Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae

Zhang, Lijie; Liu, Qiuyuan; Ge, Yubin; Li, Lixiang; Gao, Chao; Xu, Ping; Ma, Cuiqing

doi:10.1039/c5gc01638j

Cited by 48 publications

(35 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, as shown in Table 3, the acetoin yields (g g −1 dry biomass) from pretreated and un-pretreated lignocellulosic biomass through different methods were still at relatively low levels. Among them, the highest acetoin production to date (45.60 g L −1 ) was achieved by metabolically engineered Enterobacter cloacae in fed-batch fermentation of lignocellulosic hydrolysate of the pretreated corn stover at 37 °C [23]. Besides, high concentration of acetoin (19.00 g L −1 ) was also achieved by Escherichia coli mlc-XT7-LAFC-YSD utilizing saccharified cedar solution in batch SHF at 37 °C [24].…”

Section: Discussionmentioning

confidence: 99%

“…(g L −1 )Pro. b (g L −1 h −1 )Refs. B. subtilis IPE5-4-UD-4Pretreated corncobBatch SSF, 5-L bioreactor50; 5022.760.38This study K. pneumoniae CICC 10011Pretreated Jerusalem artichoke stalksBatch SSF, 5-L bioreactor37; 3713.470.20[9] E. coli CelluloseBatch SSF, shake flask37; 372.700.02[13] E. cloacae SDM 53Pretreated corn stoverFed-batch SHF, 7.5-L bioreactorNA; 3745.601.52[23] K. pneumoniae CICC 10011Pretreated Jerusalem artichoke stalk and tuberFed-batch SHF, 5-L bioreactor50–55; 3711.400.16[9] E. coli DSM02-BPretreated brown seaweedFed-batch SHF, shake flask50; 374.800.07[12] E. coli mlc-XT7-LAFC-YSDPretreated Japanese cedar wood chipsBatch SHF, shake flask37; 3019.000.16[24] K. pneumoniae CICC 10011Pretreated Jerusalem artichoke stalk and tuberBatch SHF, 5-L bioreactor50–55; 3711.800.23[9]Engineered Zymomonas mobilis 22C–BC5Pretreated corn stoverBatch SHF, shake flask50; 33> 10.00NA[10] Tem...…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Conversion of cellulose and hemicellulose of biomass simultaneously to acetoin by thermophilic simultaneous saccharification and fermentation

Jia

Peng

Liu

et al. 2017

Biotechnol Biofuels

View full text Add to dashboard Cite

BackgroundAcetoin (3-hydroxy-2-butanone), the precursor of biofuel 2,3-butanediol, is an important bio-based platform chemical with wide applications. Fermenting the low-cost and renewable plant biomass is undoubtedly a promising strategy for acetoin production. Isothermal simultaneous saccharification and fermentation (SSF) is regarded as an efficient method for bioconversion of lignocellulosic biomass, in which the temperature optima fitting for both lignocellulose-degrading enzymes and microbial strains.ResultsA thermotolerant (up to 52 °C) acetoin producer Bacillus subtilis IPE5-4 which simultaneously consumed glucose and xylose was isolated and identified. By compound mutagenesis, the mutant IPE5-4-UD-4 with higher acetoin productivity was selected. When fermenting at 50 °C in a 5-L bioreactor using glucose as the feedstock by strain IPE5-4-UD-4, the acetoin concentration reached 28.83 ± 0.91 g L−1 with the acetoin yield and productivity of 0.34 g g−1 glucose and 0.60 g L−1 h−1, respectively. Furthermore, an optimized and thermophilic SSF process operating at 50 °C was conducted for acetoin production from alkali-pretreated corncob (APC). An acetoin concentration of 12.55 ± 0.28 g L−1 was achieved by strain IPE5-4-UD-4 in shake flask SSF, with the acetoin yield and productivity of 0.25 g g−1 APC and 0.17 g L−1 h−1. Meanwhile, the utilization of cellulose and hemicellulose in the SSF approach reached 96.34 and 93.29%, respectively. When further fermented at 50 °C in a 5-L bioreactor, the concentration of acetoin reached the maximum of 22.76 ± 1.16 g L−1, with the acetoin yield and productivity reaching, respectively, 0.46 g g−1 APC and 0.38 g L−1 h−1. This was by far the highest acetoin yield in SSF from lignocellulosic biomass.ConclusionsThis thermophilic SSF process provided an efficient and economical route for acetoin production from lignocellulosic biomass at ideal temperature for both enzymatic hydrolysis and microbial fermentation.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0924-8) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Conversion of cellulose and hemicellulose of biomass simultaneously to acetoin by thermophilic simultaneous saccharification and fermentation

Jia

Peng

Liu

et al. 2017

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…for synthesis of novel optically active -hydroxyketone derivatives, pharmaceutical precursors and liquid crystal composites (Xiao and Lu, 2014). (3R)-acetoin, the enantiomer of (3S)-acetoin, can be formed readily from pyruvate by enzymatic reactions involving α-acetolactate synthase (ALS) and α-acetolactate decarboxylase (ALDB) and efficient production of this isomer has been reported previously, either using naturally occurring or engineered microorganisms (Wang et al, 2015;Zhang et al, 2014;Zhang et al, 2016). (3S)-acetoin is difficult to prepare using purely enzymatic transformations, but can be formed from diacetyl or meso-2,3-butanediol as reported by Ui et al, 1984 andGao et al, 2013, who used non-growing cells as biocatalyst.…”

Section: Acc E P Ted P R E P R I Ntmentioning

confidence: 99%

Integrating biocompatible chemistry and manipulating cofactor partitioning in metabolically engineered Lactococcus lactis for fermentative production of (3S)‐acetoin

Liu

Solem

Jensen

2016

Biotech & Bioengineering

View full text Add to dashboard Cite

Biocompatible chemistry (BC), that is, non‐enzymatic chemical reactions compatible with living organisms, is increasingly used in conjunction with metabolically engineered microorganisms for producing compounds that do not usually occur naturally. Here we report production of one such compound, (3S)‐acetoin, a valuable precursor for chiral synthesis, using a metabolically engineered Lactococcus lactis strain growing under respiratory conditions with ferric iron serving as a BC component. The strain used has all competing product pathways inactivated, and an appropriate cofactor balance is achieved by fine‐tuning the respiratory capacity indirectly via the hemin concentration. We achieve high‐level (3S)‐acetoin production with a final titer of 66 mM (5.8 g/L) and a high yield (71% of the theoretical maximum). To the best of our knowledge, this is the first report describing production of (3S)‐acetoin from sugar by microbial fermentation, and the results obtained confirm the potential that lies with BC for producing useful chemicals. Biotechnol. Bioeng. 2016;113: 2744–2748. © 2016 Wiley Periodicals, Inc.

show abstract

“…It can also be used as a fuel additive due to its combustion heat energy of 27.2 kJ/g Yang et al 2015). Furthermore, 2,3-BD can be readily dehydrogenated into acetoin (AC) and diacetyl (DA) compounds, which are used as flavor enhancers in dairy products (Guo et al 2016;Zhang et al 2016). Some microbial fermentation processes have been developed to enhance the 2,3-BD production in recent years.…”

Section: Introductionmentioning

confidence: 99%

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Yuan¹,

He²,

Guo³

et al. 2017

BioResources

View full text Add to dashboard Cite

Sweet sorghum juice (SSJ) is considered a good carbon source for biorefinery due to its low price and high fermentable-sugar content. In this study, 2,3-butanediol (2,3-BD) production from SSJ by Serratia marcescens H30 was investigated. First, the medium compositions including the contents of SSJ, nitrogen source, and mineral salts were optimized in conical flasks using a single factor and orthogonal design method. Under the optimal conditions, the 2,3-BD concentration reached up to 33.40 g/L. Then the optimized medium was used to perform fermentative experiments in a 5-L bioreactor. In batch experiments, the effects of various agitation speeds on 2,3-BD production were compared. Based on batch process results, an efficient two-stage fermentative control strategy was developed, where the agitation speed was maintained at 300 rpm in the first 12 h and subsequently switched to 200 rpm. About 43.32 g/L 2,3-BD was obtained by using this strategy. Finally, fed-batch fermentation was conducted through feeding the concentrated SSJ and a maximum 2,3-BD concentration of 109.44 g/L with the productivity of 1.40 g/L·h; a yield of 83.02% was achieved. The results showed that SSJ could be used as an economical substrate for efficient 2,3-BD production by S. marcescens H30.

show abstract

Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae

Abstract: Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae.

Cited by 48 publications

References 46 publications

Conversion of cellulose and hemicellulose of biomass simultaneously to acetoin by thermophilic simultaneous saccharification and fermentation

Conversion of cellulose and hemicellulose of biomass simultaneously to acetoin by thermophilic simultaneous saccharification and fermentation

Integrating biocompatible chemistry and manipulating cofactor partitioning in metabolically engineered Lactococcus lactis for fermentative production of (3S)‐acetoin

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Contact Info

Product

Resources

About