Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria

Adaikpoh, Barbara I.; Fernandez, Hannah N; Eustáquio, Alessandra S.

doi:10.1016/j.copbio.2022.102782

Cited by 14 publications

(20 citation statements)

References 59 publications

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“…KJ-34 has great potential for use in the development of antibiological inoculants and ecofriendly fungicides. Burkholderia bacteria are widespread, have versatile metabolism and have a range of host species, which include pathogenic and plant probiotics members (Adaikpoh et al, 2022). Burkholderia gladioli BSR3 infects rice, causing bacterial blight, and another rice seed −borne bacterium, B. glumae, causes rice grain rot (Jeong et al, 2003;Seo et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Yang

Wang²,

Wan³

et al. 2023

Front. Plant Sci.

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IntroductionPlant pathogens are one of the major constraints on worldwide food production. The antibiotic properties of microbes identified as effective in managing plant pathogens are well documented.MethodsHere, we used antagonism experiments and untargeted metabolomics to isolate the potentially antifungal molecules produced by KJ-34.ResultsKJ-34 is a potential biocontrol bacterium isolated from the rhizosphere soil of rice and can fight multiple fungal pathogens (i.e. Ustilaginoidea virens, Alternaria solani, Fusarium oxysporum, Phytophthora capsica, Corynespora cassiicola). The favoured fermentation conditions are determined and the fermentation broth treatment can significantly inhibit the infection of Magnaporthe oryzae and Botryis cinerea. The fermentation broth suppression ratio is 75% and 82%, respectively. Fermentation broth treatment disrupted the spore germination and led to malformation of hyphae. Additionally, we found that the molecular weight of antifungal products were less than 1000 Da through semipermeable membranes on solid medium assay. To search the potentially antifungal molecules that produce by KJ-34, we used comparative and bioinformatics analyses of fermentation broth before and after optimization by mass spectrometry. Untargeted metabolomics analyses are presumed to have a library of antifungal agents including benzoylstaurosporine, morellin and scopolamine.DiscussionThese results suggest that KJ-34 produced various biological control agents to suppress multiple phytopathogenic fungi and showed a strong potential in the ecological technologies of prevention and protection.

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Section: Discussionmentioning

confidence: 99%

“…Burkholderia strains produce metabolites that relate to their various functions and eukaryotic hosts (Depoorter et al, 2021;Adaikpoh et al, 2022). The best-known metabolites are bongkrekic acid (BA) and toxoflavin (TF), produced by the food-borne pathogen B. gladioli pv.…”

Section: Discussionmentioning

confidence: 99%

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Yang

Wang²,

Wan³

et al. 2023

Front. Plant Sci.

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“…6 Burkholderia bacteria proffers an alternative microbial host with unique metabolic capabilities, although its application has been slowed by limited genetic characterization and the pathogenicity of some species. 7 Four Burkholderia strains have been tested as heterologous hosts, with the soil isolate Burkholderia sp. FERM BP-3421 displaying a high perfomance.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The source of biosynthetic pathways, regulatory elements, and codon compatibility influences the success of heterologous production in a microbial host. − Hence, it is crucial to develop phylogenetically compatible production platforms Burkholderia bacteria proffers an alternative microbial host with unique metabolic capabilities, although its application has been slowed by limited genetic characterization and the pathogenicity of some species . Four Burkholderia strains have been tested as heterologous hosts, with the soil isolate Burkholderia sp.…”

Section: Introductionmentioning

confidence: 99%

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Understanding Autologous Spliceostatin Transcriptional Regulation to Derive Parts for Heterologous Expression in a Burkholderia Bacterial Host

2023

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Burkholderia β-Proteobacteria are emerging sources of natural products. We are interested in developing Burkholderia sp. FERM BP-3421 into a synthetic biology chassis to facilitate natural product discovery. FERM BP-3421 produces autologous spliceostatins on gram per liter scale. We reasoned that transcription factors and promoters that regulate spliceostatin biosynthesis would provide valuable parts for heterologous expression. Herein we demonstrate that fr9A encodes a pathway-specific transcriptional activator of spliceostatin biosynthesis. In-frame deletion of fr9A abolished spliceostatin production, which was restored by genetic complementation. Using transcriptomics and green fluorescent protein (GFP) reporter assays, we identified four fr9 promoters, three of which are activated by LuxR-type regulator Fr9A. We then constructed an Fr9A-regulated promoter system that was compared to benchmarks and effectively applied for GFP and capistruin lasso peptide expression in an optimized host background. Our findings enrich the genetic toolbox for optimizing heterologous expression and promoting the discovery and development of natural products from Burkholderia bacteria.

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