Biotinidase deficiency: a boy with angular cheilitis and blepharitis

Abstract

agar (Flukaâ Analytical) containing 0.05% chloramphenicol (Inlab) and Mycobiotic agar (Difco), in triplicate, and incubated at 25°C for 45 days.None of the 579 samples of nail polish (of different brands and colors) showed characteristic fungal growth for dermatophytes of the genera Trichophyton, Microsporum, and Epidermophyton and yeasts of the genus Candida sp; that is, all the cultured samples tested negative for these microorganisms. In a previous work, no fungal growth was detected on plates inoculated with nail polish contaminated with 2 9 10 3 to 6 9 10 3 UFC of T. rubrum/ml of nail polish 3 or nail polish routinely used at nail salons. 4 Although different methodologies were used, nail polish contains highly toxic chemicals such as toluene, xylene, formaldehyde, chromium, and nickel. Furthermore, the preservatives and biocides present in nail polish, including sodium benzoate, potassium sorbate, benzoic acid, and phenols, decrease contamination with microorganisms.Therefore, our findings suggest that nail polish contains chemical components that inhibit microorganism growth, so it is not a source of fungi that causes onychomycosis due to dermatophytes, yeasts of the genus Candida, and non-dermatophyte filamentous fungi.