tool to investigate dynamic processes, such as adsorption, degradation, determination of dieletric properties, association/dissociation kinetics, affinity constants of specific ligand-ligate interactions, allowing real-time analysis at almost any surface. The SPR as a complementary technique alongside electrochemical methods is also presented.

show abstract

“…Elevated blood levels are found in about 30% of women with localized breast cancer and in about 75% of those with invasive breast cancer (cancer that has metastasized or spread to other organs) [2]. Various analytical techniques based on immunological methods have currently been exploited to assay CA 15-3, such as radioimmunoassay [3], enzyme-linked immunosorbent assay [4], flouroimmunoassay [5], chemiluminescene assay [6], surface plasmon resonance [7,8], and surface-enhanced Raman assay [9]. These methods, unfortunately, are either time-consuming, or complicated with the need for expensive instrumentation and technical skills [10,11].…”

Section: Introductionmentioning

confidence: 99%

Double‐Layer Nanogold and Double‐Strand DNA‐Modified Electrode for Electrochemical Immunoassay of Cancer Antigen 15‐3

Yang

Zhong

et al. 2008

Electroanalysis

View full text Add to dashboard Cite

Various sensor-based immunoassay methods have been extensively developed for the detection of cancer antigen 15-3 (CA 15-3), but most often exhibit low detection signals and low detection sensitivity, and are unsuitable for routine use. The aim of this work is to develop a simple and sensitive electrochemical immunoassay for CA 15-3 in human serum by using nanogold and DNA-modified immunosensors. Prussian blue (PB), as a good mediator, was initially electrodeposited on a gold electrode surface, then double-layer nanogold particles and double-strand DNA (dsDNA) with the sandwich-type architecture were constructed on the PB-modified surface in turn, and then anti-CA 15-3 antibodies were adsorbed onto the surface of nanogold particles. The double-layer nanogold particles provided a good microenvironment for the immobilization of biomolecules. The presence of dsDNA enhanced the surface coverage of protein, and improved the sensitivity of the immunosensor. The performance and factors influencing the performance of the immunosensor were evaluated. Under optimal conditions, the proposed immunosensor exhibited a wide linear range from 1.0 to 240 ng/mL with a relatively low detection limit of 0.6 ng/mL (S/N ¼ 3) towards CA 15-3. The stability, reproducibility and precision of the as-prepared immunosensor were acceptable. 57 serum specimens were assayed by the developed immunosensor and standard enzyme-linked immunosorbent assay (ELISA), respectively, and the results obtained were almost consistent. More importantly, the proposed methodology could be further developed for the immobilization of other proteins and biocompounds.

show abstract

Biotinylated Steroid Derivatives as Ligands for Biospecific Interaction Analysis with Monoclonal Antibodies Using Immunosensor Devices

Cited by 41 publications

References 31 publications

Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

SPR: Uma nova ferramenta para biossensores

Double‐Layer Nanogold and Double‐Strand DNA‐Modified Electrode for Electrochemical Immunoassay of Cancer Antigen 15‐3

Contact Info

Product

Resources

About