Biotin Status and Lipid Metabolism in Adult Obese Hypercholesterolemic Inbred Rats

Abstract

A statistically significant inverse association was generally found between plasma total lipid, cholesterol, or phospholipid and biotin status of 300-day-old male inbred BHE (IN-BHE) rats. Plasma, liver, and carcass lipid of both sexes generally had a significant direct association with liver lactate dehydrogenase activity; an inverse association in males resulted with improved biotin status. Elevated plasma lactate indicative of anaerobic glycolysis was found. It is proposed that an increased reductive environment – a consequence of accumulated NADH – could account for enhanced triglyceride synthesis and that this effect could explain the obesity in the IN-BHE rats. After the injection of 300 µg of biotin, plasma levels of lactate and pyruvate fell in male rats, indicating a stimulatory effect of biotin upon the oxidative pathways in these animals.

Pharmacological effects of biotin

“…Spontaneous symptoms of biotin deficiency were detected in rats genetically prone to development of elevated blood lipids [36]. In this rat strain, an inverse association was found between plasma lipids and biotin status [37]. A negative correlation between plasma biotin concentrations and blood lipids was also found in humans [38].…”

Nutritional factors as catalyst for weight reduction

“…Tanaka et al (2010) demonstrated that thiamin intervention averted obesity and prevented metabolic disorders in Otsuka Long-Evans Tokushima Fatty (OLTF) rats but the possible mechanisms could not be explained. Marshall et al (1976) demonstrated that biotin has a stimulatory effect on oxidative pathways in 300-day-old inbred BHE (IN-BHE) rats. Jacobs et al (2010) demonstrated the mice fed with choline-deficient diets had increased oxygen consumption, had improved glucose tolerance, and gained less weight.…”

Final Report on the Safety Assessment of Biotin

“…Male inbred-BHE kine 3 rats were fed a semipuri ed diet with or without 5 mg/kg Biotin and controls were fed chow for 6 weeks (Marshall et al 1972). Feed consumption and body weights were signi cantly decreased and all relative organ-tobody weights, except relative liver weights, were increased for the animals fed the semipuri ed diet without Biotin when compared to those fed the Biotin-supplemented diet and those fed chow.…”