Biotechnology of the rose: a review of recent progress

Rout, Gyana Ranjan; Samantaray, Sanghamitra; Mottley, J.; Das, P.

doi:10.1016/s0304-4238(99)00025-4

Cited by 61 publications

(30 citation statements)

References 97 publications

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“…Investigations of other species of the subfamily Rosoideae, e.g. Rubus chamaemorus or Rosa sp., have mostly focused on the development of micropropagation protocols (Rout et al 1999;Thiem and Ś liwińska 2003;Martinussen et al 2004). In the present study, the accumulation of free phenolic acids and cinnamic acidthe parent compound of one subgroup of these metabolites, was investigated for the first time in the biomass from established in vitro cultures differing in the degree of tissue differentiation (shoot and callus cultures), growing on five variants of Linsmaier and Skoog (L-S) (1965) medium supplemented with various concentrations of plant growth regulators (PGRs): auxin-a-naphthaleneacetic acid (NAA), and cytokinin-N 6 -benzyladenine (BA), in the concentration range from 0.1 to 3.0 mg l -1 .…”

Section: Introductionmentioning

confidence: 99%

Accumulation of hydroxybenzoic acids and other biologically active phenolic acids in shoot and callus cultures of Aronia melanocarpa (Michx.) Elliott (black chokeberry)

Szopa

Muszyńska

2012

Plant Cell Tiss Organ Cult

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Phenolic acids are plant metabolites important in phytotherapy and also in cosmetology. In this study, proliferating shoot and callus cultures of Aronia melanocarpa were established and maintained on Linsmaier and Skoog (L-S) medium containing different levels of a-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA), ranging from 0.1 to 3.0 mg l -1 . Methanolic extracts from the biomass of these cultures and from the fruits of soil-grown plants were used to determine the amounts of free phenolic acids and cinnamic acid using the high-performance liquid chromatography (HPLC) method. Out of a total of twelve analyzed compounds, all of the extracts contained four of them: caffeic acid, p-hydroxybenzoic acid, syringic acid, and vanillic acid. Moreover, shoot extracts also contained salicylic acid (o-hydroxybenzoic acid), while callus extracts contained p-coumaric acid. On the other hand, fruit extracts also contained both salicylic acid and p-coumaric acid. The total amount of the analyzed compounds in extracts from both shoot and callus cultures depended on the L-S medium used, and varied between 103.05 and 150.95 mg 100 g -1 dry weight (DW), and between 50.23 and 81.56 mg 100 g -1 DW, respectively. Both types of culture contained higher levels of phenolic acids than the fruit extracts (32.43 mg 100 g -1 DW). In shoot cultures, p-hydroxybenzoic acid and salicylic acid were the predominant metabolites (reaching 55.14 and 78.25 mg 100 g -1 DW, respectively), while in callus cultures, p-hydroxybenzoic acid (25.60 mg 100 g -1 DW) and syringic acid (41.20 mg 100 g -1 DW) were the main compounds. In fruit extracts, salicylic acid (15.60 mg 100 g -1 DW) and p-hydroxybenzoic acid (5.29 mg 100 g -1 DW) were predominant.

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Section: Introductionmentioning

confidence: 99%

Accumulation of hydroxybenzoic acids and other biologically active phenolic acids in shoot and callus cultures of Aronia melanocarpa (Michx.) Elliott (black chokeberry)

Szopa

Muszyńska

2012

Plant Cell Tiss Organ Cult

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“…Regeneration of the rootstock Moneyway was inhibited by BAP (van der Salm et al 1996). Many other factors affecting regeneration have been discussed in a review by Rout et al (1999).…”

Section: Introductionmentioning

confidence: 99%

Dispersal and size fractionation of embryogenic callus increases the frequency of embryo maturation and conversion in hybrid tea roses

et al. 2004

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Plant regeneration from embryogenic cells of two Rosa hybrida cultivars, Kardinal and Classy, was increased by dispersing embryogenic callus in liquid medium for 3 h followed by size-fractionation to isolate proembryogenic masses that were smaller than 530 microm. Dispersed callus of three cultivars, Kardinal, Classy, and Tineke, produced 61-135 cotyledonary-stage embryos/100 mg fresh weight (FW) as compared to intact callus that had not been dispersed, which produced only zero to three cotyledonary-stage embryos/100 mg FW. Over 500 cotyledonary-stage embryos/100 mg FW callus developed from proembryogenic masses of Kardinal, Classy, and Tineke following 2 months of culture on solidified Murashige and Skoog's basal salts medium supplemented with 0.25% activated charcoal. Cotyledonary-stage embryos of Classy that developed from both dispersed callus and fractionated cells of various sizes showed a significantly higher conversion frequency to plants (28%) than cotyledonary-stage embryos isolated from intact callus (9%). The highest conversion frequencies for Kardinal (50-58%) occurred from cotyledonary-stage embryos that developed from dispersed callus and from the fraction of cells smaller than 850 microm.

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“…The environmental conditions of plant micropropagation induce morphological disorders, including hyperhydricity, curled leaves, inhibited leaf expansion, incomplete rooting and few secondary roots, anatomical disorders such as poor vascular, spongy and palisade tissues, low stomatal density, and physiological disorders such as low net photosynthetic rate and high transpiration rate. [1][2][3] These abnormal characteristics directly contribute to the low survival percentage of plantlets after transplanting to ex-vitro. However, the main cause of plantlet death after transplanting to exvitro is still unclear.…”

Section: Introductionmentioning

confidence: 99%

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Cha–um¹,

Mosaleeyanon²,

Supaibulwatana³

et al. 2003

ScienceAsia

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ABSTRACT:The objective of this study was to develop an in-vitro acclimatization technique through relative humidity reduction for direct transplanting of woody species to ex-vitro. The survival percentage, growth efficiency and physiological characteristics of in-vitro acclimatized plantlets and ex-vitro adaptation were then evaluated. Thai neem (Azadirachta siamensis Val.) was selected for this research as it is a species with high potential for regenerating soils with high salt content. Twenty-eight day-old plantlets were transferred to environmental control chambers under controlled relative humidity (RH), which are 65±5% and 95±5% for low and high RH respectively, in order to acclimatize plantlets for 42 days. It was found that leaf water content, chlorophyll content and net photosynthetic rate (NPR) of plantlets acclimatized under low RH conditions were significantly higher than those acclimatized under high RH conditions (control). The relationship of leaf water content and chlorophyll content with NPR directly affects growth efficiency, leaf area and dry weight, of plantlets. After transplanting, the water relation system [water use efficiency (WUE), stomatal conductance (G) and transpiration rate (E)] and photosynthetic system [NPR, maximum quantum yield (F v /F m ) and quantum efficiency of photosystem II (Φ PSII )] were measured at days 0, 1, 3 and 5. The plantlets acclimatized under low RH conditions showed higher physiological adaptation in G, NPR, F v /F m and Φ PSII , except E, than those acclimatized under high RH conditions. The plantlets acclimatized under low RH conditions protected water loss through the leaf tissues by the function of stomata closure as indicated by low E values. Consequently, the survival percentage of plantlets acclimatized under low RH conditions was higher by a factor of 2.3 than those acclimatized under high RH conditions. In-vitro plantlets acclimatized by reducing RH produced vigorous plantlets and directly transplanted to ex-vitro with a high survival rate of 87.5%.

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Biotechnology of the rose: a review of recent progress

Cited by 61 publications

References 97 publications

Accumulation of hydroxybenzoic acids and other biologically active phenolic acids in shoot and callus cultures of Aronia melanocarpa (Michx.) Elliott (black chokeberry)

Accumulation of hydroxybenzoic acids and other biologically active phenolic acids in shoot and callus cultures of Aronia melanocarpa (Michx.) Elliott (black chokeberry)

Dispersal and size fractionation of embryogenic callus increases the frequency of embryo maturation and conversion in hybrid tea roses

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