Biotechnological Approaches in Sugar Beet Development

Philanim, WS; Kumar, Amit; Shettigar, Nivedita

doi:10.1007/978-981-19-2730-0_5

Cited by 3 publications

(1 citation statement)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Abiotic stress is considered one of the major causes of yield loss in most crops worldwide. The loss can reach 50% and more (Philanim et al, 2022). Therefore, in recent years, the development of crop production is aimed at creating agroecosystems capable of rapid response to stressful factors and subsequent self-regulation (Zhuchenko, 2008).…”

Section: Introductionmentioning

confidence: 99%

Obtaining Temperature-Resistant Sugar Beet Lines (<i>Beta vulgaris</i> L.)

Klyachenko¹,

Prysiazhniuk²,

Bokiy³

et al. 2023

Ecol. Eng. Environ. Technol.

View full text Add to dashboard Cite

This study aimed to investigate the sugar beet genotypes for resistance to hyperthermia and obtain temperatureresistant lines. Nine hybrids and one variety of sugar beet were screened. Cotyledonary leaves and hypocotyls isolated from aseptic seedlings were used for induction of callus and subsequent subcultivation. To create hyperthermic conditions, the callus lines were maintained in thermostats at temperatures of +27 °C, +41 °C, +45 °C, and +47 °C. The effect of high temperatures on the callus tissue was assessed by the specific callus diameter index. The free proline was detected with chromatography. As result of callus tissue exposed to temperatures of +41 °C, 45 °C and 47 °C, on the 9th day of cultivation at high temperatures, significant differences were observed in the size and colouration of the callus tissues. At a moderate temperature (+41 °C), the growth of the callus mass was somewhat higher compared to the control. At a high temperature (+45 °C), the intensity of the growth processes decreased and ceased at a temperature of +47 °C. After transfer and subsequent cultivation of callus tissues in regeneration medium MSR -Murashige and Skoog medium for regeneration, all genotypes demonstrated the formation of morphological structures that initiated the formation of regenerated plants. The number of regenerated plants largely fluctuated over temperatures and almost was not related to genotypes. Consequently, the temperature-resistant lines obtained as a result of extreme heat treatment, differ in terms of the specific diameter of callus.

show abstract

Section: Introductionmentioning

confidence: 99%

Obtaining Temperature-Resistant Sugar Beet Lines (<i>Beta vulgaris</i> L.)

Klyachenko¹,

Prysiazhniuk²,

Bokiy³

et al. 2023

Ecol. Eng. Environ. Technol.

View full text Add to dashboard Cite

show abstract

Conventional and Biotechnological Approaches for the Improvement of Industrial Crops

Altaf,

Liaqat,

Ali

et al. 2024

Interdisciplinary Biotechnological Advances

View full text Add to dashboard Cite

Genetic diversity and genome-wide association study of 13 agronomic traits in 977 Beta vulgaris L. germplasms

et al. 2023

View full text Add to dashboard Cite

Background Sugar beet (Beta vulgaris L.) is an economically essential sugar crop worldwide. Its agronomic traits are highly diverse and phenotypically plastic, influencing taproot yield and quality. The National Beet Medium-term Gene Bank in China maintains more than 1700 beet germplasms with diverse countries of origin. However, it lacks detailed genetic background associated with morphological variability and diversity. Results Here, a comprehensive genome-wide association study (GWAS) of 13 agronomic traits was conducted in a panel of 977 sugar beet accessions. Almost all phenotypic traits exhibited wide genetic diversity and high coefficient of variation (CV). A total of 170,750 high-quality single-nucleotide polymorphisms (SNPs) were obtained using the genotyping-by-sequencing (GBS). Neighbour-joining phylogenetic analysis, principal component analysis, population structure and kinship showed no obvious relationships among these genotypes based on subgroups or regional sources. GWAS was carried out using a mixed linear model, and 159 significant associations were detected for these traits. Within the 25 kb linkage disequilibrium decay of the associated markers, NRT1/PTR FAMILY 6.3 (BVRB_5g097760); nudix hydrolase 15 (BVRB_8g182070) and TRANSPORT INHIBITOR RESPONSE 1 (BVRB_8g181550); transcription factor MYB77 (BVRB_2g023500); and ethylene-responsive transcription factor ERF014 (BVRB_1g000090) were predicted to be strongly associated with the taproot traits of root groove depth (RGD); root shape (RS); crown size (CS); and flesh colour (FC), respectively. For the aboveground traits, UDP-glycosyltransferase 79B6 (BVRB_9g223780) and NAC domain-containing protein 7 (BVRB_5g097990); F-box protein At1g10780 (BVRB_6g140760); phosphate transporter PHO1 (BVRB_3g048660); F-box protein CPR1 (BVRB_8g181140); and transcription factor MYB77 (BVRB_2g023500) and alcohol acyltransferase 9 (BVRB_2g023460) might be associated with the hypocotyl colour (HC); plant type (PT); petiole length (PL); cotyledon size (C); and fascicled leaf type (FLT) of sugar beet, respectively. AP-2 complex subunit mu (BVRB_5g106130), trihelix transcription factor ASIL2 (BVRB_2g041790) and late embryogenesis abundant protein 18 (BVRB_5g106150) might be involved in pollen quantity (PQ) variation. The candidate genes extensively participated in hormone response, nitrogen and phosphorus transportation, secondary metabolism, fertilization and embryo maturation. Conclusions The genetic basis of agronomical traits is complicated in heterozygous diploid sugar beet. The putative valuable genes found in this study will help further elucidate the molecular mechanism of each phenotypic trait for beet breeding.

show abstract

Biotechnological Approaches in Sugar Beet Development

Cited by 3 publications

References 86 publications

Obtaining Temperature-Resistant Sugar Beet Lines (<i>Beta vulgaris</i> L.)

Obtaining Temperature-Resistant Sugar Beet Lines (<i>Beta vulgaris</i> L.)

Conventional and Biotechnological Approaches for the Improvement of Industrial Crops

Genetic diversity and genome-wide association study of 13 agronomic traits in 977 Beta vulgaris L. germplasms

Contact Info

Product

Resources

About