Biotechnological production of bio-based long-chain dicarboxylic acids with oleogenious yeasts

Abstract

Long-chain α,ω-dicarboxylic acids (DCAs) are versatile chemical intermediates of industrial importance used as building blocks for the production of polymers, lubricants, or adhesives. The majority of industrial long-chain DCAs is produced from petro-chemical resources. An alternative is their biotechnological production from renewable materials like plant oil fatty acids by microbial fermentation using oleogenious yeasts. Oleogenious yeasts are natural long-chain DCA producers, which have to be genetically engineered for high-yield DCA production. Although, some commercialized fermentation processes using engineered yeasts are reported, bio-based long-chain DCAs are still far from being a mass product. Further progress in bioprocess engineering and rational strain design is necessary to advance their further commercialization. The present article reviews the basic strategies, as well as novel approaches in the strain design of oleogenious yeasts, such as the combination of traditional metabolic engineering with system biology strategies for high-yield long-chain DCA production. Therefore a detailed overview of the involved metabolic processes for the biochemical long-chain DCA synthesis is given.

Effect of decanoic acid and 10-hydroxydecanoic acid on the biotransformation of methyl decanoate to sebacic acid

“…Additionally, the accumulation of 10-hydroxydecanoic acid was always observed after a long time in biotransformation, usually after more than 60 h of the cultivation time (more than 40 h of biotransformation) even without increasing the methyl decanoate feeding rate (data not shown). Previously, evidence for a similar bottleneck in the production of dodecanedioic acid by Yarrowia lipolytica has been provided (Gatter et al 2014 ; Werner and Zibek 2017 ). Further investigations are necessary to confirm these phenomena.…”

“…The entire conversion process is referred to as the α,ω-oxidation pathway (Kester and Foster 1963 ; Ratledge 1984 ). The mechanisms of oxidation in this pathway have been extensively studied (Eschenfeldt et al 2003 ; Cheng et al 2005 ; Huf et al 2011 ; Werner and Zibek 2017 ). Remarkably, this biotransformation process appears to be more feasible today for producing DCAs, and metabolic engineering of E. coli could be a breakthrough technology for production of these compounds either from glucose or fatty acids in the future.…”

Cellular and metabolic engineering of oleaginous yeastYarrowia lipolyticafor bioconversion of hydrophobic substrates into high‐value products

“…Although the Candida tropicalis , as one of the oleaginous yeast, has been proved to demonstrate the most significantly production of the DCA (130–140 g/L) in the means of the combining metabolic and fermentation engineering , the characteristic of the pathogenicity contributing to several hydrolytic enzyme secretion and in the favor of the non‐renewable fossil oil‐derived alkanes as carbon source are two main concerns of this strain . Hence, development of engineered Y. lipolytica has gained more attention due to the low pathogenic grade (BSL1) and the easy assimilation of renewable plant oil to LCDA .…”

Conformationally adaptable macrocyclic receptors for ditopic anions: analysis of chelate cooperativity in aqueous containing media

“…Furthermore, polyanionic species exist preferentially in an aqueous environment, which adds further complications in the design of chemical entities for the selective discrimination of these species in their native environment. Because of their biological [3][4][5] and industrial 1,6,7 importance, the development of sensors which can selectively bind and detect polyanionic species is a signicant challenge and has commanded special attention from the chemical community.…”