Biotechnological manipulation of the transition from genetic to temperature-dependent sex determination to obtain high quality neomale in aquaculture

Xiong, Yang; Han, Qingqing; Liu, Ying; Wang, Shuai; Yang, Jinhu; Jiang, Wei; Hu, Jingqi; Chen, Jian; Li, Pei; Mei, Jie

doi:10.1016/j.aquaculture.2022.738471

Cited by 5 publications

(3 citation statements)

References 57 publications

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“…This morphological difference between sexes prompted us to reveal mechanisms that control sex determination during embryonic development. Due to the fact that sex determination is usually controlled by a combination of genetic and environmental influences and is highly plastic [ 57 ], we were able to create YY super-male and XX neo-male yellow catfish through sex reversal technology and genetic technology [ 28 , 30 , 31 ]. The Y and X sperm generated from these male fish and the offspring of hybrid and X oocytes were excellent materials with which to detect sexual differences from early embryonic development.…”

Section: Discussionmentioning

confidence: 99%

“…So, YY super-males are sources to obtain Y sperm, while XX neo-male fish are considered as good sources to isolate X sperm. In this research, XX neo-male yellow catfish were obtained through artificial sex reversal according to our previous studies [ 30 , 31 , 32 ]. They were also two years old (139.02 ± 11.72 g) and sexually mature when selected as experimental fish.…”

Section: Methodsmentioning

confidence: 99%

“…Sex determination is a highly plastic process in lower vertebrates including reptiles, amphibians and fishes [ 12 , 15 , 27 ], which provides us with chances to perform sex-controlling breeding and reveal the mechanisms of sexual dimorphism from the development of germ cells to embryos. In past years, the YY super-male and XX neo-male yellow catfish were successfully created by sex reversal technology and identified by the robust sex-specific molecular markers [ 28 , 29 , 30 , 31 ], which is an excellent experimental model to explore the Y and X spermatozoa. In this study, we compared gene expression and genomic DNA methylation between X and Y spermatozoa (from XX neo-male and YY super-male, respectively) in yellow catfish.…”

Section: Introductionmentioning

confidence: 99%

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Sexually Dimorphic Gene Expression in X and Y Sperms Instructs Sexual Dimorphism of Embryonic Genome Activation in Yellow Catfish (Pelteobagrus fulvidraco)

Xiong

Wang

Guo

et al. 2022

Biology

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Paternal factors play an important role in embryonic morphogenesis and contribute to sexual dimorphism in development. To assess the effect of paternal DNA on sexual dimorphism of embryonic genome activation, we compared X and Y sperm and different sexes of embryos before sex determination. Through transcriptome sequencing (RNA-seq) and whole-genome bisulfite sequencing (WGBS) of X and Y sperm, we found a big proportion of upregulated genes in Y sperm, supported by the observation that genome-wide DNA methylation level is slightly lower than in X sperm. Cytokine–cytokine receptor interaction, TGF-beta, and toll-like receptor pathways play important roles in spermatogenesis. Through whole-genome re-sequencing (WGRS) of parental fish and RNA-seq of five early embryonic stages, we found the low-blastocyst time point is a key to maternal transcriptome degradation and zygotic genome activation. Generally, sexual differences emerged from the bud stage. Moreover, through integrated analysis of paternal SNPs and gene expression, we evaluated the influence of paternal inheritance on sexual dimorphism of genome activation. Besides, we screened out gata6 and ddx5 as potential instructors for early sex determination and gonad development in yellow catfish. This work is meaningful for revealing the molecular mechanisms of sex determination and sexual dimorphism of fish species.

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