“…The final dataset comprised 61 individuals of the A. angulatus group and five For the newly generated molecular data, we extracted genomic DNA from tissue samples stored in 100% ethanol following the protocols of the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA). We amplified the ND4 gene using primers ND4L/ND4H (Arévalo et al, 1994), following methods of Moraes et al (2020). PCR products were purified with PEG (polyethyleneglycol) 8000 and sequenced using the Big Dye Terminator sequencing kit (Applied Biosystems, Waltham, MA, USA) in the automated sequencer ABI 3130 XL (Applied Biosystems, Waltham, MA, USA) of the Laboratório Temático de Biologia Molecular (LTBM), at the Instituto Nacional de Pesquisas da Amazônia (INPA).…”