Biotechnological potential of a novel tannase-acyl hydrolase from Aspergillus sydowii using waste coir residue: Aqueous two-phase system and chromatographic techniques

Albuquerque, Kátia K.S. A.; Albuquerque, Wendell; Costa, Romero Marcos Pedrosa Brandão; Batista, Juanize Matias da Silva; Marques, Daniela de Araújo Viana; Bezerra, Raquel Pedrosa; Herculano, Polyanna Nunes; Porto, Tatiana Souza

doi:10.1016/j.bcab.2019.101453

Cited by 20 publications

(13 citation statements)

References 36 publications

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“…In the present study, Bacillus nitratireducens showed 2.9 U/mL tannase activity at 35 °C, pH 7.0, 48 h. The SDS-PAGE analysis of the produced tannase showed a 97 kDa band and is presented in Figure S2 in the Supporting Information. Similar bands of tannase at ∼97 kDa, 90 kDa, , 87.3 kDa, and 50 kDa were reported by various researchers.…”

Section: Results and Discussionsupporting

confidence: 89%

Studies on the Effect of Bacterial Tannase Supplementation to Biodegradation of Tannins in Tannery Wastewater

Balakrishnan

Kanchinadham

Chitra

2021

Ind. Eng. Chem. Res.

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Tannins present in the vegetable tanning process wastewater (VTW) affect the aerobic microbes and reduce the treatment efficiency in the tannery effluent treatment plants. Studies were performed with the supplementation of crude tannase enzyme to improve the efficiency of aerobic treatment of composite wastewater from raw to vegetable tanning process (CWRVT). Screening, isolation, optimization of growth conditions, and sequencing of the bacterial isolate Bacillus nitratireducens were studied. Aerobic reactors were operated in continuous mode for a period of 210 days, with and without tannase supplementation. The reactors with and without tannase supplementation showed 84%− 88% and 44%−47% tannin removal, respectively, for a maximum organic loading rate (OLR) of 30.5 g COD/L d with a tannin concentration of 1000 mg/L. Confirmation of tannin degradation was done with the help of electrospray ionization−mass spectrometry (ESI-MS) and pyrogallol analysis. The kinetic parameters were derived based on Contois model considering tannin as a substrate. For the treatment of 1 m 3 of CWRVT, the supplementation of tannase enzyme will add an additional cost of Rs.30.58/-toward the cost of chemicals in the overall operation and maintenance cost of the treatment process. This study suggests that supplementation of tannase enzyme in existing treatment plants will enhance the efficiency of tannin removal.

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Section: Results and Discussionsupporting

confidence: 89%

Studies on the Effect of Bacterial Tannase Supplementation to Biodegradation of Tannins in Tannery Wastewater

Balakrishnan

Kanchinadham

Chitra

2021

Ind. Eng. Chem. Res.

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“…Cellulase was produced under submerged fermentation [70] and xylanases under solid-state fermentation [71]. Moreover, tannases [72] and lignocellulosic enzymes offer possibilities to food and bioenergy applications [73]. We can add keratinases the list of A. sydowiii for the first time.…”

Section: Discussionmentioning

confidence: 99%

Keratinases Produced by Aspergillus stelliformis, Aspergillus sydowii, and Fusarium brachygibbosum Isolated from Human Hair: Yield and Activity

Alwakeel

Ameen

Gwaiz

et al. 2021

JoF

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Twenty fungal strains belonging to 17 species and isolated from male scalp hair were tested for their capacity to hydrolyze keratinous material from chicken feather. The identification of the three most efficient species was confirmed by sequencing of the internal transcribed spacer (ITS) region of rDNA. Activities of fungal keratinases produced by Aspergillus stelliformis (strain AUMC 10920), A. sydowii (AUMC 10935), and Fusarium brachygibbosum (AUMC 10937) were 113, 120, and 130 IU mg−1 enzymes, respectively. The most favorable conditions were at pH 8.0 and 50 °C. Keratinase activity was markedly inhibited by EDTA and metal ions Ca+2, Co+2, Ni+2, Cu+2, Fe+2, Mg+2, and Zn+2, with differences between the fungal species. To the best of our knowledge, this is the first study on the activity of keratinase produced by A. stelliformis, A. sydowii, and F. brachygibbosum. F. brachygibbosum keratinase was the most active, but the species is not recommended because of its known phytopathogenicty. Aspergillus sydowii has many known biotechnological solutions and here we add another application of the species, as producer of keratinases. We introduce A. stelliformis as new producer of active fungal keratinases for biotechnological solutions, such as in the management of keratinous waste in poultry industry.

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“…Tannase production from solid-state fermentation using coir waste as a substrate was performed. In the reactors, 10 g of the substrate were added and subsequently incubated at 30 • C for 48 h [76]. The tannase-biotransformed grape pomace extracts were evaluated to prove intestinal anti-inflammatory activity using an in vitro colon model.…”

Section: Agro-industrial Wastes To Obtain Tannasesmentioning

confidence: 99%

Application of Lactic Acid Bacteria in Fermentation Processes to Obtain Tannases Using Agro-Industrial Wastes

et al. 2021

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Bacteria have been used in the food industry to produce flavors, dyes, thickeners, and to increase food value, because bacterial fermentations favor the obtention of different metabolites such as tannins and different nutritional compounds in food. Lactiplantibacillus plantarum was one the first species to be studied for industrial purposes, and its efficacy to obtaining tannins using fermentation processes. Bacterial fermentation helps to obtain a product with an added value of better quality and without the need to use strong solvents that can reduce their quality and safety. To release tannins, it is necessary to subject the substrate to different conditions to activate the enzyme tannin acyl hydrolase (tannase). The tannase-released compounds can have beneficial effects on health such as antioxidant, anticancer and cardioprotective properties, among others. Therefore, this review analyzes tannase release and other metabolites by fermentation processes.

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Biotechnological potential of a novel tannase-acyl hydrolase from Aspergillus sydowii using waste coir residue: Aqueous two-phase system and chromatographic techniques

Cited by 20 publications

References 36 publications

Studies on the Effect of Bacterial Tannase Supplementation to Biodegradation of Tannins in Tannery Wastewater

Studies on the Effect of Bacterial Tannase Supplementation to Biodegradation of Tannins in Tannery Wastewater

Keratinases Produced by Aspergillus stelliformis, Aspergillus sydowii, and Fusarium brachygibbosum Isolated from Human Hair: Yield and Activity

Application of Lactic Acid Bacteria in Fermentation Processes to Obtain Tannases Using Agro-Industrial Wastes

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