Biotechnology applied to Haematococcus pluvialis Fotow: challenges and prospects for the enhancement of astaxanthin accumulation

Le-Feuvre, Regis; Moraga-Suazo, Priscila; Gonzalez, J.M.; Martín, Sergio San; Henríquez, Vitalia; Donoso, Andrea; Agurto-Muñoz, Cristian

doi:10.1007/s10811-020-02231-z

Cited by 26 publications

(11 citation statements)

References 132 publications

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“…This was comparable to the astaxanthin content in alga H. pluvialis (about 40 mg/g DCW), which is also the highest content reported in engineered microbes so far. In addition, the astaxanthin productivity in H.…”

Section: Resultssupporting

confidence: 77%

“…In bioreactor 1, cells were cultured for 264 h with periodic feeding of the CSM-Ura, reaching a maximum astaxanthin content of 41.3 mg/g DCW (1.9 g/L) (Figures 6A and S8A). This was comparable to the astaxanthin content in alga H. pluvialis (about 40 mg/g DCW), 46 which is also the highest content reported in engineered microbes so far. In addition, the astaxanthin productivity in H. pluvialis has been estimated to be between 0.44 and 21 mg/L/d.…”

Section: Results Andmentioning

confidence: 99%

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Production of High Levels of 3S,3′S-Astaxanthin in Yarrowia lipolytica via Iterative Metabolic Engineering

Zhu

Jiang

et al. 2022

J. Agric. Food Chem.

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Astaxanthin is a highly value-added keto-carotenoid compound. The astaxanthin 3S,3′S-isomer is more desirable for food additives, cosmetics, and pharmaceuticals due to health concerns about chemically synthesized counterparts with a mixture of three isomers. Biosynthesis of 3S,3′S-astaxanthin suffers from limited content and productivity. We engineered Yarrowia lipolytica to produce high levels of 3S,3′S-astaxanthin. We first assessed various β-carotene ketolases (CrtW) and β-carotene hydroxylases (CrtZ) from two algae and a plant. HpCrtW and HpCrtZ from Haematococcus pluvialis exhibited the strongest activity in converting β-carotene into astaxanthin in Y. lipolytica. We then fine-tuned the HpCrtW and HpCrtZ transcriptional expression by increasing the rounds of gene integration into the genome and applied a modular enzyme assembly of HpCrtW and HpCrtZ simultaneously. Next, we rescued leucine biosynthesis in the engineered Y. lipolytica, leading to a five-fold increase in biomass. The astaxanthin production achieved from these strategies was 3.3 g/L or 41.3 mg/g dry cell weight under fed-batch conditions, which is the highest level reported in microbial chassis to date. This study provides the potential for industrial production of 3S,3′S-astaxanthin, and this strategy empowers us to build a sustainable biorefinery platform for generating other value-added carotenoids in the future.

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Section: Resultssupporting

confidence: 77%

Section: Results Andmentioning

confidence: 99%

Production of High Levels of 3S,3′S-Astaxanthin in Yarrowia lipolytica via Iterative Metabolic Engineering

Zhu

Jiang

et al. 2022

J. Agric. Food Chem.

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“…Achieving a high vegetative cell density in a short period of time before being subjected to the stress conditions is a time-saving and cost-effective way to attain high astaxanthin production (Khoo et al, 2019;Nagappan et al, 2019). Although progress has been made in vegetative H. pluvialis cultivation, rapidly achieving high biomass during massive cultivation in vegetative stage remains a challenge and most of the photobioreactor (PBR) cultures have been facing the obstacle of low yield/cost ratio (Colusse et al, 2021;Le-Feuvre et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Optimizing the growth of Haematococcus pluvialis based on a novel microbubble-driven photobioreactor

Ying

Zhou

et al. 2021

iScience

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Haematococcus pluvialis, the richest bioresource for natural astaxanthin, encounters a challenge of achieving high growth rate when it comes to mass biomass production. Based on the substrate consumption model and Redfield ratio, rapid algae growth benefits from a proper carbon supply. However, the conventional cultivation schemes with limited carbon dioxide (CO 2 ) supply and inefficient carbon mass transfer could have constrained the carbon capture and growing ability of H. pluvialis. We hypothesize that optimal H. pluvialis growth improvement may be achieved by efficient CO 2 supply. Here, in this study, we first identified the carbon consumption of H. pluvialis during exponential growth. Then, a novel microbubble-driven photobioreactor (MDPBR) was designed to satisfy the carbon demand. The novel microbubble photobioreactor improves the CO 2 supply by reducing bubble size, significantly elevating the CO 2 mass transfer. With only 0.05 L min À1 of gas flow rate, higher cell growth rate (0.49 d À1 ) has been achieved in MDPBR.

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“…Although H. pluvialis is the best natural source of astaxanthin known to date, its productivity still needs to be improved. Furthermore, the ability to produce additional high-value compounds in addition to astaxanthin will enhance economic value of H. pluvialis (Le-Feuvre et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress

Song

Huang

et al. 2021

Front. Plant Sci.

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To elucidate the mechanism underlying increased fatty acid and astaxanthin accumulation in Haematococcus pluvialis, transcriptome analysis was performed to gain insights into the multiple defensive systems elicited by salicylic acid combined with sodium acetate (SAHS) stresses with a time course. Totally, 112,886 unigenes and 61,323 non-repeat genes were identified, and genes involved in carbon metabolism, primary and secondary metabolism, and immune system responses were identified. The results revealed that SA and NaAC provide both energy and precursors to improve cell growth of H. pluvialis and enhance carbon assimilation, astaxanthin, and fatty acids production in this microalga with an effective mechanism. Interestingly, SA was considered to play an important role in lowering transcriptional activity of the fatty acid and astaxanthin biosynthesis genes through self-protection metabolism in H. pluvialis, leading to its adaption to HS stress and finally avoiding massive cell death. Moreover, positive correlations between 15 key genes involved in astaxanthin and fatty acid biosynthesis pathways were found, revealing cooperative relation between these pathways at the transcription level. These results not only enriched our knowledge of the astaxanthin accumulation mechanism in H. pluvialis but also provided a new view on increasing astaxanthin production in H. pluvialis by a moderate and sustainable way in the future.

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Biotechnology applied to Haematococcus pluvialis Fotow: challenges and prospects for the enhancement of astaxanthin accumulation

Cited by 26 publications

References 132 publications

Production of High Levels of 3S,3′S-Astaxanthin in Yarrowia lipolytica via Iterative Metabolic Engineering

Production of High Levels of 3S,3′S-Astaxanthin in Yarrowia lipolytica via Iterative Metabolic Engineering

Optimizing the growth of Haematococcus pluvialis based on a novel microbubble-driven photobioreactor

Haematococcus pluvialis Accumulated Lipid and Astaxanthin in a Moderate and Sustainable Way by the Self-Protection Mechanism of Salicylic Acid Under Sodium Acetate Stress

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