Biotechnology Applications of Grapevine Viruses

Dolja, Valerian V.; Meng, Bin

doi:10.1007/978-3-319-57706-7_31

Cited by 1 publication

(1 citation statement)

References 60 publications

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“…Like most perennial plants, grapevines are affected by a large number of pathogens, including viruses that belong to different taxonomic groups, due to their accumulation during long-term cultivation [1][2][3]. Most often, grapevine viruses are spread by vectors, vegetative propagation through plant cuttings and germplasm [4]. Therefore, the use of virus-free germplasm from the early breeding stages is of particular importance.…”

Section: Introductionmentioning

confidence: 99%

Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia)

Shvets

Porotikova

Sandomirsky

et al. 2022

Viruses

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Grapevine germplasm collections are unique repositories of grape cultivars; therefore, it is necessary to minimize their infection with pathogens, including viruses, and develop various programs to maintain them in a virus-free state. In our study, we examined the virome of the largest Russian grapevine germplasm collection, the Anapa Ampelographic Collection, using high-throughput sequencing of total RNAs. As a result of bioinformatics analysis and validation of its results by reverse transcription PCR (RT-PCR) and quantitative RT-PCR (RT-qPCR), we identified 20 viruses and 3 viroids in 47 libraries. All samples were infected with 2 to 12 viruses and viroids, including those that cause economically significant diseases: leafroll, fleck, and rugose wood complex. For the first time in Russia, we detected Grapevine virus B (GVB), Grapevine virus F (GVF), Grapevine asteroid mosaic-associated virus (GAMaV), Grapevine Red Globe virus (GRGV), Grapevine satellite virus (GV-Sat), Grapevine virga-like virus (GVLV), Grapevine-associated jivivirus 1 (GaJV-1) and Vitis cryptic virus (VCV). A new putative representative of the genus Umbravirus with the provisional name Grapevine umbra-like virus (GULV) was also identified in Russian grape samples.

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