Biotechnological, biomedical, and agronomical applications of plant protease inhibitors with high stability: A systematic review

Cotabarren, Juliana; Lufrano, Daniela; Parisi, Mónica Graciela; Obregón, Walter David

doi:10.1016/j.plantsci.2019.110398

Cited by 51 publications

(56 citation statements)

References 202 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Further, PI from plant sources cause a restricted digestion process and nutritional deficiencies over susceptible insects (Zhu-Salzman and Zeng, 2015;Singh et al, 2018), affecting proper insect development, fecundity and survival of different pests (Ruan et al, 2015;Vasudev and Sohal, 2016). Therefore, future experiments should explore the individual effect of the soybean meal enriched in PI over the mortality, development, weight, and fertility of H. hampei (Cotabarren et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Soybean protease inhibitors increase Bacillus thuringiensis subs. israelensis toxicity against Hypothenemus hampei

et al. 2020

View full text Add to dashboard Cite

Introduction. The coffee berry borer (Hypothenemus hampei Ferrari, CBB) is one of the most devastating pests on coffee plantations around the world. Although CBB is susceptible to the effect of δ–endotoxins of Bacillus thuringiensis subs. israelensis (Bti) at laboratory level, the efficacy of this control method is poor in the field, presumably due to the inactivation by digestive proteases different to those required for protoxin activation. Objective. To study whether the addition of a soybean flour extract enriched with protease inhibitors (PI), mixed with Bti crystals and spores (Bti-sc) in an artificial diet, could improve the toxicity of Bti against CBB. Materials and methods. This study was performed in San José, Costa Rica, between 2012 and 2013. A set of adult female CBB insects was exposed to a mixture containing different concentrations of a partially purified soybean meal extract with active PI and lyophilized Bti-sc, and were tested through a bioassay in artificial diet to estimate the sub-lethal concentration (LC50). The mortality results were validated by observing the dissected midgut, whose ultrastructure was analyzed by transmission electron microscopy. Results. The soybean extracts partially degraded the Bti-sc complex, it reduced its LC50 by almost four times (from 1.135 to 0.315 µg µl-1) and enhanced CBB mortality in a concentration-dependent manner. Histological analyses of the midgut confirmed this synergistic effect, since severe epithelial damage to the intestinal epithelium of CBB exposed to Bti-sc + PI was visualized compared to Bti-sc alone. Conclusions. The combination of a soybean extract enriched in PI and Bti-sc enhanced the mortality effect over CBB, which was confirmed by the midgut collapse. Soybean flour is a cost-effective supplement that could increase Bti effectiveness against CBB and delay the appearance of biological resistance.

show abstract

Section: Discussionmentioning

confidence: 99%

Soybean protease inhibitors increase Bacillus thuringiensis subs. israelensis toxicity against Hypothenemus hampei

et al. 2020

View full text Add to dashboard Cite

show abstract

“…According to our previous studies, protease inhibitors present high physicochemical stability with minimal loss of inhibitory activity [26]. Accordingly, in the first purification step, the crude extract was subjected to 100 • C for 15, 60, and 120 min.…”

Section: Identification and Purification Of Mopi 231 Partial Purification By Heat Treatmentmentioning

confidence: 99%

“…A more detailed dose-response analysis showed that MoCE strongly inhibits papain with an IC 50 value of 0.025 µg/mL. A number of plant protease inhibitors (PIs) have been purified and found to be stable and active at high temperatures [26,31]. For this reason, we incubated the MoCE at 100 • C for 15, 60 and 120 min as an initial purification step.…”

Section: Isolation and Purification Of Mopimentioning

confidence: 99%

Purification and Characterization of a Novel Thermostable Papain Inhibitor from Moringa oleifera with Antimicrobial and Anticoagulant Properties

et al. 2021

Self Cite

View full text Add to dashboard Cite

Plant cystatins (or phytocystatins) comprise a large superfamily of natural bioactive small proteins that typically act as protein inhibitors of papain-like cysteine proteases. In this report, we present the purification and characterization of the first phytocystatin isolated from Moringa oleifera (MoPI). MoPI has a molecular mass of 19 kDa and showed an extraordinary physicochemical stability against acidic pHs and high temperatures. Our findings also revealed that MoPI is one of the most potent cysteine protease inhibitors reported to date, with Ki and IC50 values of 2.1 nM and 5.7 nM, respectively. More interestingly, MoPI presents a strong antimicrobial activity against human pathogens such as Enterococcus faecalis and Staphylococcus aureus. In addition, MoPI also showed important anticoagulant activity, which is an unprecedented property for this family of protease inhibitors. These results highlight the pharmaceutical potential of this plant and its derived bioactive molecules.

show abstract

“…Ингибиторы -важный компонент иммунологической системы растений (Вилкова и Конарев, 2010; Конарев, 2017). В связи с этим на протяжении уже нескольких десятилетий большие надежды возлагаются на ингибиторы в связи с потенциальной возможностью использовать их в сельском хозяйстве как средство борьбы с вредными для растений микроорганизмами и насекомыми (Ryan, 1990;Dunaevsky et al, 2005;Yarullina et al, 2016;Akbar et al, 2018;Singh et al, 2018;Cotabarren et al, 2020;). У живых организмов охарактеризовано около 100 типов белковых ингибиторов протеаз, отличающихся по структуре и механизмам действия (Rawlings et al, 2004(Rawlings et al, , 2017.…”

Section: ингибиторы протеазunclassified

Digestive Hydrolases of Wheat Bugs: Properties, Significance and Possible Ways to Limit Their Activity

Konarev¹

2020

PPN

View full text Add to dashboard Cite

Biotechnological, biomedical, and agronomical applications of plant protease inhibitors with high stability: A systematic review

Cited by 51 publications

References 202 publications

Soybean protease inhibitors increase Bacillus thuringiensis subs. israelensis toxicity against Hypothenemus hampei

Soybean protease inhibitors increase Bacillus thuringiensis subs. israelensis toxicity against Hypothenemus hampei

Purification and Characterization of a Novel Thermostable Papain Inhibitor from Moringa oleifera with Antimicrobial and Anticoagulant Properties

Digestive Hydrolases of Wheat Bugs: Properties, Significance and Possible Ways to Limit Their Activity

Contact Info

Product

Resources

About