Biotechnological Potential of Bacillus salmalaya 139SI: A Novel Strain for Remediating Water Polluted with Crude Oil Waste

Abstract

Environmental contamination by petroleum hydrocarbons, mainly crude oil waste from refineries, is becoming prevalent worldwide. This study investigates the bioremediation of water contaminated with crude oil waste. Bacillus salamalaya 139SI, a bacterium isolated from a private farm soil in the Kuala Selangor in Malaysia, was found to be a potential degrader of crude oil waste. When a microbial population of 108 CFU ml-1 was used, the 139SI strain degraded 79% and 88% of the total petroleum hydrocarbons after 42 days of incubation in mineral salt media containing 2% and 1% of crude oil waste, respectively, under optimum conditions. In the uninoculated medium containing 1% crude oil waste, 6% was degraded. Relative to the control, the degradation was significantly greater when a bacteria count of 99 × 108 CFU ml-1 was added to the treatments polluted with 1% oil. Thus, this isolated strain is useful for enhancing the biotreatment of oil in wastewater.

Optimization and Statistical Evaluation of Medium Components Affecting Crude Oil Biodegradation by Some Locally Isolated Bacteria

“…This was agreed with previous studies, which have shown that degradation of oil increased with increasing pH, and that optimum degradation occurred under slightly alkaline conditions (Venosa and Zhu, 2003). The optical density for Bacillus salmalaya 139SI remediating water polluted with crude oil waste, was the highest, at a pH 7, followed by pH 8, pH 6 and pH 5 (Ismail and Dadrasnia, 2015). This finding is supported by the idea that pH affects the activity of the enzyme, if too extreme then the enzymes will be denatured.…”

“…All the isolates in the present study were mesophilic in nature; they all exhibited optimum activity at 35ºC (Banat, 1995;Shahriari et al, 2014 andKavynifard et al, 2015). The highest and lowest growth values for Bacillus salmalaya 139SI remediating water polluted with crude oil waste were recorded at 40°C and 25°C, respectively (Ismail and Dadrasnia, 2015). Some studies showed that a temperature drop from 25 to 5ºC caused a tenfold decrease in response.…”

Biosurfactant Production by Bacillus salmalaya for Lubricating Oil Solubilization and Biodegradation

“…Many studies have reported the ability of microbial surfactants to degrade hydrocarbons. In our previous study, we reported the potential of B. salmalaya 139SI for use in the bioremediation of crude oil waste [3,12]. Ayed et al , [2] reported that the biodegradation of diesel oil was enhanced by the addition of B. amyloliquefaciens An6 compared with an anionic surfactant and Tween 80.…”

“…Selected 16S rRNA universal primers, namely, 27Forward (5′-AGAGTTTGATCMTGGCTCAG-3′) and 1492Reverse (5′- GGTTACCTTGTTACGACTT -3′), were used to amplify the 16S rRNA region [12]. …”

Effects of Bacillus salmalaya Strain 139SI Inoculation on Yield and Nutrients Uptake of Oil Palm

“…The strain 139SI originally isolated from rhizosphere soil obtained from the agricultural farm. The species classification of this strain was based on phenotypic characteristics, phylogenetic analysis and 16S rRNA G+C characterization (Gen Bank accession No: JF825470.1; ATCC BAA-2268) (Ismail and Dadrasnia, 2015). The strain 139SI was maintained in BBL TM Brain Heart Infusion (BHI) slants at 4°C until required.…”