Biotechnological production of poly(3-hydroxybutyrate) with<i>Wautersia eutropha</i>by application of green grass juice and silage juice as additional complex substrates

Koller, Martin; Bona, Rodolfo; Hermann, Carmen; Horvat, Predrag; Martinz, Julia; Neto, José Pereira de Queiroz; Pereira, Luis; Varila, Paula; Braunegg, Gerhart

doi:10.1080/10242420500292252

Cited by 73 publications

(47 citation statements)

References 22 publications

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“…8,9 Already existing processes, like the microbial PHA-production, can become more efficient due to lower feedstock costs. 10 Numerous investigations have been made into alfalfa press juices. These studies demonstrate that alfalfa press juices can act as a substitute for MRS, a synthetic nutrient, in lactic acid production, cutting processing costs.…”

Section: Introductionmentioning

confidence: 99%

Cultivation and Fractionation of Leguminous Biomass for Lactic Acid Production

Papendiek¹

2014

Chem.Biochem.Eng.Q.

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Section: Introductionmentioning

confidence: 99%

Cultivation and Fractionation of Leguminous Biomass for Lactic Acid Production

Papendiek¹

2014

Chem.Biochem.Eng.Q.

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“…1.3 (7) × 100 (8) (4) denotes annual production of particular metabolite in 100 m 3 bioreactor, (5) denotes bioreactor scale, (6) denotes final concentration of the metabolite (depending on the cultivation mode), (7) denotes factor for the fermentation broth ∼ total volume of bioreactor, (8) denotes Factor for the calculation of hundreds of kg [18].…”

Section: Evaluation Of Fermentation Strategiesmentioning

confidence: 99%

“…The cycle time (t c ) was calculated by the addition of the laboratory scale fermentation time plus 12 h for the preparation and follow-up treatment of the bioreactor. The following equations were used for all calculations [18]. (2) t c [h] (3) where (1) denotes production cycles per year, (2) denotes production hours per year, (3) denotes duration of production cycle plus 12 h (typical value for the preparation and follow-up treatment of the bioreactor),…”

Section: Evaluation Of Fermentation Strategiesmentioning

confidence: 99%

Bioconversion of spent coffee grounds into carotenoids and other valuable metabolites by selected red yeast strains

Petrik

Obruča

Benešová

et al. 2014

Biochemical Engineering Journal

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“…This strategy was successfully demonstrated by using green grass juice, which was anaerobically fermented towards ensilage; pressed silage juice, rich in lactic acid, minerals, and nitrogen-containing compounds, turned out to be a feasible, stable feedstock to thrive the PHA producing organism Cupriavidus necator (43 processing equipment) into such already running industrial production lines, where the feedstock is directly generated as a waste stream. For ethical reasons, any conflict with competing applications of the feedstock, especially use for human nutrition, has to be circumvented in order to secure food safety (6).…”

Section: Available Raw Materials For Pha Biosynthesismentioning

confidence: 99%

Advanced approaches to produce polyhydroxyalkanoate (PHA) biopolyesters in a sustainable and economic fashion

Koller

Braunegg

2018

The EuroBiotech Journal

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Polyhydroxyalkanoates (PHA), the only group of "bioplastics" sensu stricto, are accumulated by various prokaryotes as intracellular "carbonosomes". When exposed to exogenous stress or starvation, presence of these microbial polyoxoesters of hydroxyalkanoates assists microbes to survive."Bioplastics" such as PHA must be competitive with petrochemically manufactured plastics both in terms of material quality and manufacturing economics. Cost-effectiveness calculations clearly show that PHA production costs, in addition to bioreactor equipment and downstream technology, are mainly due to raw material costs. The reason for this is PHA production on an industrial scale currently relying on expensive, nutritionally relevant "1 st -generation feedstocks", such as like glucose, starch or edible oils. As a way out, carbon-rich industrial waste streams ("2 nd -generation feedstocks") can be used that are not in competition with the supply of food; this strategy not only reduces PHA production costs, but can also make a significant contribution to safeguarding food supplies in various disadvantaged parts of the world. This approach increases the economics of PHA production, improves the sustainability of the entire lifecycle of these materials, and makes them unassailable from an ethical perspective.In this context, our EU-funded projects ANIMPOL and WHEYPOL, carried out by collaborative consortia of academic and industrial partners, successfully developed PHA production processes, which resort to waste streams amply available in Europe. As real 2 nd -generation feedstocks", waste lipids and crude glycerol from animal-processing and biodiesel industry, and surplus whey from dairy and cheese making industry were used in these processes. Cost estimations made by our project partners determine PHA production prices below 3 € (WHEYPOL) and even less than 2 € (ANIMPOL), respectively, per kg; these values already reach the benchmark of economic feasibility.The presented studies clearly show that the use of selected high-carbon waste streams of (agro)industrial origin contributes significantly to the cost-effectiveness and sustainability of PHA biopolyester production on an industrial scale.

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Biotechnological production of poly(3-hydroxybutyrate) withWautersia eutrophaby application of green grass juice and silage juice as additional complex substrates

Cited by 73 publications

References 22 publications

Cultivation and Fractionation of Leguminous Biomass for Lactic Acid Production

Cultivation and Fractionation of Leguminous Biomass for Lactic Acid Production

Bioconversion of spent coffee grounds into carotenoids and other valuable metabolites by selected red yeast strains

Advanced approaches to produce polyhydroxyalkanoate (PHA) biopolyesters in a sustainable and economic fashion

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