Biotechnological approaches to determine the impact of viruses in the energy crop plant Jatropha curcas

Ramkat, Rose C.; Calari, Alberto; Maghuly, Fatemeh; Laimer, Margit

doi:10.1186/1743-422x-8-386

Cited by 23 publications

(12 citation statements)

References 47 publications

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“…Hence, a comprehensive study on the characterization of each viral strain in different crop plants is needed for the protection and improvement of crops. If neglected, begomoviruses could have a huge negative impact on field plantation and crop productivity (Ramkat et al, 2011b).…”

Section: Introductionmentioning

confidence: 99%

Isolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L.

Chauhan

Rahman

Mastan

et al. 2018

Gene

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Begomoviruses belong to the family Geminiviridae are associated with several disease symptoms, such as mosaic and leaf curling in Jatropha curcas. The molecular characterization of these viral strains will help in developing management strategies to control the disease. In this study, J. curcas that was infected with begomovirus and showed acute leaf curling symptoms were identified. DNA-A segment from pathogenic viral strain was isolated and sequenced. The sequenced genome was assembled and characterized in detail. The full-length DNA-A sequence was covered by primer walking. The genome sequence showed the general organization of DNA-A from begomovirus by the distribution of ORFs in both viral and anti-viral strands. The genome size ranged from 2844 bp-2852 bp. Three strains with minor nucleotide variations were identified, and a phylogenetic analysis was performed by comparing the DNA-A segments from other reported begomovirus isolates. The maximum sequence similarity was observed with Euphorbia yellow mosaic virus (FN435995). In the phylogenetic tree, no clustering was observed with previously reported begomovirus strains isolated from J. curcas host. The strains isolated in this study belong to new begomoviral strain that elicits symptoms of leaf curling in J. curcas. The results indicate that the probable origin of the strains is from Jatropha mosaic virus infecting J. gassypifolia. The strains isolated in this study are referred as Jatropha curcas leaf curl India virus (JCLCIV) based on the major symptoms exhibited by host J. curcas.

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Section: Introductionmentioning

confidence: 99%

Isolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L.

Chauhan

Rahman

Mastan

et al. 2018

Gene

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“…Recently, a strain of Indian cassava mosaic virus has been reported to be associated with a mosaic disease of J. curcas in Dharwad, Southern India (Gao et al. 2010), and two strains of African cassava mosaic virus in Kenya (Ramkat et al. 2011a,b).…”

Section: Introductionmentioning

confidence: 99%

Biological Characterization and Complete Genome Sequence of a Possible Strain of Indian cassava mosaic virus from Jatropha curcas in India

Snehi¹,

Srivastava²,

Raj³

2012

Journal of Phytopathology

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The outbreak of a severe mosaic disease with a significant incidence was noticed on Jatropha curcas plants growing in Lucknow, Northern India. The causal virus was successfully transmitted by whiteflies (Bemisia tabaci) and grafting from naturally infected to healthy J. curcas plants. The association of Begomovirus with the mosaic disease of J. curcas was detected by PCR using primers specific to DNA‐A of Begomoviruses. Further, full‐length DNA‐A genome of ∼2.7 kb was amplified by RCA followed by digestion with Bam HI restriction enzyme. Cloning and sequencing of obtained amplicons resulted in 2740 nucleotides of complete DNA‐A consisting of six ORFs and IR region (GenBank Accession ). The sequence analysis revealed highest 85% similarities with Jatropha curcas mosaic virus, 77–84% with Indian cassava mosaic virus and 73–76% with Sri Lankan cassava mosaic virus isolates. Phylogenetic analysis of the Begomovirus isolate also showed a clear‐cut distinct relationship with earlier reported Begomoviruses from Jatropha curcas and other Begomoviruses. On the basis of the guidelines of the International Committee on Taxonomy of Viruses (ICTV‐2008), our virus isolate was identified as a possible strain of Indian cassava mosaic virus, and its name Jatropha mosaic India virus (JMIV) is proposed.

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“…However, recent studies have shown that ACMV can spread from infected cassava to J. curcas plants in the field (Appiah et al, 2012). Additionally, Ramkat et al (2011) have reported the co-infection of J. curcas by ACMV and East African cassava mosaic virus-Ugandan variant (EACMV-UG) in Kenya. These recent reports of the infection of J. curcas by ACMV and EACMV-UG render the use of J. curcas as a hedge crop to trap B. tabaci inappropriate, since virus transmission from J. curcas to cassava may occur.…”

Section: Introductionmentioning

confidence: 99%

Controlled transmission of African cassava mosaic virus (ACMV) by Bemisia tabaci from cassava (Manihot esculenta Crantz) to seedlings of physic nut (Jatropha curcas L.)

Amoatey¹,

Appiah²,

Danso³

et al. 2013

Afr. J. Biotechnol.

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Jatropha curcas, a plant with great biodiesel potential is also used to reduce the population of whiteflies, Bemisia tabaci on cassava fields when planted as a hedge. We therefore, investigated the transmission of African cassava mosaic virus (ACMV) by the whitefly vector from cassava to seedlings of 10 accessions of J. curcas as part of a wider investigation on the possible role of J. curcas as an alternative host of ACMV. Transmission tests were conducted in insect-proof cages using adult B. tabaci collected from ACMV-infected cassava in the field, at a rate of three adult whiteflies per J. curcas seedling and a transmission feeding period of four days. Twenty one (21) days after the infestation, leaf samples from individual plants of the 10 J. curcas accessions were tested for the presence of ACMV by the polymerase chain reaction (PCR) and the double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using the monoclonal antibodies SCRI 33. DAS-ELISA detected ACMV in five out of the ten J. curcas accessions while PCR detected it in eight of the 10 accessions. Furthermore, 18 out of the 35 Nicotiana benthamiana indicator plants mechanically inoculated with sap from symptomatic J. curcas seedlings produced symptoms typical of ACMV infection. This indicates that J. curcas is a likely host of ACMV and it may in turn, be able to infect cassava, and presumably other ACMV-susceptible hosts, in the presence of the vector.

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Biotechnological approaches to determine the impact of viruses in the energy crop plant Jatropha curcas

Cited by 23 publications

References 47 publications

Isolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L.

Isolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L.

Biological Characterization and Complete Genome Sequence of a Possible Strain of Indian cassava mosaic virus from Jatropha curcas in India

Controlled transmission of African cassava mosaic virus (ACMV) by Bemisia tabaci from cassava (Manihot esculenta Crantz) to seedlings of physic nut (Jatropha curcas L.)

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