Biotic Stressors Affecting Key Apiaries in Argentina

Maggi, Matías; Quintana, Silvina; Revainera, Pablo Damián; Porrini, Leonardo Pablo; Arcerito, Facundo René Meroi; Landa, Gregorio Fernández de; Brasesco, Constanza; Gerónimo, Vanesa Di; Ruffinengo, Sergio; Eguaras, Martín Javier

doi:10.1080/0005772x.2019.1699007

Cited by 8 publications

(2 citation statements)

References 85 publications

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“…It is known that pollinators are drastically declining worldwide [ 25 , 26 ]. Different factors are contributing to this decline, like habitat loss, climate change, urbanization, agricultural intensification, pathogens spread at global level [ 27 – 29 ], all complex variables that may act in synergy [ 30 ]. Bee pathogens show lethal and sublethal effects on bees generally reducing their lifespan [ 31 ] and among pathogens, parasites have been identified as main drivers of wild bees decline [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

The gut microbiome of solitary bees is mainly affected by pathogen assemblage and partially by land use

Landa

Alberoni

Baffoni

et al. 2023

Environmental Microbiome

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Pollinators, including solitary bees, are drastically declining worldwide. Among the factors contributing to this decline, bee pathogens and different land uses are of relevance. The link between the gut microbiome composition and host health has been recently studied for social pollinators (e.g. honeybees), whereas the information related to solitary bees is sparse. This work aimed at the characterization of the gut microbiome of the solitary bees Xylocopa augusti, Eucera fervens and Lasioglossum and attempted to correlate the gut microbial composition with the presence and load of different pathogens and land uses. Solitary bees were sampled in different sites (i.e. a farm, a natural reserve, and an urban plant nursery) showing different land uses. DNA was extracted from the gut, 16S rRNA gene amplified and sequenced. Eight pathogens, known for spillover from managed bees to wild ones, were quantified with qPCR. The results showed that the core microbiome profile of the three solitary bees significantly varied in the different species. Pseudomonas was found as the major core taxa in all solitary bees analyzed, whereas Lactobacillus, Spiroplasma and Sodalis were the second most abundant taxa in X. augusti, E. fervens and Lasioglossum, respectively. The main pathogens detected with qPCR were Nosema ceranae, Nosema bombi and Crithidia bombi, although differently abundant in the different bee species and sampling sites. Most microbial taxa did not show any correlation with the land use, apart from Snodgrassella and Nocardioides, showing higher abundances on less anthropized sites. Conversely, the pathogens species and load strongly affected the gut microbial composition, with Bifidobacterium, Apibacter, Serratia, Snodgrassella and Sodalis abundance that positively or negatively correlated with the detected pathogens load. Therefore, pathogens presence and load appear to be the main factor shaping the gut microbiome of solitary bees in Argentina.

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Section: Introductionmentioning

confidence: 99%

The gut microbiome of solitary bees is mainly affected by pathogen assemblage and partially by land use

Landa

Alberoni

Baffoni

et al. 2023

Environmental Microbiome

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“…Our research group reported in previous studies that 30% of colony loses in Argentina occur in areas with temperate climates; as is the case of Buenos Aires and Santa Fé Provinces, the main honey-producing Provinces of the country (Maggi et al ., 2016). This scenario is alarming and the result of synergistic effects generated by Varroa and its ability to vector viruses in tandem with agricultural intensification (Maggi et al ., 2013, 2016). The current beekeeping situation of the country suggests the increasing necessity of more intensive mite control and care of honeybee colonies in the area.…”

Section: Introductionmentioning

confidence: 99%

Deformed wing virus type a and b in managed honeybee colonies of Argentina

Brasesco

Quintana

Gerónimo³

et al. 2020

Bull. Entomol. Res.

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Apis mellifera is infected by more than 24 virus species worldwide, mainly positive-sense, single-stranded RNA viruses of the Dicistroviridae and Iflaviridae families. Among the viruses that infect honey bees, Deformed wing virus is the most prevalent and is present as three master variants DWV-A, B, and C. Given that the ectoparasitic mite Varroa destructor vectors these virus variants, recombination events between them are expected, and variants and their recombinants can co-exist in mites and honeybees at the same time. In this study, we detect, through RT-qPCR, the presence of DWV-A and B in the same samples of adult bees from colonies of Argentina. Total RNA was extracted from pools of ten adult bees from 45 apiaries distributed across the main beekeeping Provinces of Argentina (Buenos Aires, Santa Fe, Córdoba, Santiago del Estero, Río Negro, and Mendoza); then RT-qPCR reactions were performed to detect DWV-A and B, with specific primer pairs. After the amplifications, PCR products (204 and 660 bp amplicons for DWV-B, and ~250 bp for DWV-A) were purified and sequenced to verify that they corresponded to reported sequences, analyzing them using the Blast software. Of the 45 samples analyzed by RT-qPCR, over 90% were infected with DWV-A and 47% were also positive for DWV-B, where it was found in high prevalence specifically in colonies of A. mellifera of the Buenos Aires Province. Future studies will determine the impact of this type of the virus and its ability to recombine with the other DWV types in the apiaries of our country.

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On the occurrence of the neogregarine Apicystis bombi (Apicomplexa) in South America: an unassembled puzzle

Plischuk,

Lange

2024

Biol Invasions

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Biotic Stressors Affecting Key Apiaries in Argentina

Cited by 8 publications

References 85 publications

The gut microbiome of solitary bees is mainly affected by pathogen assemblage and partially by land use

The gut microbiome of solitary bees is mainly affected by pathogen assemblage and partially by land use

Deformed wing virus type a and b in managed honeybee colonies of Argentina

On the occurrence of the neogregarine Apicystis bombi (Apicomplexa) in South America: an unassembled puzzle

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