2001
DOI: 10.1093/jn/131.7.1909
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Biotin Regulates the Genetic Expression of Holocarboxylase Synthetase and Mitochondrial Carboxylases in Rats

Abstract: Biotin is the cofactor of carboxylases [pyruvate (PC), propionyl-CoA (PCC), 3-methyl crotonyl-CoA and acetyl-CoA], to which it is covalently bound by the action of holocarboxylase synthetase (HCS). We have studied whether biotin also regulates their expression, as it does other, nonrelated enzymes (e.g., glucokinase, phosphoenol pyruvate carboxykinase, guanylate cyclase). For this purpose, HCS, PC and PCC mRNAs were studied in biotin-deficient rat liver, kidney, muscle and brain of biotin-deficient rats. PC- a… Show more

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Cited by 72 publications
(49 citation statements)
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“…KIT or c-kit) represses miR-539 expression, thereby de-repressing microphthalmia-associated transcription factor expression and promoting proliferation (43). In other cell types, miR-539 expression may be biotin-sensitive, and miR-539 targets the mRNA of holocarboxylase synthase, which participates in genetic stability (44). Such collective findings create interesting implications for a more detailed understanding of the molecular interactions governing O-GlcNAc-dependent cell function; however, there is no evidence, at present to directly link the aforementioned observations to one another, at least at the level of O-GlcNAcylation.…”
Section: Discussionmentioning
confidence: 99%
“…KIT or c-kit) represses miR-539 expression, thereby de-repressing microphthalmia-associated transcription factor expression and promoting proliferation (43). In other cell types, miR-539 expression may be biotin-sensitive, and miR-539 targets the mRNA of holocarboxylase synthase, which participates in genetic stability (44). Such collective findings create interesting implications for a more detailed understanding of the molecular interactions governing O-GlcNAc-dependent cell function; however, there is no evidence, at present to directly link the aforementioned observations to one another, at least at the level of O-GlcNAcylation.…”
Section: Discussionmentioning
confidence: 99%
“…To confirm integrity of RNA, 22 g of RNA were electrophoresed using a formaldehyde-agarose gel (22); RNA was stained with ethidium bromide and visualized using a Kodak EDAS 290 Documentation and Analysis System (Rochester, NY). Two major ribosomal bands (28S and 18S rRNA) but no degraded RNA were detected (data not shown).…”
Section: Methodsmentioning
confidence: 99%
“…25) Furthermore, the mRNA expression of the biotin transporter sodiumdependent multivitamin transporter (SMVT) and pyruvate carboxylase was also decreased in biotin-deficient rat liver and human leukocytes. [26][27][28][29][30] In addition to these genes, the mRNA level of biotinidase was analyzed by quantitative RT-PCR. The abundance of mRNAs of HCS and SMVT remained unchanged by the biotin treatment in STZ rats (Fig.…”
Section: Effects Of Biotin On the Expression Of Transcription Factorsmentioning
confidence: 99%