Biotin determination in food supplements by an electrochemical magneto biosensor

Kergaravat, Silvina V.; Gomez, Gustavo A.; Fabiano, Silvia; Chávez, Tamara I. Laube; Pividori, María Isabel; Hernández, Silvia R.

doi:10.1016/j.talanta.2012.05.003

Cited by 40 publications

(20 citation statements)

References 54 publications

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“…The linear correlation coefficient was 0.9976. The detection limit of biotin was 6.4 nM (S/N = 3), which is comparable to those obtained by electrochemical magneto biosensor and quartz crystal microbalance sensor for biotin assays . Table illustrates a number of methods for the determination of biotin .…”

Section: Resultssupporting

confidence: 60%

“…The disadvantages of this method are poor selectivity, tedious operation, and time‐consuming. In order to overcome these disadvantages, a variety of analytical methods including HPLC , TLC , CGE , flow injection analysis , electrochemical biosensor , CZE, and ELISA have been developed for biotin detection in the last 30 years. These methods show high sensitivity, being simpler and faster than the microbiological method.…”

Section: Introductionmentioning

confidence: 99%

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A G‐quadruplex/hemin DNAzyme‐based microchip electrophoresis chemiluminescence assay for highly sensitive detection of biotin in flour

Zhao

et al. 2019

Electrophoresis

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Quantitative analysis of biotin in biological fluids, foods, and pharmaceutical is important for diagnosis and treatment of biotin‐related diseases and health maintenance. In this work, a novel G‐quadruplex/hemin DNAzyme‐based microchip electrophoresis chemiluminescence (CL) assay method was established for rapid and highly sensitive detection of biotin. This method is based on the specific binding between biotin and streptavidin, the catalytic CL characteristics of G‐quadruplex/hemin DNAzyme to the oxidation–reduction reaction of hydrogen peroxide with luminol, and the on‐line separation function of microchip electrophoresis. Under the optimal experimental conditions, on‐chip biotin analysis was achieved within 1 min. The CL intensity is linearly proportional to the concentration of biotin in the range of 13–630 nM with a detection limit of 6.4 nM. The proposed method has been applied for the detection of biotin in flour, biotin contents in three flour samples are found in the range of 199–223 ng/g with a mean value of 214 ng/g. The recoveries were in the range of 94–103%. With excellent sensitivity and good selectivity, the proposed method could be applied in a wide range of biological fluids, foods, and pharmaceutical analysis.

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Section: Resultssupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

A G‐quadruplex/hemin DNAzyme‐based microchip electrophoresis chemiluminescence assay for highly sensitive detection of biotin in flour

Zhao

et al. 2019

Electrophoresis

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“…3 times lower LoD that sensor prepared using sputtering. It should be noted that both values are similar to or better than other optical fibre devices [7,[49][50][51].…”

Section: Andsupporting

confidence: 65%

Novel Highly Sensitive Protein Sensors Based on Tapered Optical Fibres Modified with Au-Based Nanocoatings

et al. 2016

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Novel protein sensors based on tapered optical fibres modified with Au coatings deposited using two different procedures are proposed. Au-based coatings are deposited onto a nonadiabatic tapered optical fibre using (i) a novel facile method composed of layer-by-layer deposition consisting of polycation (poly(allylamine hydrochloride), PAH) and negatively charged SiO 2 nanoparticles (NPs) followed by the deposition of the charged Au NPs and (ii) the sputtering technique. The Au NPs and Au thin film surfaces are then modified with biotin in order to bind streptavidin (SV) molecules and detect them. The sensing principle is based on the sensitivity of the transmission spectrum of the device to changes in the refractive index of the coatings induced by the SV binding to the biotin. Both sensors showed high sensitivity to SV, with the lowest measured concentration levels below 2.5 nM. The calculated binding constant for the biotin-SV pair was 2.2 × 10 −11 M −1 when a tapered fibre modified with the LbL method was used, with a limit of detection (LoD) of 271 pM. The sensor formed using sputtering had a binding constant of 1.01 × 10with a LoD of 806 pM. These new structures and their simple fabrication technique could be used to develop other biosensors.

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“…The analysis is performed by a pattern recognition system. Kergaravat et al presented a biosensor based on a composite electrode where the electrochemical signal is based on the enzymatic activity of the HRP enzyme under the addition of H 2 O 2 as substrate and o‐phenilendiamine as co‐substrate.…”

Section: Water Soluble Vitaminsmentioning

confidence: 99%

“…Only two reports regarding biotin (vitamin B 7 ) are available in the last decade and both deals with the analysis of food and related samples using biosensors. Gholivand et al proposed a sensor based on a room‐temperature ionic liquid‐chitin composite film decorated with electrochemically deposited Pd‐Fe‐Ni trimetallic alloy nanoparticles.…”

Section: Water Soluble Vitaminsmentioning

confidence: 99%

Recent Advances in Electroanalysis of Vitamins

Brunetti

2016

Electroanalysis

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Vitamins are a chemically heterogeneous group of compounds known as essential for the normal functioning of the organic metabolism. Their decrease in humans can lead to several diseases and not being produced by the body, they need to be obtained from dietary sources or supplements. For these reasons, their determination in food, pharmaceutical and clinical samples is crucial. This article reviews key advances in electrochemical measurements of vitamins over the past decade. Particular attention is given to papers proving applicability to real samples analysis. The technical features of the proposed sensors are presented and discussed along with their analytical performances. Future trends and challenges are also addressed.

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Biotin determination in food supplements by an electrochemical magneto biosensor

Cited by 40 publications

References 54 publications

A G‐quadruplex/hemin DNAzyme‐based microchip electrophoresis chemiluminescence assay for highly sensitive detection of biotin in flour

A G‐quadruplex/hemin DNAzyme‐based microchip electrophoresis chemiluminescence assay for highly sensitive detection of biotin in flour

Novel Highly Sensitive Protein Sensors Based on Tapered Optical Fibres Modified with Au-Based Nanocoatings

Recent Advances in Electroanalysis of Vitamins

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