Biotechnological prospecting of foliar endophytic fungi of guaco (Mikania glomerata Spreng.) with antibacterial and antagonistic activity against phytopathogens

Polônio, Julio Cesar; Almeida, Tiago Tognolli de; García, Adriana; Mariucci, Giovanna Emanuêlle Gonçalves; Azevedo, João Lúcio de; Rhoden, Sandro Augusto; Pamphile, João Alencar

doi:10.4238/2015.july.3.5

Cited by 26 publications

(23 citation statements)

References 36 publications

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“…In Brazil, biotechnological research has targeted the diversity of microorganisms because of the potential for discovery of new bioactive compounds or bioprocesses (Schulz and Boyle, 2005;de Oliveira Costa et al, 2012;García et al, 2012b;Rhoden et al, 2012;Miguel et al, 2013;Polonio et al, 2015). Studies on microbiota associated with plants are important to understand and predict the spatial distribution of microbial communities and the ecosystem responses to global climate change (Singh et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Rhizosphere bacteriome of the medicinal plant Sapindus saponaria L. revealed by pyrosequencing

et al. 2016

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ABSTRACT. Sapindus saponaria L. of Sapindaceae family is popularly known as soldier soap and is found in Central and South America. A study of such medicinal plants might reveal a more complex diversity of microorganisms as compared to non-medicinal plants, considering their metabolic potential and the chemical communication between their natural microbiota. Rhizosphere is a highly diverse microbial habitat with respect to both the diversity of species and the size of the community. Rhizosphere bacteriome associated with medicinal plant S. saponaria is still poorly known. The objective of this study was to assess the rhizosphere microbiome of the medicinal plant S. saponaria using pyrosequencing, a cultureindependent approach that is increasingly being used to estimate the number of bacterial species present in different environments. In their rhizosphere microbiome, 26 phyla were identified from 5089 sequences of 16S rRNA gene, with a predominance of Actinobacteria (33.54%), Acidobacteria (22.62%), and Proteobacteria (24.72%). The rarefaction curve showed a linear increase, with 2660 operational taxonomic units at 3% distance sequence dissimilarity, indicating that the rhizosphere microbiome associated with S. saponaria was highly diverse with groups of bacteria important for soil management, which could be further exploited for agricultural and biotechnological purposes.

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Section: Introductionmentioning

confidence: 99%

Rhizosphere bacteriome of the medicinal plant Sapindus saponaria L. revealed by pyrosequencing

et al. 2016

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“…CNPUV378 (provided by EMBRAPA Grape and Wine, Bento Gonçalves, RS, Brazil). For this, the dual culture method of Campanile, Ruscelli, and Luisi (2007) modified by Polonio et al (2015) was used. All assay was performed in triplicate.…”

Section: In Vitro Antagonism and Competitive Interactions Between Endmentioning

confidence: 99%

“…The percentage inhibition rate of mycelial growth (IP%) was calculated following Polonio et al (2015): IP% = (1 -MT MC -1 ) x 100, where IP% = inhibition index percentage of mycelial growth, MT = mean of triplicate area measured for treatment in cm 2 , and MC = average of triplicate control area in cm 2 .…”

Section: In Vitro Antagonism and Competitive Interactions Between Endmentioning

confidence: 99%

“…There were 3 mycelia plugs (6mm diameter) of seven-day-old cultures of each endophyte inoculated into 500-mL Erlenmeyer flasks containing 250 mL of PDB (Potato Dextrose Broth; pH 6.8) (Himedia, Mumbai, India) and incubated at 28ºC for 21 days under stationary condition. The metabolic extraction from the fermented culture broth, by using ethyl acetate P.A (Synth, Diadema, SP, Brazil) as solvent, followed the protocol described by Polonio et al (2015).…”

Section: Screening Of Antifungal Activity Of Metabolic Extractsmentioning

confidence: 99%

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Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit

Oliveira

Polli

Polônio

et al. 2020

Acta Sci. Biol. Sci.

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Endophytic microorganisms live inside the plants without causing any damage to their hosts. In the agricultural field, these endophytes might be a strategy of biological control for phytopathogens. We aimed to isolate endophytic fungi from yellow passion fruit (Passiflora edulis) leaves, evaluating its biocontrol capacity by in vitro antagonism against phytopathogen Colletotrichum sp. CNPU378. We also carried out greenhouse experiments in bean seedlings. A high colonization frequency was obtained (89%), and the molecular identification based on DNA sequencing attested Colletotrichum as the most frequent genus and minor occurrence of Curvularia endophytes. The endophytes tested showed different types of competitive interactions in in vitro antagonism inhibition rate ranging from 28.8 to 48.8%. There were 10 promising antagonists tested for their antagonist activity of crude extracts of secondary metabolites, in which strain PE-36 (20.8%) stood out among the other strains evaluated. In the greenhouse assay, plants inoculated only with endophyte Colletotrichum sp. PE-36 was symptomless and suggest that the endophyte strengthened the growth promotion in common bean plants, especially in the root length and number of leaves when compared to control plants and other treatments. Despite many fungi of Colletotrichum genus being described as causative agents of anthracnose, in this study, the plant sampled was colonized predominantly by Colletotrichum endophytes living in asymptomatic relationship. By the way, we come across a Colletotrichum sp. endophyte able to antagonize a Colletotrichum sp. pathogen.

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“…In fact, the bulk of FEF research is represented by studies focusing on a specific host plant (Jumpponen & Jones, 2010;Zimmerman & Vitousek, 2012;Mejía et al, 2014;Saucedo-García et al, 2014;Oono et al, 2015;Polonio et al, 2015;Felber et al, 2016;González-Teuber, 2016;Kato et al, 2017). Studies that have surveyed FEF or other leaf-associated fungi across multiple plant species have found significant host effects (Unterseher et al, 2012;Kembel & Mueller, 2014;Massimo et al, 2015;Huang et al, 2016;Vincent et al, 2016), suggesting that host identity likely interacts with the abiotic environment to structure FEF community composition.…”

Section: Introductionmentioning

confidence: 99%

Fungal communities living within leaves of native Hawaiian dicots are structured by landscape-scale variables as well as by host plants

Darcy

Cobian

Swift

et al. 2019

Preprint

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AIM: A phylogenetically diverse array of fungi live within healthy leaf tissue of dicotyledonous plants. Many studies have examined these endophytes within a single plant species and/or at small spatial scales, but landscape-scale variables that determine their community composition are not well understood, either across geographic space, across climatic conditions, or in the context of host plant phylogeny. Here, we evaluate the contributions of these variables to endophyte community composition using our survey of foliar endophytic fungi in native Hawaiian dicots sampled across the Hawaiian archipelago. LOCATION: Hawaiʻi.METHODS: The Hawaiian archipelago offers a uniquely tractable system to study biogeography of foliar endophytic fungi, because the islands harbor a wide array of climatic conditions, and native plant species are often found across wide elevational and climactic ranges. We used Illumina technology to sequence fungal ITS1 amplicons in order to characterize foliar endophyte communities in the leaves of 896 plants across 5 islands and 80 host plant genera. Using Generalized Dissimilarity Modeling (GDM) we tested the effect of landscape-scale variables on observed differences in foliar endophyte communities. Bipartite network analysis was used to examine the extent to which each island harbored specialized or cosmopolitan foliar endophytes.RESULTS: Communities of foliar endophytic fungi in the Hawaiian archipelago are structured most strongly by evapotranspiration, elevation, vegetation/habitat type, and by the phylogeny of host plants. The five islands we sampled each harbored significantly specialized endophyte communities as well.MAIN CONCLUSIONS: Factors that structure foliar endophyte communities at small geographic and narrow host phylogenetic scales are broadly generalizable to the larger scales we studied here, although not universally. Evapotranspiration, a variable with resolution 250 m 2 , was the most robust predictor of endophyte community dissimilarity in our study, although it had not previously been considered an important determinant of FEF communities.

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Biotechnological prospecting of foliar endophytic fungi of guaco (Mikania glomerata Spreng.) with antibacterial and antagonistic activity against phytopathogens

Cited by 26 publications

References 36 publications

Rhizosphere bacteriome of the medicinal plant Sapindus saponaria L. revealed by pyrosequencing

Rhizosphere bacteriome of the medicinal plant Sapindus saponaria L. revealed by pyrosequencing

Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit

Fungal communities living within leaves of native Hawaiian dicots are structured by landscape-scale variables as well as by host plants

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